Featured Publications
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution
Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N, Davidson MW, Gustafsson MG. Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 109: e135-e143. PMID: 22160683, PMCID: PMC3271870, DOI: 10.1073/pnas.1107547108.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyUltralow light intensitiesSuperresolution imaging methodExcited statesFluorophore excited stateStructured illumination microscopyLight intensityRequired nonlinearityResolution extensionHigh light intensityActin cytoskeletonCellular structureReversible photoswitchingNuclear poresNonlinear responseIllumination intensitySuch nonlinear responsesPhotoswitchable proteinsSpatial resolutionFluorescent proteinBiological samplesSix-orderImaging methodMicroscopyPolystyrene beadsSuper-resolution 3D microscopy of live whole cells using structured illumination
Shao L, Kner P, Rego EH, Gustafsson MG. Super-resolution 3D microscopy of live whole cells using structured illumination. Nature Methods 2011, 8: 1044-1046. PMID: 22002026, DOI: 10.1038/nmeth.1734.Peer-Reviewed Original Research
2014
Quantitative analysis and modeling of katanin function in flagellar length control
Kannegaard E, Rego E, Schuck S, Feldman J, Marshall W. Quantitative analysis and modeling of katanin function in flagellar length control. Molecular Biology Of The Cell 2014, 25: 3686-3698. PMID: 25143397, PMCID: PMC4230626, DOI: 10.1091/mbc.e14-06-1116.Peer-Reviewed Original ResearchConceptsFlagellar length controlFlagellar lengthCytoplasmic microtubulesFull-length flagellaHalf-length flagellaLength controlPools of tubulinFlagellar assemblyTranscriptional regulationKatanin functionKatanin-mediated severingOrganelle sizeRegulatory subunitChlamydomonas reinhardtiiCandidate genesMicrotubule severingPreexisting poolFlagellaRegulatory systemPrecursor proteinModel systemGenesSimple model systemMicrotubulesMutations