2017
Structure and Functional Analysis of ClbQ, an Unusual Intermediate-Releasing Thioesterase from the Colibactin Biosynthetic Pathway
Guntaka NS, Healy AR, Crawford JM, Herzon SB, Bruner SD. Structure and Functional Analysis of ClbQ, an Unusual Intermediate-Releasing Thioesterase from the Colibactin Biosynthetic Pathway. ACS Chemical Biology 2017, 12: 2598-2608. PMID: 28846367, PMCID: PMC5830302, DOI: 10.1021/acschembio.7b00479.Peer-Reviewed Original ResearchConceptsColibactin gene clusterÅ crystal structurePolyketide secondary metabolitesAcyl-thioester substratesColibactin biosynthesisGene clusterBiosynthetic pathwayAtypical roleGene productsBiochemical characterizationFunctional analysisSecondary metabolitesGreater catalytic efficiencyCancer formationColorectal cancer formationHuman gutSpecific functionsNovel insightsCarrier proteinThioesteraseGenetic deletionClbQColibactinCatalytic efficiencyPathway
2012
Small molecule perimeter defense in entomopathogenic bacteria
Crawford JM, Portmann C, Zhang X, Roeffaers MB, Clardy J. Small molecule perimeter defense in entomopathogenic bacteria. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 10821-10826. PMID: 22711807, PMCID: PMC3390839, DOI: 10.1073/pnas.1201160109.Peer-Reviewed Original ResearchConceptsBacterial cell surfaceConfocal fluorescence microscopyCell surfacePhysical associationHuman pathogen Vibrio choleraeInsect innate immune systemBiochemical assaysInnate immune systemPathogen Vibrio choleraeFluorescence microscopyPrecursor-directed biosynthesisE. coli cellsInsect pathogensPathway mutantsEntomopathogenic bacteriaHeterologous expressionXenorhabdus nematophilaColi cellsEscherichia coliVibrio choleraeClass of moleculesBioorthogonal probesEffective inhibitor concentrationBiosynthesisHost defense
2011
Dihydrophenylalanine: A Prephenate-Derived Photorhabdus luminescens Antibiotic and Intermediate in Dihydrostilbene Biosynthesis
Crawford JM, Mahlstedt SA, Malcolmson SJ, Clardy J, Walsh CT. Dihydrophenylalanine: A Prephenate-Derived Photorhabdus luminescens Antibiotic and Intermediate in Dihydrostilbene Biosynthesis. Cell Chemical Biology 2011, 18: 1102-1112. PMID: 21944749, PMCID: PMC3183431, DOI: 10.1016/j.chembiol.2011.07.009.Peer-Reviewed Original Research
2010
Exploiting a Global Regulator for Small Molecule Discovery in Photorhabdus luminescens
Kontnik R, Crawford JM, Clardy J. Exploiting a Global Regulator for Small Molecule Discovery in Photorhabdus luminescens. ACS Chemical Biology 2010, 5: 659-665. PMID: 20524642, PMCID: PMC2912427, DOI: 10.1021/cb100117k.Peer-Reviewed Original ResearchConceptsGlobal transcriptional regulatorSmall moleculesSecondary metabolite productionGenes/lociTight regulatory controlUseful biological probesSmall molecule discoverySuch small moleculesGlobal regulatorBacterial genomesFunctional diversityInsect larvaeTranscriptional regulatorsTranscriptional repressorComplex symbiosisNematode wormsMetabolite productionMolecule discoveryPhotorhabdusMutantsDramatic upregulationSymbiosisRegulatory controlRemarkable rangeRegulator
2008
Acyl‐Carrier Protein–Phosphopantetheinyltransferase Partnerships in Fungal Fatty Acid Synthases
Crawford JM, Vagstad AL, Ehrlich KC, Udwary DW, Townsend CA. Acyl‐Carrier Protein–Phosphopantetheinyltransferase Partnerships in Fungal Fatty Acid Synthases. ChemBioChem 2008, 9: 1559-1563. PMID: 18551496, PMCID: PMC3189688, DOI: 10.1002/cbic.200700659.Peer-Reviewed Original Research