2010
Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2
Cardin JA, Carlén M, Meletis K, Knoblich U, Zhang F, Deisseroth K, Tsai LH, Moore CI. Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2. Nature Protocols 2010, 5: 247-254. PMID: 20134425, PMCID: PMC3655719, DOI: 10.1038/nprot.2009.228.Peer-Reviewed Original ResearchConceptsOptical interferenceMajor long-term goalViral vectorsCell-type selectivityRecording of neuronsOptogenetic stimulationChannelrhodopsin-2Cre-dependent expressionBrain circuit functionSelective cell typesInhibitory interneuronsIntracellular recordingsVivo electrophysiologyExcitatory neuronsIntact brainType selectivityNeural subtypesOptogenetic techniquesSpecific populationsNeural activityCircuit functionNeuronsInterferenceCell typesStimulation
2008
Cellular Mechanisms Underlying Stimulus-Dependent Gain Modulation in Primary Visual Cortex Neurons In Vivo
Cardin JA, Palmer LA, Contreras D. Cellular Mechanisms Underlying Stimulus-Dependent Gain Modulation in Primary Visual Cortex Neurons In Vivo. Neuron 2008, 59: 150-160. PMID: 18614036, PMCID: PMC2504695, DOI: 10.1016/j.neuron.2008.05.002.Peer-Reviewed Original ResearchConceptsSynaptic activationPrimary visual cortex neuronsCat primary visual cortexVisual cortex neuronsCellular mechanismsPrimary visual cortexNeuronal gainBackground synaptic activityVisual stimuliPostsynaptic effectsCortex neuronsIntracellular recordingsSynaptic activitySynaptic inputsVisual cortexInput resistanceVisual stimulationGain modulationSustained changesVivoSensory contextMembrane potentialNeuronal phenomenaActivationModulation