2018
Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation
Zwick RK, Rudolph MC, Shook BA, Holtrup B, Roth E, Lei V, Van Keymeulen A, Seewaldt V, Kwei S, Wysolmerski J, Rodeheffer MS, Horsley V. Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation. Nature Communications 2018, 9: 3592. PMID: 30181538, PMCID: PMC6123393, DOI: 10.1038/s41467-018-05911-0.Peer-Reviewed Original ResearchConceptsMouse mammary glandMilk-producing epithelial cellsTissue-specific rolesMammary glandAdipose growthLipid dynamicsGenetic tracingPhysiological examplesFunctional implicationsCellular mechanismsAdipocyte hypertrophyMature adipocytesEssential roleVivo analysisTissue functionMammary epitheliumAdipocytesEpithelial cellsAdipocyte precursorsSubsequent involutionMilk lipidsPrimary mechanismMechanismLipidomicsMilk fat production
2011
Parathyroid Hormone-Related Protein Is Not Required for Normal Ductal or Alveolar Development in the Post-Natal Mammary Gland
Boras-Granic K, VanHouten J, Hiremath M, Wysolmerski J. Parathyroid Hormone-Related Protein Is Not Required for Normal Ductal or Alveolar Development in the Post-Natal Mammary Gland. PLOS ONE 2011, 6: e27278. PMID: 22087279, PMCID: PMC3210770, DOI: 10.1371/journal.pone.0027278.Peer-Reviewed Original ResearchConceptsMammary glandPTHrP expressionAlveolar developmentMyoepithelial cellsMaternal calcium homeostasisOverexpression of PTHrPRole of PTHrPExpression of PTHrPEmbryonic mammary budPTHrP deficiencyMouse mammary glandParathyroid hormoneTransgenic miceDuctal developmentCalcium homeostasisAlveolar cellsPTHrPPubertal developmentMMTV-CrePost-natal mammary glandMammary developmentMammary budPostnatal mammary glandCre transgenePTHrP geneSkeletal recovery after weaning does not require PTHrP*
Kirby BJ, Ardeshirpour L, Woodrow JP, Wysolmerski JJ, Sims NA, Karaplis AC, Kovacs CS. Skeletal recovery after weaning does not require PTHrP*. Journal Of Bone And Mineral Research 2011, 26: 1242-1251. PMID: 21308774, PMCID: PMC3179289, DOI: 10.1002/jbmr.339.Peer-Reviewed Original ResearchConceptsBone mineral contentParathyroid hormoneBone massSerum calciumLumbar spine bone mineral contentTrabecular bone mineral contentParathyroid hormone-related proteinSpine bone mineral contentBone formationNormal serum calciumLow bone massAdult bone massPTHrP mRNA expressionWild-type miceHormone-related proteinEnd of lactationType 1 collagenUrine calciumSkeletal recoveryPTHrP mRNANull miceMRNA expressionFull recoveryMiceConditional knockout
2010
Characterization of the Six1 homeobox gene in normal mammary gland morphogenesis
Coletta RD, McCoy EL, Burns V, Kawakami K, McManaman JL, Wysolmerski JJ, Ford HL. Characterization of the Six1 homeobox gene in normal mammary gland morphogenesis. BMC Developmental Biology 2010, 10: 4. PMID: 20074369, PMCID: PMC2823684, DOI: 10.1186/1471-213x-10-4.Peer-Reviewed Original ResearchConceptsNormal mammary gland developmentAdult mammary glandMammary gland developmentMammary glandMammary developmentBreast cancerMouse modelBreast tumorigenesisEmbryonic mammary glandPostnatal mammary developmentRag1-/- miceNormal mammary gland morphogenesisGland developmentTransgenic mouse modelBreast cancer initiationViable therapeutic targetIndependent mouse modelsAdult mouse mammary glandAnti-cancer therapyMammary stem cellsFamily membersMouse mammary glandUnwanted side effectsRole of Six1Invasive carcinoma
2008
Switching of G-protein Usage by the Calcium-sensing Receptor Reverses Its Effect on Parathyroid Hormone-related Protein Secretion in Normal Versus Malignant Breast Cells*
Mamillapalli R, VanHouten J, Zawalich W, Wysolmerski J. Switching of G-protein Usage by the Calcium-sensing Receptor Reverses Its Effect on Parathyroid Hormone-related Protein Secretion in Normal Versus Malignant Breast Cells*. Journal Of Biological Chemistry 2008, 283: 24435-24447. PMID: 18621740, PMCID: PMC2528989, DOI: 10.1074/jbc.m801738200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone NeoplasmsBreast NeoplasmsCell Line, TumorCyclic AMPCyclic AMP-Dependent Protein KinasesFemaleGTP-Binding Protein alpha SubunitsHumansHypercalcemiaLactationMammary Glands, AnimalMammary Neoplasms, AnimalMAP Kinase Signaling SystemMiceNeoplasm MetastasisNeoplasm ProteinsParathyroid Hormone-Related ProteinPregnancyReceptors, Calcium-SensingType C PhospholipasesConceptsG-protein usagePTHrP productionMammary epithelial cellsBreast cancer cellsBreast cellsMCF-7 cellsParathyroid hormone-related protein secretionParathyroid hormone-related proteinParathyroid hormone secretionCancer cellsMCF-7 human breast cancer cellsCalcium-sensing receptorHuman breast cancer cellsHormone-related proteinNormal mammary epithelial cellsRegulation of calciumMalignant breast cellsNormal breast cellsG protein-coupled receptorsG protein couplingBone metastasesPTHrP secretionHormone secretionBone metabolismBreast cancer
2007
Weaning Triggers a Decrease in Receptor Activator of Nuclear Factor-κB Ligand Expression, Widespread Osteoclast Apoptosis, and Rapid Recovery of Bone Mass after Lactation in Mice
Ardeshirpour L, Dann P, Adams DJ, Nelson T, VanHouten J, Horowitz MC, Wysolmerski JJ. Weaning Triggers a Decrease in Receptor Activator of Nuclear Factor-κB Ligand Expression, Widespread Osteoclast Apoptosis, and Rapid Recovery of Bone Mass after Lactation in Mice. Endocrinology 2007, 148: 3875-3886. PMID: 17495007, DOI: 10.1210/en.2006-1467.Peer-Reviewed Original ResearchConceptsBone lossBone massBone turnoverReceptor activatorOsteoclast apoptosisRapid bone lossC-telopeptide levelsNumber of osteoclastsBone formation rateNuclear factor-kappaB ligand (RANKL) mRNANuclear factor-kappaB ligand (RANKL) expressionRapid recoveryLevels of osteocalcinCessation of lactationSkeletal anabolismPTH levelsCalcium metabolismBone resorptionBone histomorphometryBone microarchitectureCalcium levelsOsteoblast numberLigand expressionReciprocal decreaseBone recovery
2005
Hypercalcemia in Breast Cancer: An Echo of Bone Mobilization During Lactation?
