2018
The phenotypic and functional properties of mouse yolk-sac-derived embryonic macrophages
Yosef N, Vadakkan TJ, Park JH, Poché RA, Thomas JL, Dickinson ME. The phenotypic and functional properties of mouse yolk-sac-derived embryonic macrophages. Developmental Biology 2018, 442: 138-154. PMID: 30016639, PMCID: PMC6190604, DOI: 10.1016/j.ydbio.2018.07.009.Peer-Reviewed Original ResearchConceptsEmbryonic macrophagesErythro-myeloid progenitorsNeural stem/progenitor cellsExtra-embryonic yolk sacMouse neural stem/progenitor cellsEndothelial cell cord formationStem/progenitor cellsNon-immune functionsEC tube formationDevelopmental processesPrimitive erythroblastsTissue-resident populationsCord formationEarly cellsDirect cellKey inducerProgenitor cellsYolk sacCell contactEssential roleTube formationSpecialized propertiesBone marrowCell maturationProgenitors
2016
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres.
Lopez-Ramirez MA, Calvo CF, Ristori E, Thomas JL, Nicoli S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. Journal Of Visualized Experiments 2016, 53617. PMID: 26967835, PMCID: PMC4828210, DOI: 10.3791/53617.Peer-Reviewed Original ResearchConceptsAdult zebrafish brainStem/progenitor cellsZebrafish brainMolecular mechanismsRelevant model organismZebrafish adult brainProgenitor cellsSingle-cell dissociationSpecific signaling pathwaysAdult stem/progenitor cellsStem/progenitor cell proliferationNeural stem/progenitor cell proliferationProgenitor cell proliferationNeural stem/progenitor cellsModel organismsGene expressionCell dissociationSignaling pathwaysMultipotent neural stem/progenitor cellsNeurosphere assayAdult neurogenesisCell proliferationZebrafishBrain regenerationAdult brain
2015
Embryonic Stem Cells License a High Level of Dormant Origins to Protect the Genome against Replication Stress
Ge XQ, Han J, Cheng EC, Yamaguchi S, Shima N, Thomas JL, Lin H. Embryonic Stem Cells License a High Level of Dormant Origins to Protect the Genome against Replication Stress. Stem Cell Reports 2015, 5: 185-194. PMID: 26190528, PMCID: PMC4618655, DOI: 10.1016/j.stemcr.2015.06.002.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsStem/progenitor cellsNeural stem/progenitor cellsStem cellsProgenitor cellsTissue stem/progenitor cellsMCM2-7 complexDNA replication originsTissue-specific stem/progenitor cellsStem cell typesGenome integrityGenomic integrityReplication stressDormant originsReplication forksReplicative stressDNA replicationReplication originsNeural lineagesDNA damageS phaseCell typesAbnormal neurogenesisCellsGenomeVascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans
Han J, Calvo CF, Kang TH, Baker KL, Park JH, Parras C, Levittas M, Birba U, Pibouin-Fragner L, Fragner P, Bilguvar K, Duman RS, Nurmi H, Alitalo K, Eichmann AC, Thomas JL. Vascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans. Cell Reports 2015, 10: 1158-1172. PMID: 25704818, PMCID: PMC4685253, DOI: 10.1016/j.celrep.2015.01.049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell ProliferationCells, CulturedEmbryonic Stem CellsExtracellular Signal-Regulated MAP KinasesHippocampusHumansMiceMice, Inbred C57BLNeural Stem CellsNeurogenesisProto-Oncogene Proteins c-aktRecombinant ProteinsSignal TransductionVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-3ConceptsHuman embryonic stem cellsNeural stem cellsVascular endothelial growth factor receptor 3Growth factor receptor 3NSC activationStem cellsProgenitor cellsAdult hippocampal neural stem cellsEmbryonic stem cellsNeural stem cell activationStem cell activationQuiescent neural stem cellsNeural progenitor cellsCell fateReceptor 3Specific regulatorsAdult mammalian hippocampusMolecular mechanismsCell cycleHippocampal neural stem cellsLigand VEGFERK pathwayConditional deletionNew neuronsVEGFR3
2008
Genetic tracing of subpopulation neurons in the prethalamus of mice (Mus musculus)
Delaunay D, Heydon K, Miguez A, Schwab M, Nave K, Thomas J, Spassky N, Martinez S, Zalc B. Genetic tracing of subpopulation neurons in the prethalamus of mice (Mus musculus). The Journal Of Comparative Neurology 2008, 512: 74-83. PMID: 18973275, DOI: 10.1002/cne.21904.Peer-Reviewed Original ResearchConceptsVentral lateral geniculateCre/lox reporter systemGFP reporter miceGABAergic cellsGABAergic neuronsGlutamatergic neuronsLateral geniculateZona incertaHypothalamic nucleiSubthalamic nucleusReporter miceZona limitans intrathalamicaVentricular cellsIntergeniculate leafletNeuronsProgenitor cellsPosterior partGenetic tracingDiencephalonPool of progenitorsGenetic labelingPLP transcriptsMiceBasal plateCellsEarly Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells
