2007
Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase
Fretz JA, Shevde NK, Singh S, Darnay BG, Pike JW. Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase. Endocrinology 2007, 22: 737-750. PMID: 18063694, PMCID: PMC2262172, DOI: 10.1210/me.2007-0333.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAnimalsBlotting, WesternBone and BonesCell LineChromatin ImmunoprecipitationHomeostasisIsoenzymesMiceMice, Inbred C57BLNFATC Transcription FactorsOsteoclastsPromoter Regions, GeneticRANK LigandReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTartrate-Resistant Acid PhosphataseTranscription, GeneticUp-RegulationConceptsNFAT membersRNA polymerase IIDNA-binding proteinsSpecific transcription factorsChromatin immunoprecipitation analysisSignal transduction pathwaysAdditional molecular detailsNuclear factorActivated T cells cytoplasmic 1Polymerase IIAcp5 promotersTranscription factorsTransduction pathwaysMolecular detailsTarget genesOwn expressionImmunoprecipitation analysisP1 promoterBone-resorbing cellsReceptor activatorHematopoietic precursorsGenesNuclear factor-κB ligandCytoplasmic 1Time-dependent accumulation1,25-Dihydroxyvitamin D3 induces expression of the Wnt signaling co-regulator LRP5 via regulatory elements located significantly downstream of the gene's transcriptional start site
Fretz JA, Zella LA, Kim S, Shevde NK, Pike JW. 1,25-Dihydroxyvitamin D3 induces expression of the Wnt signaling co-regulator LRP5 via regulatory elements located significantly downstream of the gene's transcriptional start site. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 440-445. PMID: 17229572, PMCID: PMC1868540, DOI: 10.1016/j.jsbmb.2006.11.018.Peer-Reviewed Original ResearchConceptsTranscriptional start siteGene transcriptional start siteChIP-chip analysisLRP5/LRP6Chromatin structureHuman genomeRegulatory regionsStart siteRegulatory elementsWnt familyHeterologous promoterCanonical WntMembrane receptorsMRNA transcriptsWntSecreted memberOsteoblastic cellsLRP5GenesVDRELRP5 geneBone formationProteinLrp5 locusOsteoblast numberMultiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3
Kim S, Yamazaki M, Zella LA, Meyer MB, Fretz JA, Shevde NK, Pike JW. Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 430-434. PMID: 17197168, PMCID: PMC1892901, DOI: 10.1016/j.jsbmb.2006.12.020.Peer-Reviewed Original ResearchConceptsTranscription start siteStart siteChip analysisMultiple enhancer regionsRNA polymerase IIPotential regulatory regionsTranscriptional start siteRANKL geneVDR/RXRChromatin hubHistone modificationsTranscriptional outputPolymerase IIRANKL gene expressionImportant functional consequencesMultiple enhancersRegulatory regionsTranscription factorsHeterologous promoterGene locusEnhancer regionGene expressionKb upstreamRANKL promoterLuciferase assay
2006
1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription.
Fretz JA, Zella LA, Kim S, Shevde NK, Pike JW. 1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription. Endocrinology 2006, 20: 2215-30. PMID: 16613987, DOI: 10.1210/me.2006-0102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesCells, CulturedChromatinChromatin ImmunoprecipitationConserved SequenceDimerizationDNAGene Expression RegulationHumansIntronsLDL-Receptor Related ProteinsLow Density Lipoprotein Receptor-Related Protein-5MiceMice, Inbred C57BLMolecular Sequence DataOsteoblastsPromoter Regions, GeneticProtein BindingReceptors, CalcitriolRetinoid X ReceptorsRNA Polymerase IISequence AlignmentTranscription, GeneticVitamin DVitamin D Response ElementConceptsVitamin D response elementD response elementResponse elementDNA microarray analysisChromatin structureChromatin immunoprecipitationOsteoblast biologyReceptor FrizzledHuman genomeLipoprotein receptor-related protein 5Regulatory regionsStart siteLrp5 locusLow-density lipoprotein receptor-related protein 5Sequence elementsHeterologous promoterMature osteoblastsMicroarray analysisOsteoblast precursorsDirect targetProtein 5Cell line originLine originPrimary osteoblastsGenes