2009
Peripheral glial cell differentiation from neurospheres derived from adipose mesenchymal stem cells
Radtke C, Schmitz B, Spies M, Kocsis J, Vogt P. Peripheral glial cell differentiation from neurospheres derived from adipose mesenchymal stem cells. International Journal Of Developmental Neuroscience 2009, 27: 817-823. PMID: 19699793, DOI: 10.1016/j.ijdevneu.2009.08.006.Peer-Reviewed Original ResearchConceptsMesenchymal stem cellsStem cellsGlial-like cellsAdipose-derived mesenchymal stem cellsGlial cell differentiationPeripheral glial cellsGrowth factorEpidermal growth factorGrowth factor receptorMitogen withdrawalFibroblast growth factorBasic fibroblast growth factorCell differentiationDorsal root ganglion neuronsSchwann cell marker S100P75 nerve growth factor receptorAdipose-derived stem cellsNerve growth factor receptorCellular aggregatesSimultaneous expressionOlfactory Ensheathing CellsAppropriate inductionFactor receptorNeurospheresGlia markers
2000
Nerve growth factor maintains potassium conductance after nerve injury in adult cutaneous afferent dorsal root ganglion neurons
Everill B, Kocsis J. Nerve growth factor maintains potassium conductance after nerve injury in adult cutaneous afferent dorsal root ganglion neurons. Neuroscience 2000, 100: 417-422. PMID: 11008179, PMCID: PMC2605351, DOI: 10.1016/s0306-4522(00)00263-3.Peer-Reviewed Original ResearchConceptsTransient A-currentNerve growth factorDorsal root ganglion neuronsK currentsNerve ligationGrowth factorA-currentGanglion neuronsSciatic nerveWhole-cell patch-clamp techniquePotassium conductanceDistal nerve segmentsAfferent cell bodiesMini-osmotic pumpsVoltage-dependent potassium conductancePatch-clamp techniqueTransient potassium currentAppropriate ion replacementNerve growth factor treatmentGrowth factor treatmentNerve injuryNerve crushNerve segmentsCutaneous afferentsControl neuronsVoltage-Gated Calcium Currents in Axotomized Adult Rat Cutaneous Afferent Neurons
Baccei M, Kocsis J. Voltage-Gated Calcium Currents in Axotomized Adult Rat Cutaneous Afferent Neurons. Journal Of Neurophysiology 2000, 83: 2227-2238. PMID: 10758131, DOI: 10.1152/jn.2000.83.4.2227.Peer-Reviewed Original ResearchConceptsVoltage-gated calcium currentsCutaneous afferent neuronsPA/pFNerve injuryCutaneous afferentsType 1 cellsBarium currentsAfferent neuronsHVA currentsCalcium currentLow threshold T-type currentWhole-cell patch-clamp techniqueDorsal root ganglion neuronsCell patch-clamp techniqueFluoro-Gold labelingOmega-agatoxin TKSciatic nerve injuryOmega-conotoxin GVIAPercentage of neuronsType 2 phenotypeT-type currentType 2 neuronsCalcium current propertiesType 1 neuronsControl cellsExcitability changes of dorsal root axons following nerve injury: implications for injury-induced changes in axonal Na+ channels
Nonaka T, Honmou O, Sakai J, Hashi K, Kocsis J. Excitability changes of dorsal root axons following nerve injury: implications for injury-induced changes in axonal Na+ channels. Brain Research 2000, 859: 280-285. PMID: 10719075, DOI: 10.1016/s0006-8993(00)01979-x.Peer-Reviewed Original ResearchConceptsDorsal root axonsNerve injuryDorsal rootsAction potentialsCutaneous afferent axonsSciatic nerve ligationAfferent cell bodiesInjury-induced changesRat dorsal rootSucrose gap chamberTarget disconnectionNerve ligationExcitability changesSpinal cordAfferent axonsTrophic supportRoot ligationRefractory periodCell bodiesElectrophysiological recordingsAxonsInjuryDepolarizationLigationPrevious studies
