2021
MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells
Jeong J, Shin JH, Li W, Hong JY, Lim J, Hwang JY, Chung JJ, Yan Q, Liu Y, Choi J, Wysolmerski J. MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells. Cell Reports 2021, 37: 110160. PMID: 34965434, PMCID: PMC8762588, DOI: 10.1016/j.celrep.2021.110160.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Agents, ImmunologicalBreast NeoplasmsCell ProliferationCytoskeletal ProteinsDrug Resistance, NeoplasmEndocytosisFemaleHumansMembrane MicrodomainsMyelin and Lymphocyte-Associated Proteolipid ProteinsPhosphoproteinsPlasma Membrane Calcium-Transporting ATPasesReceptor, ErbB-2Sodium-Hydrogen ExchangersTrastuzumabTumor Cells, CulturedConceptsLipid raft formationBreast cancer cellsLipid raftsLipid raft resident proteinsCancer cellsRaft formationRaft-resident proteinsProximity ligation assayProtein complexesMembrane protrusionsProtein interactionsPlasma membraneLigation assayMAL2Membrane stabilityStructural organizationPotential therapeutic targetPhysical interactionMembrane retentionProteinRaftsTherapeutic targetCellsIntracellular calcium concentrationLow intracellular calcium concentration
2002
Overexpression of Parathyroid Hormone‐Related Protein Causes Hypercalcemia but Not Bone Metastases in a Murine Model of Mammary Tumorigenesis*
Wysolmerski JJ, Dann PR, Zelazny E, Dunbar ME, Insogna KL, Guise TA, Perkins AS. Overexpression of Parathyroid Hormone‐Related Protein Causes Hypercalcemia but Not Bone Metastases in a Murine Model of Mammary Tumorigenesis*. Journal Of Bone And Mineral Research 2002, 17: 1164-1170. PMID: 12096830, DOI: 10.1359/jbmr.2002.17.7.1164.Peer-Reviewed Original ResearchConceptsBone metastasesBreast cancer cellsCancer cellsTransgenic miceTumor-bearing transgenic miceParathyroid hormone-related proteinPrimary breast cancerProduction of PTHrPHormone-related proteinWild-type littermatesEffects of PTHrPCause hypercalcemiaWild-type controlsVisceral metastasesSkeletal metastasesParathyroid hormoneBone resorptionArterial circulationBreast cancerDMBA treatmentMammary carcinogenImmunodeficient miceMammary tumorigenesisMurine modelHigh incidence
2000
Activation of PTHrP Gene Expression in Squamous Carcinoma Cell Lines by Mutant Isoforms of the Tumor Suppressor p53
Foley J, King CS, Jiménez JA, Wysolmerski JJ, Philbrick WM. Activation of PTHrP Gene Expression in Squamous Carcinoma Cell Lines by Mutant Isoforms of the Tumor Suppressor p53. Oncology Research Featuring Preclinical And Clinical Cancer Therapeutics 2000, 12: 71-81. PMID: 11132926, DOI: 10.3727/096504001108747549.Peer-Reviewed Original ResearchAllelesAnimalsBlotting, WesternCarcinoma, Squamous CellCodonGene Expression Regulation, NeoplasticGenes, p53HumansMiceMutagenesis, Site-DirectedMutationMutation, MissenseParathyroid Hormone-Related ProteinPlasmidsPoint MutationPrecipitin TestsPromoter Regions, GeneticProtein ConformationProtein DenaturationProtein IsoformsProteinsRNA, MessengerTransfectionTumor Cells, CulturedTumor Suppressor Protein p53
1996
Parathyroid hormone-related protein gene expression in human squamous carcinoma cells is repressed by mutant isoforms of p53.
Foley J, Wysolmerski JJ, Broadus AE, Philbrick WM. Parathyroid hormone-related protein gene expression in human squamous carcinoma cells is repressed by mutant isoforms of p53. Cancer Research 1996, 56: 4056-62. PMID: 8752179.Peer-Reviewed Original ResearchMeSH KeywordsAdenovirus E1B ProteinsCarcinoma, Squamous CellGene ExpressionGenes, p53Genes, ReporterHumansIsomerismKeratinocytesMutationParathyroid Hormone-Related ProteinPromoter Regions, GeneticProtein BiosynthesisProteinsRNA, MessengerTATA BoxTransfectionTumor Cells, CulturedTumor Suppressor Protein p53ConceptsHormone-related protein gene expressionPTHrP expression levelsSquamous carcinoma linesHormone-related proteinNormal secretory productP53 functional statusSquamous carcinoma cellsPTHrP gene expressionHuman squamous carcinoma cellsMutant p53 isoformsHumoral hypercalcemiaSquamous carcinomaFunctional statusPTHrP expressionPTHrP mRNASquamous epitheliumEndogenous mutant p53Mutant isoformsP53 isoformsCarcinoma linesWild-type p53Carcinoma cellsGene expressionCodon 248PTHrP transcriptsTransactivation of the PTHrP gene in squamous carcinomas predicts the occurrence of hypercalcemia in athymic mice.
Wysolmerski JJ, Vasavada R, Foley J, Weir EC, Burtis WJ, Kukreja SC, Guise TA, Broadus AE, Philbrick WM. Transactivation of the PTHrP gene in squamous carcinomas predicts the occurrence of hypercalcemia in athymic mice. Cancer Research 1996, 56: 1043-9. PMID: 8640759.Peer-Reviewed Original ResearchConceptsPTHrP gene expressionPTHrP mRNA expressionSquamous tumorsTumor linesAthymic miceMRNA expressionParathyroid hormone-related proteinOccurrence of hypercalcemiaPTHrP mRNA levelsHormone-related proteinSquamous histologyGene expressionPTHrP secretionHumoral hypercalcemiaSquamous carcinomaPTHrP gene promoterPTHrP mRNAHypercalcemiaSecretion ratePTHrP geneTumor cellsTumorsMRNA levelsHigh levelsHHM