DeMauro S, Wysolmerski J. Hypercalcemia in Breast Cancer: An Echo of Bone Mobilization During Lactation? Journal Of Mammary Gland Biology And Neoplasia 2005, 10: 157-167. PMID: 16025222, DOI: 10.1007/s10911-005-5398-9.BooksConceptsBreast cancer patientsBone resorptionCancer patientsBreast cancerParathyroid hormone-related proteinPathophysiology of hypercalcemiaMultiple skeletal metastasesPathological bone resorptionHormone-related proteinActivation of osteoclastsSecretion of cytokinesClass of drugsSkeletal calcium storesNormal breast cellsFrequent complicationSkeletal metastasesSignificant morbidityCalcium metabolismCurrent therapiesHypercalcemiaNew therapiesCalcium levelsOsteoclast differentiationCalcium storesPatients
2004
The calcium-sensing receptor regulates mammary gland parathyroid hormone–related protein production and calcium transport
VanHouten J, Dann P, McGeoch G, Brown EM, Krapcho K, Neville M, Wysolmerski JJ. The calcium-sensing receptor regulates mammary gland parathyroid hormone–related protein production and calcium transport. Journal Of Clinical Investigation 2004, 113: 598-608. PMID: 14966569, PMCID: PMC338258, DOI: 10.1172/jci18776.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related protein productionCalcium-sensing receptorMammary epithelial cellsEpithelial cellsCalcium transportMilk calcium contentExtracellular calcium concentrationSecretion of calciumTranscellular calcium transportPTHrP secretionPTHrP productionExtracellular calciumTransfer of calciumCalcium contentMammary glandCalcium concentrationProtein concentrationAvailability of calciumHomeostatic systemConcentration of calciumMilk osmolalitySecretionCalciumReceptorsThree-dimensional culture system
2002
The Evolutionary Origins of Maternal Calcium and Bone Metabolism During Lactation
Wysolmerski JJ. The Evolutionary Origins of Maternal Calcium and Bone Metabolism During Lactation. Journal Of Mammary Gland Biology And Neoplasia 2002, 7: 267-276. PMID: 12751891, DOI: 10.1023/a:1022800716196.BooksConceptsLower vertebratesEvolutionary originEutherian mammalsBone metabolismBone resorptionMaternal adaptationDifferent speciesMammalsMaternal calcium homeostasisRenal calcium excretionVertebratesAbsorption of calciumCalcium homeostasisEgg productionMetabolismMaternal calciumCalcium excretionHuman pregnancyMaternal transferCalcium metabolismFetal growthMineral metabolismNursing mothersAdaptationPregnancy
1999
Parathyroid Hormone-Related Protein: A Developmental Regulatory Molecule Necessary for Mammary Gland Development
Dunbar M, Wysolmerski J. Parathyroid Hormone-Related Protein: A Developmental Regulatory Molecule Necessary for Mammary Gland Development. Journal Of Mammary Gland Biology And Neoplasia 1999, 4: 21-34. PMID: 10219904, DOI: 10.1023/a:1018700502518.Peer-Reviewed Original Research
1998
THE PHYSIOLOGY OF PARATHYROID HORMONE-RELATED PROTEIN: An Emerging Role as a Developmental Factor
Wysolmerski JJ, Stewart AF. THE PHYSIOLOGY OF PARATHYROID HORMONE-RELATED PROTEIN: An Emerging Role as a Developmental Factor. Annual Review Of Physiology 1998, 60: 431-460. PMID: 9558472, DOI: 10.1146/annurev.physiol.60.1.431.BooksConceptsRole of PTHrPHormone-related proteinVascular smooth muscle toneParathyroid hormone-related proteinSmooth muscle tonePancreatic islet massMechanism of actionIntracrine regulatorHumoral hypercalcemiaMuscle tonePathogenic roleIslet massPTHrPDevelopmental factorsMammary glandEmerging RoleCalcium transferCell deathNormal developmentalAdult physiologySecretory formCell growthHypercalcemiaMalignancySyndrome