1999
Reduction in Potassium Currents in Identified Cutaneous Afferent Dorsal Root Ganglion Neurons After Axotomy
Everill B, Kocsis J. Reduction in Potassium Currents in Identified Cutaneous Afferent Dorsal Root Ganglion Neurons After Axotomy. Journal Of Neurophysiology 1999, 82: 700-708. PMID: 10444667, DOI: 10.1152/jn.1999.82.2.700.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxotomyGanglia, SpinalNeurons, AfferentPatch-Clamp TechniquesPotassium ChannelsRatsRats, WistarSkinConceptsDorsal root ganglion neuronsCutaneous afferent neuronsAfferent neuronsGanglion neuronsPotassium currentWhole-cell patch-clamp recordingsCell patch-clamp recordingsPrimary afferent neuronsEffects of axotomyPatch-clamp recordingsControl cellsAppropriate ion replacementAcute ligationNerve ligationDRG neuronsExcitability changesChannel blockersNeuronal excitabilityPharmacological componentsAxotomyNeuronsLigationBasis of sensitivityCellsBlockers
1998
Morphologically Identified Cutaneous Afferent DRG Neurons Express Three Different Potassium Currents in Varying Proportions
Everill B, Rizzo M, Kocsis J. Morphologically Identified Cutaneous Afferent DRG Neurons Express Three Different Potassium Currents in Varying Proportions. Journal Of Neurophysiology 1998, 79: 1814-1824. PMID: 9535950, PMCID: PMC2605378, DOI: 10.1152/jn.1998.79.4.1814.Peer-Reviewed Original ResearchConceptsDRG neuronsMedium-sized DRG neuronsDorsal root ganglion neuronsCutaneous afferent neuronsDifferent potassium currentsWhole-cell patch-clamp configurationOutward current componentPatch-clamp configurationAfferent neuronsFluoro-GoldGanglion neuronsRetrograde labelingConditioning prepulseEntire populationChannel blockersPotassium currentMyelinated axonsSustained currentNeuronsTest pulseDendrotoxinBasis of sensitivityIKCellsBlockersPeripheral Axotomy Induces Long-Term c-Jun Amino-Terminal Kinase-1 Activation and Activator Protein-1 Binding Activity by c-Jun and junD in Adult Rat Dorsal Root Ganglia In Vivo
Kenney A, Kocsis J. Peripheral Axotomy Induces Long-Term c-Jun Amino-Terminal Kinase-1 Activation and Activator Protein-1 Binding Activity by c-Jun and junD in Adult Rat Dorsal Root Ganglia In Vivo. Journal Of Neuroscience 1998, 18: 1318-1328. PMID: 9454841, PMCID: PMC2605350, DOI: 10.1523/jneurosci.18-04-01318.1998.Peer-Reviewed Original ResearchConceptsC-Jun proteinC-JunC-Jun amino-terminal kinase 1Nerve injuryAxonal regenerationAmino-terminal phosphorylationLumbar dorsal root ganglion neuronsActivator protein-1 bindingEarly post-injury periodJun kinase activationAdult rat dorsal root gangliaDorsal root ganglion neuronsRat dorsal root gangliaKinase 1 activationC-Jun phosphorylationSciatic nerve injuryActivator protein-1 binding activityPost-injury periodSciatic nerve transectionAdult rat DRGDorsal root gangliaLong-term upregulationPeripheral axonal regenerationAP-1 bindingProtein-1 bindingMechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion
