Human trophoblast-extracellular matrix (ECM) interactions in vitro: ECM thickness modulates morphology and proteolytic activity.
Kliman HJ, Feinberg RF. Human trophoblast-extracellular matrix (ECM) interactions in vitro: ECM thickness modulates morphology and proteolytic activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 3057-3061. PMID: 2326266, PMCID: PMC53833, DOI: 10.1073/pnas.87.8.3057.Peer-Reviewed Original ResearchConceptsJEG-3 human choriocarcinoma cellsMatrix degradationExtracellular matrix proteinsProteolytic activityHuman choriocarcinoma cellsExtracellular matrix degradationHuman trophoblastsCellular behaviorVascular developmentMatrix proteinsExtracellular matrix thicknessIntercellular processesMatrix interactionsUterine extracellular matrixExtracellular matrixProteolytic processesCellular morphologyUteroplacental vascular developmentChoriocarcinoma cellsECM thicknessStriking exampleMatrigelMillicell systemProteinMillicell filtersAggregation of dispersed human cytotrophoblastic cells: Lessons relevant to the morphogenesis of the placenta
Babalola G, Coutifaris C, Soto E, Kliman H, Shuman H, Strauss J. Aggregation of dispersed human cytotrophoblastic cells: Lessons relevant to the morphogenesis of the placenta. Developmental Biology 1990, 137: 100-108. PMID: 2295358, DOI: 10.1016/0012-1606(90)90011-7.Peer-Reviewed Original ResearchConceptsJEG-3 cellsSuspension culturesCell-cell associationsJEG-3 choriocarcinoma cellsProtein synthesis inhibitorMulticellular structuresCellular aggregationHigh cell densityTrophoblast cell surfaceCytotrophoblast cellsHuman cytotrophoblastic cellsPlacenta formsCell typesRNA synthesisCell surfacePoor mediumChoriocarcinoma cellsCell linesSerum-free mediumActinomycin DOutgrowth culturesJEG-3Synthesis inhibitorExtent of aggregationFree medium