2000
DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON
Comer J, Nicholson W, Paddock C, Sumner J, Childs J. DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON. Journal Of Wildlife Diseases 2000, 36: 705-712. PMID: 11085432, DOI: 10.7589/0090-3558-36.4.705.Peer-Reviewed Original Research
1999
Outcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance
Childs J, Sumner J, Nicholson W, Massung R, Standaert S, Paddock C. Outcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance. Journal Of Clinical Microbiology 1999, 37: 2997-3000. PMID: 10449489, PMCID: PMC85432, DOI: 10.1128/jcm.37.9.2997-3000.1999.Peer-Reviewed Original ResearchConceptsHuman monocytic ehrlichiosisDiagnosis of HMEConvalescent-phase serum samplesMonocytic ehrlichiosisDetection of morulaeInitial clinical presentationCulture-proven casesE. chaffeensis antigenWhole blood specimensCompatible illnessClinical presentationInitial presentationSerologic responseCase definitionHME patientsBlood specimensImmunoglobulin MPatientsBlood smearsIndirect immunofluorescenceEhrlichial DNADiagnostic testsSerum samplesProbable diseaseEhrlichia chaffeensisHidden Mortality Attributable to Rocky Mountain Spotted Fever: Immunohistochemical Detection of Fatal, Serologically Unconfirmed Disease
Paddock C, Greer P, Ferebee T, Singleton J, McKechnie D, Treadwell T, Krebs J, Clarke M, Holman R, Olson J, Childs J, Zaki S. Hidden Mortality Attributable to Rocky Mountain Spotted Fever: Immunohistochemical Detection of Fatal, Serologically Unconfirmed Disease. The Journal Of Infectious Diseases 1999, 179: 1469-1476. PMID: 10228069, DOI: 10.1086/314776.Peer-Reviewed Original ResearchConceptsCase fatality ratioDiagnosis of RMSFIHC stainingRocky Mountain Spotted FeverLaboratory-confirmed casesAcute-phase seraTickborne infectionHidden mortalityIgM antibodiesDiagnostic titerPolymerase chain reactionIHC findingsPatient seraSerologic assaysImmunohistochemical stainingSpotted FeverImmunohistochemical detectionFatal diseasePatientsDisease controlIndirect immunofluorescenceNotifiable diseaseDiseaseUnderestimates of mortalityTissue samplesSerologic Testing for Human Granulocytic Ehrlichiosis at a National Referral Center
Comer J, Nicholson W, Olson J, Childs J. Serologic Testing for Human Granulocytic Ehrlichiosis at a National Referral Center. Journal Of Clinical Microbiology 1999, 37: 558-564. PMID: 9986812, PMCID: PMC84468, DOI: 10.1128/jcm.37.3.558-564.1999.Peer-Reviewed Original ResearchConceptsHGE agentSerum samplesNational referral centerE. chaffeensis antigenSpecific etiologic agentState health departmentsE. chaffeensisHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisHuman monocytic ehrlichiosisReferral centerSerologic testingEhrlichial antigenAverage ageHealth departmentsHuman ehrlichiosisPatientsEtiologic agentProbable casesHigh titersIll individualsGranulocytic ehrlichiosisDisease controlIndirect immunofluorescenceMonocytic ehrlichiosis
1998
Retrospective Serosurvey for Human Granulocytic Ehrlichiosis Agent in Urban White-footed Mice from Maryland
Bunnell J, Stephen D, Childs J, Glass G. Retrospective Serosurvey for Human Granulocytic Ehrlichiosis Agent in Urban White-footed Mice from Maryland. Journal Of Wildlife Diseases 1998, 34: 179-181. PMID: 9476244, DOI: 10.7589/0090-3558-34.1.179.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosis (HGE) agentIndirect immunofluorescenceHGE agentEhrlichia equiSerum samplesRetrospective serosurveyBaltimore City
1997
An indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis
Nicholson W, Comer J, Sumner J, Gingrich-Baker C, Coughlin R, Magnarelli L, Olson J, Childs J. An indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 1510-1516. PMID: 9163471, PMCID: PMC229776, DOI: 10.1128/jcm.35.6.1510-1516.1997.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisCell culture-derived antigenHuman antibodiesGranulocytic ehrlichiosisIndirect immunofluorescenceHGE agentAnimal-derived antigensDetection of antibodiesTick-derived isolatesPromyelocytic leukemia cell lineAntigen presentationHuman promyelocytic leukemia cell lineHealthy individualsLeukemia cell linesHGE patientsSera reactiveEhrlichia phagocytophilaHigh titersAntigenImmunofluorescence methodE. equiEhrlichia equiRoutine testingAntibodiesSpecific antibodies
1994
Isolation of the causative agent of hantavirus pulmonary syndrome.
Elliott L, Ksiazek T, Rollin P, Spiropoulou C, Morzunov S, Monroe M, Goldsmith C, Humphrey C, Zaki S, Krebs J, Maupin G, Gage K, Childs J, Nichol S, Peters C. Isolation of the causative agent of hantavirus pulmonary syndrome. American Journal Of Tropical Medicine And Hygiene 1994, 51: 102-8. PMID: 8059907, DOI: 10.4269/ajtmh.1994.51.102.Peer-Reviewed Original ResearchConceptsHantavirus pulmonary syndromePulmonary syndromeAcute respiratory illnessReverse transcriptase-polymerase chain reactionCausative agentTypical morphologic featuresConvalescent-phase seraTranscriptase-polymerase chain reactionUnknown hantavirusRespiratory illnessLung tissueHPS patientsVirus antigenEtiologic agentHigh mortalityMorphologic featuresIndirect immunofluorescenceGenus HantavirusVero cellsInfected cellsChain reactionRecent outbreakCell linesSyndromeDeer mice