Molecular Actions of 1,25‐Dihydroxyvitamin D3 on Genes Involved in Calcium Homeostasis*
Pike JW, Zella LA, Meyer MB, Fretz JA, Kim S. Molecular Actions of 1,25‐Dihydroxyvitamin D3 on Genes Involved in Calcium Homeostasis*. Journal Of Bone And Mineral Research 2007, 22: v16-v19. PMID: 18290714, DOI: 10.1359/jbmr.07s207.Peer-Reviewed Original ResearchConceptsTranscriptional start siteRegulatory regionsExpression of TRPV6RANKL geneKey regulatory regionsVertebrate organismsNovel genesChromatin immunoprecipitationStart siteRegulatory elementsUpstream sequencesGene expressionEnhancer regionMolecular mechanismsHuman TRPV6Upstream regionGenesTRPV6 geneMolecular actionsMolecular techniquesPrimary regulatorMicroarray methodKbTRPV6Expression1,25-Dihydroxyvitamin D3 induces expression of the Wnt signaling co-regulator LRP5 via regulatory elements located significantly downstream of the gene's transcriptional start site
Fretz JA, Zella LA, Kim S, Shevde NK, Pike JW. 1,25-Dihydroxyvitamin D3 induces expression of the Wnt signaling co-regulator LRP5 via regulatory elements located significantly downstream of the gene's transcriptional start site. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 440-445. PMID: 17229572, PMCID: PMC1868540, DOI: 10.1016/j.jsbmb.2006.11.018.Peer-Reviewed Original ResearchConceptsTranscriptional start siteGene transcriptional start siteChIP-chip analysisLRP5/LRP6Chromatin structureHuman genomeRegulatory regionsStart siteRegulatory elementsWnt familyHeterologous promoterCanonical WntMembrane receptorsMRNA transcriptsWntSecreted memberOsteoblastic cellsLRP5GenesVDRELRP5 geneBone formationProteinLrp5 locusOsteoblast numberMultiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3
Kim S, Yamazaki M, Zella LA, Meyer MB, Fretz JA, Shevde NK, Pike JW. Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 430-434. PMID: 17197168, PMCID: PMC1892901, DOI: 10.1016/j.jsbmb.2006.12.020.Peer-Reviewed Original ResearchConceptsTranscription start siteStart siteChip analysisMultiple enhancer regionsRNA polymerase IIPotential regulatory regionsTranscriptional start siteRANKL geneVDR/RXRChromatin hubHistone modificationsTranscriptional outputPolymerase IIRANKL gene expressionImportant functional consequencesMultiple enhancersRegulatory regionsTranscription factorsHeterologous promoterGene locusEnhancer regionGene expressionKb upstreamRANKL promoterLuciferase assay