Featured Publications
Super-resolution 3D microscopy of live whole cells using structured illumination
Shao L, Kner P, Rego EH, Gustafsson MG. Super-resolution 3D microscopy of live whole cells using structured illumination. Nature Methods 2011, 8: 1044-1046. PMID: 22002026, DOI: 10.1038/nmeth.1734.Peer-Reviewed Original Research
2014
Practical Structured Illumination Microscopy
Rego EH, Shao L. Practical Structured Illumination Microscopy. Methods In Molecular Biology 2014, 1251: 175-192. PMID: 25391800, DOI: 10.1007/978-1-4939-2080-8_10.Peer-Reviewed Original Research
2012
Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination
Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 5311-5315. PMID: 22431626, PMCID: PMC3325651, DOI: 10.1073/pnas.1119262109.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyOne-color excitationWide field natureDiffraction-limited resolutionLiquid crystal devicesSpecial fluorophoresCrystal devicesSIM setupExcitation wavelengthAcquisition speedHigh frame rateLight intensityFrame rateBiological structuresLive-cell imagingMultiple colorsMicroscopyHigh light intensityWavelengthLive cellsExcitationResolutionImaging
2011
PALM and STORM: Unlocking live‐cell super‐resolution
Henriques R, Griffiths C, Rego E, Mhlanga M. PALM and STORM: Unlocking live‐cell super‐resolution. Biopolymers 2011, 95: 322-331. PMID: 21254001, DOI: 10.1002/bip.21586.ChaptersMeSH KeywordsBuffersFluorescent DyesLuminescent ProteinsMicroscopyMicroscopy, FluorescenceNanotechnologyPhotochemical ProcessesSingle-Cell AnalysisConceptsLive-cell imaging approachesFluorescence light microscopySuper-resolution microscopySingle-molecule levelCellular biologyLocalization microscopy methodsLight microscopySuper-resolution imagingSingle-molecule localization microscopy methodsBiologyMolecule levelFluorescent markersRecent breakthroughsRecent advancesMicroscopy methodsLight microscopeImaging approachDynamic interactionMolecular visualizationPalmImportant toolCellsMicroscopyImportant goalMarkers