Featured Publications
Deletion of the transcription factor EBF1 in perivascular stroma disrupts skeletal homeostasis and precipitates premature aging of the marrow microenvironment
Nelson T, Tommasini S, Fretz J. Deletion of the transcription factor EBF1 in perivascular stroma disrupts skeletal homeostasis and precipitates premature aging of the marrow microenvironment. Bone 2024, 187: 117198. PMID: 39002837, PMCID: PMC11410106, DOI: 10.1016/j.bone.2024.117198.Peer-Reviewed Original ResearchEarly B cell factor 1Disrupt cytokine signallingDisrupt BMP signalingTranscription factor early B cell factor 1B cell factor 1Mesenchymal lineagesGlucocorticoid receptor expressionImpaired osteogenic differentiationLineages in vitroTranscription factorsEBF1 deletionsProgenitor mobilizationMyeloid skewingImpaired fracture repairCytokine signalingSmad1 phosphorylationAge-dependent lossBMP signalingReceptor expressionMarrow microenvironmentAdipogenic lineages in vitroHematopoietic nicheMultiple lineagesBone marrowB cells
2013
Early B-cell factor 1 is an essential transcription factor for postnatal glomerular maturation
Fretz JA, Nelson T, Velazquez H, Xi Y, Moeckel GW, Horowitz MC. Early B-cell factor 1 is an essential transcription factor for postnatal glomerular maturation. Kidney International 2013, 85: 1091-1102. PMID: 24172684, PMCID: PMC4006322, DOI: 10.1038/ki.2013.433.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAlbuminuriaAnimalsBlood Urea NitrogenCell DifferentiationCells, CulturedGene Expression Regulation, DevelopmentalGenotypeGlomerular Filtration RateKidney GlomerulusMice, 129 StrainMice, Inbred C57BLMice, KnockoutOrganogenesisPhenotypePodocytesSignal TransductionTime FactorsTrans-ActivatorsVascular Endothelial Growth Factor AConceptsEarly B-cell factor 1B cell factor 1Transcription factorsNovel roleTranscription factor early B cell factor 1Expression of Ebf1Essential transcription factorFactor 1Podocyte maturationMesenchymal progenitorsB cell maturationProper maturationBlood urea nitrogen levelsElevated blood urea nitrogen levelsWild-type control miceGlomerular filtration rateVascular endothelial growth factor AGlomerular maturationUrea nitrogen levelsGrowth factor ARenal developmentEBF1Factor AGlomerular developmentNephron maturation
2010
How B cells influence bone biology in health and disease
Horowitz MC, Fretz JA, Lorenzo JA. How B cells influence bone biology in health and disease. Bone 2010, 47: 472-479. PMID: 20601290, PMCID: PMC2941392, DOI: 10.1016/j.bone.2010.06.011.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHematopoietic stem cellsB cell differentiationCell differentiationTranscription factorsBone cellsImportant regulatory interactionsBone phenotypeRegulatory interactionsB cellsTemporal mannerSupportive nicheStem cellsAdaptive immune systemBone biologyDifferentiationRANKL-RANKActivated B cellsEndosteal bone surfaceNormal bone remodelingNicheSpecific responsesPhenotypeCellsImportant roleImmune system
2008
Ebf1-dependent control of the osteoblast and adipocyte lineages
Hesslein DG, Fretz JA, Xi Y, Nelson T, Zhou S, Lorenzo JA, Schatz DG, Horowitz MC. Ebf1-dependent control of the osteoblast and adipocyte lineages. Bone 2008, 44: 537-546. PMID: 19130908, PMCID: PMC2657874, DOI: 10.1016/j.bone.2008.11.021.Peer-Reviewed Original ResearchConceptsNumber of osteoclastsBone formation parametersBone formation rateAdipocyte lineageBone marrow cellsOlfactory sensory neuronsSerum osteocalcinOsteoid volumeSensory neuronsAdipocyte numberBone marrowOsteoclast developmentMutant miceMarrow cellsMiceSubcutaneous sitesBone formationAdipocyte developmentStriking increaseDecreased depositionTranscription factorsOsteoblastsB cell fate specificationEBF1Adiposity
2007
Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase
Fretz JA, Shevde NK, Singh S, Darnay BG, Pike JW. Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase. Endocrinology 2007, 22: 737-750. PMID: 18063694, PMCID: PMC2262172, DOI: 10.1210/me.2007-0333.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAnimalsBlotting, WesternBone and BonesCell LineChromatin ImmunoprecipitationHomeostasisIsoenzymesMiceMice, Inbred C57BLNFATC Transcription FactorsOsteoclastsPromoter Regions, GeneticRANK LigandReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTartrate-Resistant Acid PhosphataseTranscription, GeneticUp-RegulationConceptsNFAT membersRNA polymerase IIDNA-binding proteinsSpecific transcription factorsChromatin immunoprecipitation analysisSignal transduction pathwaysAdditional molecular detailsNuclear factorActivated T cells cytoplasmic 1Polymerase IIAcp5 promotersTranscription factorsTransduction pathwaysMolecular detailsTarget genesOwn expressionImmunoprecipitation analysisP1 promoterBone-resorbing cellsReceptor activatorHematopoietic precursorsGenesNuclear factor-κB ligandCytoplasmic 1Time-dependent accumulationMultiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3
Kim S, Yamazaki M, Zella LA, Meyer MB, Fretz JA, Shevde NK, Pike JW. Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 430-434. PMID: 17197168, PMCID: PMC1892901, DOI: 10.1016/j.jsbmb.2006.12.020.Peer-Reviewed Original ResearchConceptsTranscription start siteStart siteChip analysisMultiple enhancer regionsRNA polymerase IIPotential regulatory regionsTranscriptional start siteRANKL geneVDR/RXRChromatin hubHistone modificationsTranscriptional outputPolymerase IIRANKL gene expressionImportant functional consequencesMultiple enhancersRegulatory regionsTranscription factorsHeterologous promoterGene locusEnhancer regionGene expressionKb upstreamRANKL promoterLuciferase assay