Lankford K, Waxman S, Kocsis J. Mechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion. The Journal Of Comparative Neurology 1998, 391: 11-29. PMID: 9527536, PMCID: PMC2605358, DOI: 10.1002/(sici)1096-9861(19980202)391:1<11::aid-cne2>3.0.co;2-u.Peer-Reviewed Original ResearchConceptsDorsal root gangliaConditioning lesionNerve injuryNerve regenerationAffected dorsal root ganglionControl dorsal root gangliaDenervated peripheral nervePrior nerve injurySciatic nerve lesionCultured DRG neuronsSciatic nerve transectionPeripheral target tissuesPeripheral nerve stumpRapid nerve regenerationAbility of neuronsSecond axotomyNerve lesionsDRG neuronsNerve transectionNerve stumpRoot gangliaControl neuronsPeripheral nervesNerve tractsAdult rats
1997
Temporal variability of jun family transcription factor levels in peripherally or centrally transected adult rat dorsal root ganglia
Kenney A, Kocsis J. Temporal variability of jun family transcription factor levels in peripherally or centrally transected adult rat dorsal root ganglia. Brain Research 1997, 52: 53-61. PMID: 9450677, DOI: 10.1016/s0169-328x(97)00211-8.Peer-Reviewed Original ResearchConceptsDorsal root gangliaDorsal root transectionPeripheral axotomyDRG neuronsRoot transectionRoot gangliaProtein levelsAdult rat lumbar dorsal root gangliaRat lumbar dorsal root gangliaLumbar dorsal root gangliaAdult rat dorsal root gangliaRat dorsal root gangliaNeurotrophic factor administrationSpinal nerve transectionC-Jun protein levelsAdministration of NGFFactor protein levelsJunD protein levelsTarget disconnectionNerve transectionFactor administrationPeripheral branchesTransectionFactor levelsRapid elevationDifferential Effects of NGF and BDNF on Axotomy-Induced Changes in GABAA-Receptor-Mediated Conductance and Sodium Currents in Cutaneous Afferent Neurons
Oyelese A, Rizzo M, Waxman S, Kocsis J. Differential Effects of NGF and BDNF on Axotomy-Induced Changes in GABAA-Receptor-Mediated Conductance and Sodium Currents in Cutaneous Afferent Neurons. Journal Of Neurophysiology 1997, 78: 31-42. PMID: 9242258, PMCID: PMC2605357, DOI: 10.1152/jn.1997.78.1.31.Peer-Reviewed Original ResearchConceptsBrain-derived neurotrophic factorCutaneous afferent neuronsNerve growth factorReceptor-mediated conductanceProportion of neuronsAfferent neuronsAction potential waveformSodium currentNeurotrophic factorL4/L5 DRG neuronsAction potentialsVoltage-dependent sodium currentsWhole-cell patch-clamp techniqueDorsal root ganglion neuronsCell patch-clamp techniqueAxotomy-induced increaseFluoro-Gold injectionsL5 DRG neuronsSpecific neurotrophic factorsSciatic nerve stumpsTTX-sensitive currentsInjury-induced changesResistant sodium currentsGamma-aminobutyric acidPatch-clamp techniqueTiming of c-jun protein induction in lumbar dorsal root ganglia after sciatic nerve transection varies with lesion distance
Kenney A, Kocsis J. Timing of c-jun protein induction in lumbar dorsal root ganglia after sciatic nerve transection varies with lesion distance. Brain Research 1997, 751: 90-95. PMID: 9098571, DOI: 10.1016/s0006-8993(96)01402-3.Peer-Reviewed Original ResearchConceptsSciatic nerve transectionNerve transectionDorsal root gangliaNerve injuryRoot gangliaLumbar dorsal root ganglion neuronsL5 dorsal root gangliaLumbar dorsal root gangliaDorsal root ganglion neuronsSciatic nerve injurySciatic nerve crushC-Jun proteinProtein inductionC-Jun responseNerve crushGanglion neuronsImmunohistochemical studyLesion distanceTransectionInduces upregulationEarly increaseC-jun mRNAEarly inductionChemiluminescent immunoblottingGanglia
1996
GABAA-receptor-mediated conductance and action potential waveform in cutaneous and muscle afferent neurons of the adult rat: differential expression and response to nerve injury
