2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2015
Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment
Gruber DF, Gaffney JP, Mehr S, DeSalle R, Sparks JS, Platisa J, Pieribone VA. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment. PLOS ONE 2015, 10: e0140972. PMID: 26561348, PMCID: PMC4641735, DOI: 10.1371/journal.pone.0140972.Peer-Reviewed Original ResearchConceptsAcid-binding proteinFluorescent proteinFatty acid-binding proteinDuplication eventsGene duplication eventsStrong positive selectionNew fluorescent proteinsMarine environmentAdaptive evolutionEvolutionary switchCryptic genusVertebrate ordersVertebrate taxaPhylogenetic reconstructionProtein familyPhylogenetic analysisSequence motifsSister groupEvolutionary adaptationTranscriptomic analysisPositive selectionFatty acid binding proteinAcid binding proteinBinding proteinBright green fluorescence