2021
Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism
Rao Q, Han L, Wang Y, Chai P, Kuo YW, Yang R, Hu F, Yang Y, Howard J, Zhang K. Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism. Nature Structural & Molecular Biology 2021, 28: 799-810. PMID: 34556869, PMCID: PMC8500839, DOI: 10.1038/s41594-021-00656-9.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCryoelectron MicroscopyDyneinsMicrotubulesModels, MolecularTetrahymena thermophilaConceptsOuter arm dyneinMicrotubule doubletsDistinct microtubule-binding domainsHigh-resolution structuresAction of dyneinsMicrotubule-binding domainNative tracksATP hydrolysisDynein motorsHydrolyze ATPConformational changesNucleotide cycleMotor coordination mechanismATP turnoverDyneinHead interactionsMechanical forces
2019
Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules
Kuo YW, Trottier O, Mahamdeh M, Howard J. Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 5533-5541. PMID: 30837315, PMCID: PMC6431158, DOI: 10.1073/pnas.1818824116.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAdenosine TriphosphateAnimalsDrosophila melanogasterDrosophila ProteinsMicrotubulesSpastinConceptsDual-function enzymeMicrotubule dynamicsMass of microtubulesMicrotubule cytoskeletonDynamic cellular processesCellular processesMicrotubule regrowthNeuronal morphogenesisSevering activityVivo phenotypeMicrotubule assaysMicrotubulesNumber of microtubulesSpastinMicrotubule fragmentsRescue frequencyShort fragmentsCytoskeletonMicrotubule massObserved exponential increaseEnzymeRemodelersFidgetinKataninCiliogenesis
2006
The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends
Helenius J, Brouhard G, Kalaidzidis Y, Diez S, Howard J. The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends. Nature 2006, 441: 115-119. PMID: 16672973, DOI: 10.1038/nature04736.Peer-Reviewed Original Research
1994
The force exerted by a single kinesin molecule against a viscous load
Hunt AJ, Gittes F, Howard J. The force exerted by a single kinesin molecule against a viscous load. Biophysical Journal 1994, 67: 766-781. PMID: 7948690, PMCID: PMC1225420, DOI: 10.1016/s0006-3495(94)80537-5.Peer-Reviewed Original Research