Oyelese A, Kocsis J. GABAA-receptor-mediated conductance and action potential waveform in cutaneous and muscle afferent neurons of the adult rat: differential expression and response to nerve injury. Journal Of Neurophysiology 1996, 76: 2383-2392. PMID: 8899611, PMCID: PMC2605353, DOI: 10.1152/jn.1996.76.4.2383.Peer-Reviewed Original ResearchConceptsGABAA receptor-mediated conductanceMuscle afferent neuronsCutaneous afferent neuronsAction potential waveformAfferent neuronsDorsal root gangliaCrush injuryAction potentialsL5 dorsal root gangliaWhole-cell patch-clamp recordingsCell patch-clamp recordingsInjury-induced plasticitySciatic nerve ligationPotential waveformDistal nerve stumpPatch-clamp recordingsGamma-aminobutyric acidPeak GABANerve ligationMuscle afferentsNerve injuryDRG neuronsInjured neuronsNerve stumpRoot gangliaMechanisms of Paresthesiae, Dysesthesiae, and Hyperesthesiae: Role of Na+ Channel Heterogeneity
Rizzo M, Kocsis J, Waxman S. Mechanisms of Paresthesiae, Dysesthesiae, and Hyperesthesiae: Role of Na+ Channel Heterogeneity. European Neurology 1996, 36: 3-12. PMID: 8719643, DOI: 10.1159/000117192.Peer-Reviewed Original ResearchConceptsAxonal injuryCutaneous afferentsDorsal root ganglion neuronsAction potential activityNormal sensory functionEctopic impulsesDRG neuronsClinical syndromeGanglion neuronsSensory functionMembrane excitabilityInjuryNerve impulsesDysesthesiaeChannel physiologyMolecular changesParesthesiaeAfferentsPreliminary evidenceNeuronsEctopicMolecular mechanismsSensory anatomyPotential activityPopulation
1995
Enhancement of GABAA receptor-mediated conductances induced by nerve injury in a subclass of sensory neurons
Oyelese A, Eng D, Richerson G, Kocsis J. Enhancement of GABAA receptor-mediated conductances induced by nerve injury in a subclass of sensory neurons. Journal Of Neurophysiology 1995, 74: 673-683. PMID: 7472373, PMCID: PMC2605359, DOI: 10.1152/jn.1995.74.2.673.Peer-Reviewed Original ResearchConceptsDRG neuronsAction potential durationMedium neuronsNerve injurySmall neuronsLarge neuronsAction potentialsGABA conductanceNerve ligationControl neuronsPotential durationAdult rat dorsal root ganglion neuronsGABAA receptor-mediated conductanceRat dorsal root ganglion neuronsWhole-cell patch-clamp recordingsCell patch-clamp recordingsDorsal root ganglion neuronsMean action potential durationShort-duration action potentialsControl DRG neuronsSciatic nerve ligationEffects of axotomyReceptor-mediated conductanceTraumatic nerve injuryPatch-clamp recordings
1994
Calcium signals in neurons
Rand M, Leinders-Zufall T, Agulian S, Kocsis J. Calcium signals in neurons. Nature 1994, 371: 291-292. PMID: 8090198, DOI: 10.1038/371291b0.Peer-Reviewed Original ResearchIntracellular calcium mobilization and neurite outgrowth in mammalian neurons