Delaunay D, Heydon K, Cumano A, Schwab M, Thomas J, Suter U, Nave K, Zalc B, Spassky N. Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells. Journal Of Neuroscience 2008, 28: 2551-2562. PMID: 18322099, PMCID: PMC6671176, DOI: 10.1523/jneurosci.5497-07.2008.Peer-Reviewed Original ResearchConceptsGlial cellsEmbryonic neural stem cellsNeuronal progenitor cellsFate restrictionRadial glial cellsEmbryonic developmentNeural stem cellsNeuroepithelial progenitorsFate mappingNeuronal precursorsNeuroepithelial cellsNeurogenic periodStem cellsClonal analysisGlial precursorsProgenitor cellsGliogenic periodCellsProteolipid proteinNew poolDifferent time pointsLater stagesEmbryogenesis
2007
VEGFR‐3 and VEGF‐C: newcomers in the neurovascular cross‐talk
Thomas J. VEGFR‐3 and VEGF‐C: newcomers in the neurovascular cross‐talk. The FASEB Journal 2007, 21: a197-a197. DOI: 10.1096/fasebj.21.5.a197-a.Peer-Reviewed Original ResearchVEGFR-3Central nervous systemVEGF-CLymphangiogenic factors VEGF-CNervous systemVEGF-C/VEGFR-3Biology of neural stem cellsEmbryonic central nervous systemMigration of neural precursorsNeural stem cellsVEGF-C/DProgenitor cellsPostnatal neurogenesisAnimal modelsBrain tumorsPostmitotic neuronsStem cellsNeural precursorsNeural proliferationNeurogenic nicheGain of function experimentsNeural cellsCross-talk
2006
VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain
Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Bréant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Nature Neuroscience 2006, 9: 340-348. PMID: 16462734, DOI: 10.1038/nn1646.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCell DifferentiationCells, CulturedEvolution, MolecularIntermediate Filament ProteinsLarvaLateral VentriclesMiceMice, KnockoutMice, TransgenicNerve Growth FactorsNerve Tissue ProteinsNestinNeuronsOligodendrogliaOptic NerveRatsRats, WistarStem CellsVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-3Xenopus laevisConceptsNeural progenitor cellsReceptor VEGFR-3Mouse embryosNeural progenitorsVEGFR-3Progenitor cellsVertebrate embryonic brainBlood vessel defectsOligodendrocyte precursor cellsXenopus laevisAction of VEGFEmbryonic brainVascular endothelial growth factor CVEGF-C knockdownNeural cellsPrecursor cellsVessel defectsFactor CEmbryosGrowth factorProgenitorsCellsProliferation of OPCsVascular systemLymphatic vessels
2001
The Early Steps of Oligodendrogenesis: Insights from the Study of the plp Lineage in the Brain of Chicks and Rodents
Spassky N, Olivier C, Cobos I, LeBras B, Goujet-Zalc C, Martínez S, Zalc B, Thomas J. The Early Steps of Oligodendrogenesis: Insights from the Study of the plp Lineage in the Brain of Chicks and Rodents. Developmental Neuroscience 2001, 23: 318-326. PMID: 11756747, DOI: 10.1159/000048715.Peer-Reviewed Original ResearchConceptsOligodendroglial genesBrain of chicksCentral nervous systemMyelin-forming cellsSpinal cordPrimitive progenitor cellsNervous systemRodent brainOligodendroglial markersOligodendrocyte lineageEmbryonic brainProgenitor cellsBrainOligodendrogenesisCell lineagesProgenitor stageCellsChicksCordExpressionEarly stepsOligodendrocytesMonofocal origin of telencephalic oligodendrocytes in the anterior entopeduncular area of the chick embryo
Olivier C, Cobos I, Villegas E, Spassky N, Zalc B, Martinez S, Thomas J. Monofocal origin of telencephalic oligodendrocytes in the anterior entopeduncular area of the chick embryo. Development 2001, 128: 1757-1769. PMID: 11311157, DOI: 10.1242/dev.128.10.1757.Peer-Reviewed Original ResearchConceptsAnterior entopeduncular areaTelencephalic oligodendrocytesOligodendrocyte progenitor cellsCentral nervous systemMyelin-forming cellsRostrocaudal distributionCaudorostral axisVentricular originNervous systemOligodendroglial cellsChick brainOligodendrocytesOligodendrocyte precursorsOligodendrocyte progenitorsVentricular neuroepitheliumCaudal forebrainEntire telencephalonMigratory propertiesQuail-chick chimerasProgenitor cellsBrainChick embryosCellsOligodendrogenesis
2000
Spatiotemporal development of oligodendrocytes in the embryonic brain
Thomas J, Spassky N, Villegas E, Olivier C, Cobos I, Goujet‐Zalc C, Martínez S, Zalc B. Spatiotemporal development of oligodendrocytes in the embryonic brain. Journal Of Neuroscience Research 2000, 59: 471-476. PMID: 10679785, DOI: 10.1002/(sici)1097-4547(20000215)59:4<471::aid-jnr1>3.0.co;2-3.Peer-Reviewed Original ResearchConceptsCentral nervous systemOligodendrocyte precursor cellsPrecursor cellsNeural tubeSite of originSubventricular zoneNervous systemVentricular layerOligodendrocyte precursorsFirst neuronsOligodendrocyte progenitorsOligodendrocytesEmbryonic brainRostrocaudal axisLast cell typeQuail-chick chimerasProgenitor cellsRecent dataBrainCell typesMosaic populationProgenitor stageCellsNeurons