2021
Efferent neurons control hearing sensitivity and protect hearing from noise through the regulation of gap junctions between cochlear supporting cells
Zhao H, Liu L, Yu N, Zhu Y, Mei L, Chen J, Liang C. Efferent neurons control hearing sensitivity and protect hearing from noise through the regulation of gap junctions between cochlear supporting cells. Journal Of Neurophysiology 2021, 127: 313-327. PMID: 34907797, PMCID: PMC8759971, DOI: 10.1152/jn.00468.2021.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCochlear NerveEfferent PathwaysFemaleGuinea PigsHair Cells, Auditory, OuterHearing Loss, Noise-InducedMaleNeurons, EfferentConceptsOuter hair cellsActive cochlear amplificationCochlear efferent systemDistortion product otoacoustic emissionsEfferent systemEfferent pathwaysHearing sensitivityMedial olivocochlear efferent fibersPresynaptic vesicular acetylcholine transportersGap junctionsOlivocochlear efferent fibersHair cellsApplication of acetylcholineCochlear supporting cellsProtection of hearingVesicular acetylcholine transporterActive cochlear amplifierCochlear amplificationPostsynaptic ACh receptorsProduct otoacoustic emissionsMOC efferent systemHair cell activityEfferent nervesEfferent inhibitionEfferent fibers
2014
Connexin26 (GJB2) deficiency reduces active cochlear amplification leading to late-onset hearing loss
Zhu Y, Chen J, Liang C, Zong L, Chen J, Jones R, Zhao H. Connexin26 (GJB2) deficiency reduces active cochlear amplification leading to late-onset hearing loss. Neuroscience 2014, 284: 719-729. PMID: 25451287, PMCID: PMC4268423, DOI: 10.1016/j.neuroscience.2014.10.061.Peer-Reviewed Original ResearchConceptsLate-onset hearing lossActive cochlear amplificationDistortion product otoacoustic emissionsHearing lossNonsyndromic hearing lossTherapeutic interventionsProgressive hearing lossHair cell lossPostnatal day 5Cochlear amplificationProduct otoacoustic emissionsConditional knockout miceKnockout miceClinical observationsDay 5Cell lossEndocochlear potentialOtoacoustic emissionsNormal hearingCx26 expressionDeafness mechanismMiceCx26 deficiencyCochleaIntervention
2013
Active cochlear amplification is dependent on supporting cell gap junctions
Zhu Y, Liang C, Chen J, Zong L, Chen G, Zhao H. Active cochlear amplification is dependent on supporting cell gap junctions. Nature Communications 2013, 4: 1786. PMID: 23653198, PMCID: PMC3675877, DOI: 10.1038/ncomms2806.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAuditory ThresholdCochlear Microphonic PotentialsConnexin 26ConnexinsEvoked Potentials, Auditory, Brain StemGap JunctionsGene DeletionGene TargetingHair Cells, Auditory, OuterHearing LossLabyrinth Supporting CellsMiceMice, KnockoutMolecular Motor ProteinsNonlinear DynamicsOtoacoustic Emissions, SpontaneousSpiral GanglionConceptsActive cochlear amplificationOuter hair cellsCell gap junctionsHearing lossCochlear amplificationHair cellsGap junctionsDistortion product otoacoustic emissionsOuter hair cell electromotilityHair cell electromotilitySevere hearing lossProduct otoacoustic emissionsShorter outer hair cellsHair-bundle movementsOuter pillar cellsLeftward shiftOtoacoustic emissionsAcoustic stimulationDeiters' cellsHearing sensitivityConnexin 26Active cochlear mechanicsNovel findingsPillar cellsBundle movement
2009
Modulation of Outer Hair Cell Electromotility by Cochlear Supporting Cells and Gap Junctions
Yu N, Zhao H. Modulation of Outer Hair Cell Electromotility by Cochlear Supporting Cells and Gap Junctions. PLOS ONE 2009, 4: e7923. PMID: 19936276, PMCID: PMC2775161, DOI: 10.1371/journal.pone.0007923.Peer-Reviewed Original Research
2008
Prestin up-regulation in chronic salicylate (aspirin) administration: An implication of functional dependence of prestin expression
Yu N, Zhu M, Johnson B, Liu Y, Jones R, Zhao H. Prestin up-regulation in chronic salicylate (aspirin) administration: An implication of functional dependence of prestin expression. Cellular And Molecular Life Sciences 2008, 65: 2407-2418. PMID: 18560754, PMCID: PMC2548279, DOI: 10.1007/s00018-008-8195-y.Peer-Reviewed Original ResearchConceptsLong-term administrationPrestin expressionSalicylate administrationChronic salicylate administrationDistortion product otoacoustic emissionsNuclear transcription factors c-fosProduct otoacoustic emissionsTranscription factor c-FosOHC electromotilityHearing lossNF-κBAcute inhibitionOtoacoustic emissionsAdministrationC-fosProtein levelsOuter hair cell electromotilityHair cell electromotilityEgr-1Incremental increaseExpressionSalicylateElectromotilityFour-foldATP activates P2x receptors and requires extracellular Ca++ participation to modify outer hair cell nonlinear capacitance
