Prolonged cytokine exposure causes a dynamic redistribution of endothelial cell adhesion molecules to intercellular junctions.
Bradley JR, Pober JS. Prolonged cytokine exposure causes a dynamic redistribution of endothelial cell adhesion molecules to intercellular junctions. Laboratory Investigation 1996, 75: 463-72. PMID: 8874379.Peer-Reviewed Original ResearchConceptsCell junctionsProtein redistributionMembrane protein redistributionLongitudinal stress fibersDynamic redistributionIntercellular adhesion molecule-2Cultured umbilical vein endothelial cellsBeta 3 integrinAdhesion moleculesBeta 1 integrinCell adhesion moleculeDense peripheral bandStress fibersCytoskeletal actinEndothelial cellsUmbilical vein endothelial cellsActin filamentsCell adhesion molecule-1Vein endothelial cellsAdhesion molecule-1Intercellular adhesion molecule 1 moleculesIntercellular junctionsApical surfaceVascular cell adhesion molecule-1Peripheral band"Cytosolic" phospholipase A2 is in the nucleus of subconfluent endothelial cells but confined to the cytoplasm of confluent endothelial cells and redistributes to the nuclear envelope and cell junctions upon histamine stimulation.
Sierra-Honigmann MR, Bradley JR, Pober JS. "Cytosolic" phospholipase A2 is in the nucleus of subconfluent endothelial cells but confined to the cytoplasm of confluent endothelial cells and redistributes to the nuclear envelope and cell junctions upon histamine stimulation. Laboratory Investigation 1996, 74: 684-95. PMID: 8600319.Peer-Reviewed Original ResearchConceptsSubconfluent endothelial cellsConfluent endothelial cellsNuclear envelopeNuclear localizationConfocal immunofluorescence microscopyEndothelial cellsDose-dependent redistributionGrowth-arrested cellsSubcellular localizationCell density dependencePlasma membraneCell cycleCytoplasmic enzymeNuclear extractsCell junctionsPhospholipase A2 enzymeBovine endothelial cellsHeLa cellsImmunofluorescence microscopyCell lysatesCell nucleiIntercellular junctionsSubconfluent cellsPredominant MrAcid metabolism