2018
Functional Characterization of a Condensation Domain That Links Nonribosomal Peptide and Pteridine Biosynthetic Machineries in Photorhabdus luminescens
Perez CE, Park HB, Crawford JM. Functional Characterization of a Condensation Domain That Links Nonribosomal Peptide and Pteridine Biosynthetic Machineries in Photorhabdus luminescens. Biochemistry 2018, 57: 354-361. PMID: 29111689, DOI: 10.1021/acs.biochem.7b00863.Peer-Reviewed Original ResearchMeSH KeywordsChromatography, LiquidGenes, BacterialMass SpectrometryMultigene FamilyPeptide BiosynthesisPeptide SynthasesPhotorhabdusPteridinesConceptsNonribosomal peptide synthetasesCondensation domainMetabolic pathwaysSecondary metabolite biosynthesisSpecialized metabolic pathwaysBiosynthetic gene clusterNRPS condensation domainDistinct enzymatic systemsImportant small moleculesSecondary metabolite analysisCitric acid cycleMetabolite biosynthesisFunctional diversityGene clusterBiosynthetic machineryCellular redoxPeptide synthetasesNonribosomal peptidesBiosynthetic systemsQuorum sensingFunctional characterizationSolution studiesGenetic lociNatural productsBiochemical level
2016
Pyrazinone protease inhibitor metabolites from Photorhabdus luminescens
Park HB, Crawford JM. Pyrazinone protease inhibitor metabolites from Photorhabdus luminescens. The Journal Of Antibiotics 2016, 69: 616-621. PMID: 27353165, PMCID: PMC5003743, DOI: 10.1038/ja.2016.79.Peer-Reviewed Original ResearchConceptsSpecialized metabolitesNew specialized metabolitesMutualistic associationAmino acid levelsInsect pathogenesisInsect preyGalleria mellonella larvaePhotorhabdus genusP. luminescensPhenotypic variationFamilies HeterorhabditidaeNematode developmentEntomopathogenic bacteriumProtease inhibitory activityFree amino acid levelsRich mediumAmino acidsMellonella larvaePhenotypic variantsMarfey's analysisPhotorhabdusCirculatory fluidCulture extractsMetabolitesHeterorhabditidaeConvergent and Modular Synthesis of Candidate Precolibactins. Structural Revision of Precolibactin A
Healy AR, Vizcaino MI, Crawford JM, Herzon SB. Convergent and Modular Synthesis of Candidate Precolibactins. Structural Revision of Precolibactin A. Journal Of The American Chemical Society 2016, 138: 5426-5432. PMID: 27025153, PMCID: PMC5049697, DOI: 10.1021/jacs.6b02276.Peer-Reviewed Original Research
2010
Siderophores from Neighboring Organisms Promote the Growth of Uncultured Bacteria
D'Onofrio A, Crawford JM, Stewart EJ, Witt K, Gavrish E, Epstein S, Clardy J, Lewis K. Siderophores from Neighboring Organisms Promote the Growth of Uncultured Bacteria. Cell Chemical Biology 2010, 17: 254-264. PMID: 20338517, PMCID: PMC2895992, DOI: 10.1016/j.chembiol.2010.02.010.Peer-Reviewed Original ResearchConceptsCulturing of organismsUncultured isolatesUncultured bacteriaGrowth factorGrowth promotionCultured microbesCommunity establishmentBacterial speciesSediment biofilmHelper strainSiderophoresCultured organismsOrganismsLack of growthBacteriaPetri dishesSynthetic mediumSame environmentHabitatsIsolatesMicrobesSpeciesGrowthStrainsPromiscuity
2008
Deconstruction of Iterative Multidomain Polyketide Synthase Function
Crawford J, Thomas P, Scheerer J, Vagstad A, Kelleher N, Townsend C. Deconstruction of Iterative Multidomain Polyketide Synthase Function. Science 2008, 320: 243-246. PMID: 18403714, PMCID: PMC2480491, DOI: 10.1126/science.1154711.Peer-Reviewed Original ResearchConceptsIterative polyketide synthasesIndividual catalytic domainsProduct template (PT) domainsPolyketide synthasesStarter unitCatalytic domainAromatic polyketidesBiosynthetic enzymesTemplate domainSynthase functionCarcinogen aflatoxin B1PksAMechanistic featuresEnzymePt domainsKetosynthaseDomainPolyketidesBiosynthesisSynthasesCascadeReconstitutionAccumulationFamily