2000
Molecular identification and functional characterization of Mdr1a in rat cholangiocytes
Gigliozzi A, Fraioli F, Sundaram P, Lee J, Mennone A, Alvaro D, Boyer J. Molecular identification and functional characterization of Mdr1a in rat cholangiocytes. Gastroenterology 2000, 119: 1113-1122. PMID: 11040198, DOI: 10.1053/gast.2000.18156.Peer-Reviewed Original ResearchMeSH KeywordsAcridinesAnimalsATP Binding Cassette Transporter, Subfamily BATP Binding Cassette Transporter, Subfamily B, Member 1ATP-Binding Cassette TransportersBile DuctsCell DivisionCell MembraneCytosolDrug Resistance, MultipleIn Vitro TechniquesIsoquinolinesKineticsMaleMicroscopy, ConfocalRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionRhodamine 123RNA, MessengerTetrahydroisoquinolinesTranscription, GeneticVanadatesVerapamilConceptsCholangiocyte cell linePlasma membrane proteinsRat cholangiocyte cell lineCell linesCytosolic domainMembrane proteinsRhodamine 123Functional characterizationGene productsRNA transcriptsMolecular identificationMembrane subfractionsPair fragmentWestern blot analysisWestern analysisApical membraneFunctional assaysFunctional evidenceBlot analysisConfocal microscopyP-glycoprotein inhibitor verapamilRat cholangiocytesLipophilic xenobioticsReverse transcription-polymerase chain reactionDose-dependent manner
1991
Mechanism of mercurial inhibition of sodium-coupled alanine uptake in liver plasma membrane vesicles from Raja erinacea
Sellinger M, Ballatori N, Boyer J. Mechanism of mercurial inhibition of sodium-coupled alanine uptake in liver plasma membrane vesicles from Raja erinacea. Toxicology And Applied Pharmacology 1991, 107: 369-376. PMID: 1994517, DOI: 10.1016/0041-008x(91)90216-2.Peer-Reviewed Original ResearchConceptsDependent alanine uptakePlasma membrane vesiclesMembrane vesiclesAlanine uptakePrimitive vertebratesPlasma membraneTransport proteinsConcentration-dependent mechanismLiver plasma membrane vesiclesMicroM HgCl2Mammalian hepatocytesAlanine carrierSulfhydryl groupsLittle skateMembrane integrityRaja erinaceaDirect interactionVesiclesVesicle integrityMembrane permeabilityMercurial inhibitionUptake rateDose-dependent mannerHgCl2 concentrationsLiver plasma membranes