Kocsis J, Rand M, Lankford K, Waxman S. Intracellular calcium mobilization and neurite outgrowth in mammalian neurons. Developmental Neurobiology 1994, 25: 252-264. PMID: 8195789, DOI: 10.1002/neu.480250306.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCaffeineCalciumCalcium ChannelsCell CompartmentationCell DifferentiationCells, CulturedFemaleGanglia, SpinalGene Expression RegulationImage Processing, Computer-AssistedIntracellular FluidIon Channel GatingLasersMicroscopy, FluorescenceModels, BiologicalNeuritesNeuronsRatsRats, WistarTerpenesThapsigarginConceptsDepolarization-induced Ca2Calcium-induced calcium releaseDRG neuronsIntracellular Ca2Cultured adult rat dorsal root ganglion neuronsAdult rat dorsal root ganglion neuronsRat dorsal root ganglion neuronsNeurite outgrowthDorsal root ganglion neuronsIntact intracellular Ca2Intracellular calcium mobilizationIndicator dye fluo-3Nuclear Ca2Dye Fluo-3Endoplasmic reticulum Ca2Ganglion neuronsCalcium mobilizationDependent kinase IICalcium releaseFluo-3NeuronsMammalian neuronsReticulum Ca2Elevated Ca2Elicit neurite outgrowthChapter 14 Nuclear calcium elevation may initiate neurite outgrowth in mammalian neurons
Kocsis J, Rand M, Lankford K, Waxman S. Chapter 14 Nuclear calcium elevation may initiate neurite outgrowth in mammalian neurons. Progress In Brain Research 1994, 103: 137-151. PMID: 7886202, DOI: 10.1016/s0079-6123(08)61134-6.Peer-Reviewed Original ResearchNuclear and cytoplasmic Ca2+ signals in developing rat dorsal root ganglion neurons studied in excised tissue
Utzschneider D, Rand M, Waxman S, Kocsis J. Nuclear and cytoplasmic Ca2+ signals in developing rat dorsal root ganglion neurons studied in excised tissue. Brain Research 1994, 635: 231-237. PMID: 8173960, DOI: 10.1016/0006-8993(94)91444-3.Peer-Reviewed Original ResearchConceptsDorsal root ganglion neuronsEmbryonic DRG neuronsDepolarization-induced Ca2DRG neuronsGanglion neuronsRat dorsal root ganglion neuronsAdult dorsal root ganglion neuronsCytoplasmic Ca2Sensitive fluorescent dye fluo-3Rat DRG neuronsDorsal root gangliaExcised dorsal root gangliaFluorescent dye fluo-3Postnatal time periodsElevated extracellular potassiumEarly postnatal time periodsBrief electrical stimuliNuclear Ca2Dye Fluo-3Neonatal gangliaNeonatal neuronsTarget innervationRoot gangliaAdult neuronsEmbryonic neurons
1992
Mutual excitation among dorsal root ganglion neurons in the rat
Utzschneider D, Kocsis J, Devor M. Mutual excitation among dorsal root ganglion neurons in the rat. Neuroscience Letters 1992, 146: 53-56. PMID: 1475049, DOI: 10.1016/0304-3940(92)90170-c.Peer-Reviewed Original ResearchConceptsDorsal root gangliaDorsal rootsDorsal root ganglion neuronsPrimary sensory neuronsExcised dorsal root gangliaNerve injuryDRG neuronsGanglion neuronsMost neuronsRoot gangliaSensory abnormalitiesIntracellular recordingsDR stimulationRepetitive stimulationSensory neuronsTransient riseNeighboring neuronsNeuronsStimulationSimilar kineticsMutual excitationGangliaInjuryRatsAbnormalitiesIntranuclear Ca2+ transients during neurite regeneration of an adult mammalian neuron.
Birch B, Eng D, Kocsis J. Intranuclear Ca2+ transients during neurite regeneration of an adult mammalian neuron. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 7978-7982. PMID: 1518824, PMCID: PMC49838, DOI: 10.1073/pnas.89.17.7978.Peer-Reviewed Original ResearchConceptsNeurite extensionNuclear eventsMammalian dorsal root ganglion neuronsSubcellular calcium transientsIntranuclear Ca2Confocal laser scanning microscopyGene expressionMammalian neuronsAdult mammalian neuronsCytoplasmic levelsLaser scanning microscopyNuclear Ca2Importance of Ca2Activity-dependent modulationScanning microscopyNeurite regenerationDepolarization-induced increaseCa2Transmembrane Ca2Dorsal root ganglion neuronsRegenerationExtracellular Ca2CytoplasmNucleusNeurons