2021
Small loci of astroglial glutamine synthetase deficiency in the postnatal brain cause epileptic seizures and impaired functional connectivity
Farina MG, Sandhu MRS, Parent M, Sanganahalli BG, Derbin M, Dhaher R, Wang H, Zaveri HP, Zhou Y, Danbolt NC, Hyder F, Eid T. Small loci of astroglial glutamine synthetase deficiency in the postnatal brain cause epileptic seizures and impaired functional connectivity. Epilepsia 2021, 62: 2858-2870. PMID: 34536233, PMCID: PMC9006438, DOI: 10.1111/epi.17072.Peer-Reviewed Original ResearchConceptsPostnatal brainFunctional connectivityContinuous video-electroencephalographic recordingSpontaneous recurrent seizuresPathogenesis of epilepsyGlutamine synthetase deficiencyVideo-electroencephalographic recordingsSpecific animal modelsEx vivo studySeizure thresholdAdult patientsRecurrent seizuresFocal epilepsyAdeno-associated virusHippocampal formationAnimal modelsFocal mannerEpileptic seizuresGS deficiencySmall lociSynthetase deficiencyEpilepsyGradual returnVivo studiesBrain
2018
Astrocytes and Glutamine Synthetase in Epileptogenesis
Eid T, Lee T, Patrylo P, Zaveri HP. Astrocytes and Glutamine Synthetase in Epileptogenesis. Journal Of Neuroscience Research 2018, 97: 1345-1362. PMID: 30022509, PMCID: PMC6338538, DOI: 10.1002/jnr.24267.Peer-Reviewed Original ResearchConceptsMesial temporal lobe epilepsyTemporal lobe epilepsyLobe epilepsyAstrocytic glutamine synthetaseRole of astrocytesPotassium channel Kir4.1Important causative factorExcitatory amino acid transporters EAAT1Antiepileptogenic interventionsEpileptogenic insultAntiepileptogenic therapiesEpilepsy developmentChannel Kir4.1Aquaporin-4EpilepsyCausative factorsTransporters EAAT1New targetsSuch interventionsTransporters MCT1DysfunctionAstrocytesMetabolic mechanismsGlutamine synthetaseDownstream effects
2015
Effects of site-specific infusions of methionine sulfoximine on the temporal progression of seizures in a rat model of mesial temporal lobe epilepsy
Dhaher R, Wang H, Gruenbaum SE, Tu N, Lee TS, Zaveri HP, Eid T. Effects of site-specific infusions of methionine sulfoximine on the temporal progression of seizures in a rat model of mesial temporal lobe epilepsy. Epilepsy Research 2015, 115: 45-54. PMID: 26220375, PMCID: PMC4677790, DOI: 10.1016/j.eplepsyres.2015.05.005.Peer-Reviewed Original ResearchConceptsMesial temporal lobe epilepsyTemporal lobe epilepsyRecurrent seizuresLateral ventricleLobe epilepsyDentate gyrusEntorhinal cortexDeep entorhinal cortexEntorhinal-hippocampal areaWeek recording periodSite-specific infusionPhosphate-buffered salineDays of infusionAdult laboratory ratsRecording periodMethionine sulfoximineSeizure severitySeizure activitySevere seizuresMetabolism of glutamateRat modelSynaptic transmissionAnatomical distributionHippocampal formationAngular bundle
2012
Evidence for astrocytes as a potential source of the glutamate excess in temporal lobe epilepsy
Perez EL, Lauritzen F, Wang Y, Lee TS, Kang D, Zaveri HP, Chaudhry FA, Ottersen OP, Bergersen LH, Eid T. Evidence for astrocytes as a potential source of the glutamate excess in temporal lobe epilepsy. Neurobiology Of Disease 2012, 47: 331-337. PMID: 22659305, PMCID: PMC3392431, DOI: 10.1016/j.nbd.2012.05.010.Peer-Reviewed Original ResearchConceptsTemporal lobe epilepsyRecurrent seizuresConcentration of glutamateHippocampal formationLobe epilepsyExtracellular glutamateRefractory temporal lobe epilepsyEpileptogenic hippocampal formationExtracellular brain glutamateSubset of patientsSaline-treated ratsStart of infusionRatio of glutamateExcessive glutamateGlutamate excessBrain glutamateExtracellular brainAstrocytesExcessive releaseGS deficiencyRatsSeizuresGlutamateSeparate groupsEpilepsy
2011
Altered expression of brain monocarboxylate transporter 1 in models of temporal lobe epilepsy
Lauritzen F, Perez EL, Melillo ER, Roh JM, Zaveri HP, Lee TS, Wang Y, Bergersen LH, Eid T. Altered expression of brain monocarboxylate transporter 1 in models of temporal lobe epilepsy. Neurobiology Of Disease 2011, 45: 165-176. PMID: 21856423, PMCID: PMC3351090, DOI: 10.1016/j.nbd.2011.08.001.Peer-Reviewed Original ResearchConceptsTemporal lobe epilepsyMonocarboxylate transporter 1Hippocampal formationLobe epilepsyAnimal modelsModel of TLEPathogenesis of TLEHuman temporal lobe epilepsyPathophysiology of TLETransporter 1Epileptogenic hippocampal formationNonepileptic control animalsPerforant pathway stimulationRecurrent limbic seizuresRelevant rat modelGlutamine synthetase deficiencyLimbic seizuresAntiepileptic drugsBrain metabolismEpileptogenic brainMicrovessel densityRat modelValproic acidControl animalsMCT1 expression