2018
Functional Characterization of a Condensation Domain That Links Nonribosomal Peptide and Pteridine Biosynthetic Machineries in Photorhabdus luminescens
Perez CE, Park HB, Crawford JM. Functional Characterization of a Condensation Domain That Links Nonribosomal Peptide and Pteridine Biosynthetic Machineries in Photorhabdus luminescens. Biochemistry 2018, 57: 354-361. PMID: 29111689, DOI: 10.1021/acs.biochem.7b00863.Peer-Reviewed Original ResearchConceptsNonribosomal peptide synthetasesCondensation domainMetabolic pathwaysSecondary metabolite biosynthesisSpecialized metabolic pathwaysBiosynthetic gene clusterNRPS condensation domainDistinct enzymatic systemsImportant small moleculesSecondary metabolite analysisCitric acid cycleMetabolite biosynthesisFunctional diversityGene clusterBiosynthetic machineryCellular redoxPeptide synthetasesNonribosomal peptidesBiosynthetic systemsQuorum sensingFunctional characterizationSolution studiesGenetic lociNatural productsBiochemical level
2017
Domain-Targeted Metabolomics Delineates the Heterocycle Assembly Steps of Colibactin Biosynthesis
Trautman EP, Healy AR, Shine EE, Herzon SB, Crawford JM. Domain-Targeted Metabolomics Delineates the Heterocycle Assembly Steps of Colibactin Biosynthesis. Journal Of The American Chemical Society 2017, 139: 4195-4201. PMID: 28240912, PMCID: PMC5831107, DOI: 10.1021/jacs.7b00659.Peer-Reviewed Original ResearchConceptsNonribosomal peptide synthetasesPolyketide synthasesModular polyketide synthasesSite-directed mutagenesisCombination of genesCellular metabolic levelsColibactin biosynthesisMultidomain proteinsProtein domainsPeptide synthetasesCatalytic domainControl pathwaysProtein biochemicalPathway analysisCertain Escherichia coliComplete deletionHybrid pathwayEscherichia coliFunctional readoutBiosynthesisCatalytic mechanismCellular levelMetabolic levelPathwayGenes