2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegeneration
2018
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane
Cai H, Gong J, Del Priore LV, Tezel TH, Fields MA. Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane. Journal Of Visualized Experiments 2018, 57084. PMID: 29708536, PMCID: PMC5933494, DOI: 10.3791/57084.Peer-Reviewed Original ResearchConceptsAtrophic age-related macular degenerationInduced pluripotent stem cellsRetinal pigment epithelial cellsEx vivo modelBruch's membranePigment epithelial cellsVivo modelGeographic atrophyHuman Bruch's membraneEstablishment of polarityHuman Bruch's membrane explantsRPE cellsAge-related macular degenerationMembrane pathologySufficient animal modelsEpithelial cellsEye Disease StudyPluripotent stem cellsAged Bruch's membraneBruch's membrane explantsPrimary cell linesAged human Bruch's membranePhotoreceptor outer segmentsGene expressionEffective therapy
2016
Potential of Induced Pluripotent Stem Cells (iPSCs) for Treating Age-Related Macular Degeneration (AMD)
Fields M, Cai H, Gong J, Del Priore L. Potential of Induced Pluripotent Stem Cells (iPSCs) for Treating Age-Related Macular Degeneration (AMD). Cells 2016, 5: 44. PMID: 27941641, PMCID: PMC5187528, DOI: 10.3390/cells5040044.Peer-Reviewed Original ResearchInduced pluripotent stem cellsEmbryonic stem cellsAge-related macular degenerationPluripotent stem cellsCell replacement therapyDisease modelingIPSC disease modelingStem cellsReplacement therapyMacular degenerationRegenerative medicineRetinal pigment epithelium cellsStem cell biologyPigment epithelium cellsRetinal degenerative disordersUnlimited sourceDrug development platformClinical trialsDevelopment platformStargardt diseaseAnimal modelsRetinal degenerationAutologous sourceDegenerative disordersTherapeutic use