2010
Role of DAB2IP in modulating epithelial-to-mesenchymal transition and prostate cancer metastasis
Xie D, Gore C, Liu J, Pong RC, Mason R, Hao G, Long M, Kabbani W, Yu L, Zhang H, Chen H, Sun X, Boothman DA, Min W, Hsieh JT. Role of DAB2IP in modulating epithelial-to-mesenchymal transition and prostate cancer metastasis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 2485-2490. PMID: 20080667, PMCID: PMC2823864, DOI: 10.1073/pnas.0908133107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninBlotting, WesternCadherinsCell LineCell Line, TumorCell MovementEpithelial CellsGene ExpressionHumansImmunohistochemistryMaleMesodermMiceMice, NudeNeoplasm MetastasisNeoplasms, ExperimentalProstatic NeoplasmsRas GTPase-Activating ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingTCF Transcription FactorsTransfectionTransplantation, HeterologousVimentinConceptsProstate cancerMesenchymal transitionDAB2IP expressionCarcinoma cellsMultiple lymph nodesMetastatic prostate cancerDistant organ metastasisAggressive prostate cancerMetastatic PCa cellsProstate cancer metastasisClinical prostate cancer specimensHuman normal prostatePotential therapeutic targetXenograft mouse modelProstate cancer specimensProstate carcinoma cellsLymph nodesOrgan metastasisPCa cellsRole of DAB2IPPrognostic biomarkerPCa metastasisKnockout miceTherapeutic targetHuman carcinoma cells
2009
AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling2
Wan T, Liu T, Zhang H, Tang S, Min W. AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling2. Journal Of Biological Chemistry 2009, 285: 3750-3757. PMID: 19948740, PMCID: PMC2823516, DOI: 10.1074/jbc.m109.069385.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingADP-Ribosylation Factor 6ADP-Ribosylation FactorsAmino Acid SequenceAnimalsCarrier ProteinsCattleCell LineCells, CulturedChlorocebus aethiopsCOS CellsGTPase-Activating ProteinsGuanylate KinasesHumansImmunoblottingLipopolysaccharidesMembrane GlycoproteinsMiceMice, KnockoutMitogen-Activated Protein KinasesMolecular Sequence DataMyeloid Differentiation Factor 88NF-kappa BPhosphatidylinositol 4,5-DiphosphateProtein BindingReceptors, Interleukin-1Sequence Homology, Amino AcidToll-Like Receptor 4TransfectionConceptsGTPase-activating proteinsArf6 GAPAIP1 functionsNovel GTPase-activating proteinInhibition of ARF6Pleckstrin homologyGAP domainAdaptor proteinSmall GTPaseDisrupts formationPlasma membraneAIP1MAPK pathwayLipid precursorsToll-like receptor 4Arf6NF-kappaBComplex componentsToll-like receptorsProteinRich sitesGTPaseHomologyComplexesCells increasesDAB2IP coordinates both PI3K-Akt and ASK1 pathways for cell survival and apoptosis
Xie D, Gore C, Zhou J, Pong RC, Zhang H, Yu L, Vessella RL, Min W, Hsieh JT. DAB2IP coordinates both PI3K-Akt and ASK1 pathways for cell survival and apoptosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 19878-19883. PMID: 19903888, PMCID: PMC2785260, DOI: 10.1073/pnas.0908458106.Peer-Reviewed Original ResearchConceptsDAB2IP proteinCell survivalDeath-signaling moleculePI3K-Akt activityPI3K-Akt activationMetastatic prostate cancer cellsPI3K-Akt pathwayCell cycle arrestASK1 activityScaffold proteinPotent growth inhibitorDeath signalsC2 domainSignal moleculesASK1 activationFunctional analysisCell homeostasisApoptotic defectsConstitutive activationJNK pathwayProstate cancer cellsASK1 pathwayPI3K-AktDAB2IP expressionCycle arrestJAK2 and SHP2 Reciprocally Regulate Tyrosine Phosphorylation and Stability of Proapoptotic Protein ASK1*
Yu L, Min W, He Y, Qin L, Zhang H, Bennett AM, Chen H. JAK2 and SHP2 Reciprocally Regulate Tyrosine Phosphorylation and Stability of Proapoptotic Protein ASK1*. Journal Of Biological Chemistry 2009, 284: 13481-13488. PMID: 19287004, PMCID: PMC2679448, DOI: 10.1074/jbc.m809740200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell LineEndothelial CellsEnzyme StabilityHumansInterferon-gammaJanus Kinase 2MAP Kinase Kinase Kinase 5MiceMice, KnockoutMultienzyme ComplexesMutationPhosphorylationProtein Tyrosine Phosphatase, Non-Receptor Type 11Signal TransductionSuppressor of Cytokine Signaling 1 ProteinSuppressor of Cytokine Signaling ProteinsTumor Necrosis Factor-alphaConceptsTyrosine phosphorylationSubstrate-trapping mutantProtein tyrosine phosphatase 2Phosphatase-inactive mutantProteasomal inhibitor MG132ASK1-JNK signalingEndothelial cellsJAK2-specific inhibitorIFN-gamma-induced tyrosine phosphorylationASK1 degradationASK1 dephosphorylationInactive mutantMouse endothelial cellsASK1 phosphorylationPhosphatase 2Inhibitor MG132SHP2Wild typeASK1DephosphorylationMutantsPhosphorylationEnhanced associationJAK2EC apoptosis
2004
AIP1/DAB2IP, a Novel Member of the Ras-GAP Family, Transduces TRAF2-induced ASK1-JNK Activation*
Zhang H, Zhang R, Luo Y, D'Alessio A, Pober JS, Min W. AIP1/DAB2IP, a Novel Member of the Ras-GAP Family, Transduces TRAF2-induced ASK1-JNK Activation*. Journal Of Biological Chemistry 2004, 279: 44955-44965. PMID: 15310755, DOI: 10.1074/jbc.m407617200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCattleCell LineCell MembraneCytoplasmGene DeletionGenes, ReporterGuanylate KinasesHumansImmunoblottingImmunoprecipitationJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4MAP Kinase Kinase Kinase 5Microscopy, ConfocalMicroscopy, FluorescenceMitogen-Activated Protein Kinase KinasesModels, BiologicalMutationNF-kappa BProlineProtein Structure, TertiaryProtein TransportProteinsRas GTPase-Activating ProteinsSignal TransductionTNF Receptor-Associated Factor 2Transfection