Yu N, Zhao H. ATP activates P2x receptors and requires extracellular Ca++ participation to modify outer hair cell nonlinear capacitance. Pflügers Archiv - European Journal Of Physiology 2008, 457: 453-461. PMID: 18491132, PMCID: PMC5531446, DOI: 10.1007/s00424-008-0522-5.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAdenosine TriphosphateAnimalsCalciumChelating AgentsEgtazic AcidElectric CapacitanceGuinea PigsHair Cells, Auditory, OuterMembrane PotentialsPatch-Clamp TechniquesPotassiumPurinergic P2 Receptor AntagonistsPyridoxal PhosphateReceptors, Purinergic P2Signal TransductionSodiumSuraminTime FactorsUridine TriphosphateConceptsP2X receptorsATP effectP2Y receptor agonistsMetabotropic purinergic receptorsActive cochlear amplifierEffect of ATPReceptor agonistHearing functionP2Y receptorsPurinergic receptorsDisulfonic acidImportant mediatorReceptorsCell functionOuter hair cell electromotilityHair cell electromotilityIonotropic purinergicCochlear amplifierOHC electromotilityTetraacetic acidATPAgonistsPurinergicVoltage dependenceSuramin
2006
Effects of intense noise exposure on the outer hair cell plasma membrane fluidity
Chen G, Zhao H. Effects of intense noise exposure on the outer hair cell plasma membrane fluidity. Hearing Research 2006, 226: 14-21. PMID: 16870367, DOI: 10.1016/j.heares.2006.06.007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAuditory ThresholdCell MembraneFluorescence Recovery After PhotobleachingGuinea PigsHair Cells, Auditory, OuterHearing Loss, Noise-InducedMembrane FluidityNoisePrestin is expressed on the whole outer hair cell basolateral surface
Yu N, Zhu M, Zhao H. Prestin is expressed on the whole outer hair cell basolateral surface. Brain Research 2006, 1095: 51-58. PMID: 16709400, PMCID: PMC2548272, DOI: 10.1016/j.brainres.2006.04.017.Peer-Reviewed Original Research
2003
Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility
Santos-Sacchi J, Zhao HB. Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility. Pflügers Archiv - European Journal Of Physiology 2003, 446: 617-622. PMID: 12783229, DOI: 10.1007/s00424-003-1053-8.Peer-Reviewed Original Research
2001
Chlorpromazine Alters Outer Hair Cell Electromotility
Lue A, Zhao H, Brownell W. Chlorpromazine Alters Outer Hair Cell Electromotility. Otolaryngology 2001, 125: 71-76. PMID: 11458218, DOI: 10.1067/mhn.2001.116446.Peer-Reviewed Original ResearchConceptsOuter hair cellsHair cellsDose-dependent depolarizationGuinea pig outer hair cellsEffect of chlorpromazineElectromotile responseAntipsychotic medicationChlorpromazine treatmentHearing thresholdsLarge dosesCochlear sensitivityOtoacoustic emissionsInner earChlorpromazineOuter Hair Cell ElectromotilityHair cell electromotilityOHC electromotilityMembrane voltageRecording conditionsCellsMedicationsResponseElectromotility
2000
Directional rectification of gap junctional voltage gating between Dieters cells in the inner ear of guinea pig
Zhao H. Directional rectification of gap junctional voltage gating between Dieters cells in the inner ear of guinea pig. Neuroscience Letters 2000, 296: 105-108. PMID: 11108992, DOI: 10.1016/s0304-3940(00)01626-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCochleaConnexinsDeafnessGap JunctionsGuinea PigsHair Cells, Auditory, OuterLabyrinth Supporting CellsMembrane PotentialsPatch-Clamp TechniquesPotassium ChannelsVoltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane
Oghalai J, Zhao H, Kutz J, Brownell W. Voltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane. Science 2000, 287: 658-661. PMID: 10650000, PMCID: PMC1976274, DOI: 10.1126/science.287.5453.658.Peer-Reviewed Original ResearchConceptsCell plasma membranePlasma membraneMembrane tensionOuter hair cell plasma membraneLipid-protein interactionsMembrane fluidityForce-generating motorsMammalian hearingLipid mobilityLipid lateral diffusionHair cellsOuter hair cellsCell depolarizationExquisite sensitivityMembraneLateral diffusionMedium osmolalityHyposmotic challengeCellsDynamic changesTransmembraneElectromotilityFluidityVoltage dependenceDrugs results
1999
Auditory collusion and a coupled couple of outer hair cells
Zhao H, Santos-Sacchi J. Auditory collusion and a coupled couple of outer hair cells. Nature 1999, 399: 359-362. PMID: 10360573, DOI: 10.1038/20686.Peer-Reviewed Original Research