Kinetics of CO Insertion and Acetyl Group Transfer Steps, and a Model of the Acetyl-CoA Synthase Catalytic Mechanism
Tan X, Surovtsev I, Lindahl P. Kinetics of CO Insertion and Acetyl Group Transfer Steps, and a Model of the Acetyl-CoA Synthase Catalytic Mechanism. Journal Of The American Chemical Society 2006, 128: 12331-12338. PMID: 16967985, PMCID: PMC2527582, DOI: 10.1021/ja0627702.Peer-Reviewed Original ResearchMeSH KeywordsAcetate-CoA LigaseAcetylationAldehyde OxidoreductasesBacteria, AnaerobicCarbon MonoxideCatalysisKineticsMultienzyme ComplexesNickelConceptsCO insertionCatalytic mechanismGroup transferNiFeC EPR signalAcetyl-CoA synthase/carbon monoxide dehydrogenaseEquilibrium constantsMethyl groupSynthase/carbon monoxide dehydrogenaseCO insertion stepInsertion of COCarbon monoxide dehydrogenaseSubsequent catalytic stepsActive enzyme moleculesStopped-flow methodTransfer stepIndividual equilibrium constantsInsertion stepMethyl group transferEPR signalEnzyme moleculesCatalytic stepSimple kinetic modelSulfur proteinVariation of activityRate coefficients