1990
Human trophoblast-extracellular matrix (ECM) interactions in vitro: ECM thickness modulates morphology and proteolytic activity.
Kliman HJ, Feinberg RF. Human trophoblast-extracellular matrix (ECM) interactions in vitro: ECM thickness modulates morphology and proteolytic activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 3057-3061. PMID: 2326266, PMCID: PMC53833, DOI: 10.1073/pnas.87.8.3057.Peer-Reviewed Original ResearchConceptsJEG-3 human choriocarcinoma cellsMatrix degradationExtracellular matrix proteinsProteolytic activityHuman choriocarcinoma cellsExtracellular matrix degradationHuman trophoblastsCellular behaviorVascular developmentMatrix proteinsExtracellular matrix thicknessIntercellular processesMatrix interactionsUterine extracellular matrixExtracellular matrixProteolytic processesCellular morphologyUteroplacental vascular developmentChoriocarcinoma cellsECM thicknessStriking exampleMatrigelMillicell systemProteinMillicell filters
1988
Biosynthesis and turnover of a 34‐kDa protein growth factor in human cytotrophoblasts
ROY‐CHOUDHURY S, SEN‐MAJUMDAR A, MURTHY U, MISHRA V, KLIMAN H, NESTLER J, STRAUSS J, Manjusri D. Biosynthesis and turnover of a 34‐kDa protein growth factor in human cytotrophoblasts. The FEBS Journal 1988, 172: 777-783. PMID: 3350024, DOI: 10.1111/j.1432-1033.1988.tb13957.x.Peer-Reviewed Original ResearchConceptsProtein growth factorsWestern blot analysisMolecular massPolypeptide molecular massPartial amino acid sequenceAmino acid sequenceBlot analysisGrowth factorMetabolic labeling experimentsPulse-chase experimentsCellular mRNAsVitro translationAcid sequenceReceptor binding specificityBinding specificityIntact cellsLow molecular massBiosynthesisCultured cytotrophoblastsProteinMultinuclear syncytiotrophoblastHuman placentaLabeling experimentsHuman cytotrophoblastsCells
1980
Association of glyceraldehyde-3-phosphate dehydrogenase with the human red cell membrane. A kinetic analysis.
Kliman HJ, Steck TL. Association of glyceraldehyde-3-phosphate dehydrogenase with the human red cell membrane. A kinetic analysis. Journal Of Biological Chemistry 1980, 255: 6314-6321. PMID: 7391020, DOI: 10.1016/s0021-9258(18)43739-8.Peer-Reviewed Original ResearchConceptsGlyceraldehyde-3-phosphate dehydrogenaseBand 3Band 3 proteinHuman red cell membraneRed cell membraneIntact red cellsCell membraneCatalytic siteHuman erythrocyte membranesEnzymeNADH siteRelease of enzymesProteinRate-limiting barrierMembraneAssociation rateNADHErythrocyte membranesSaponin lysis