2003
COX2 Activity Promotes Organic Osmolyte Accumulation and Adaptation of Renal Medullary Interstitial Cells to Hypertonic Stress*
Moeckel GW, Zhang L, Fogo AB, Hao CM, Pozzi A, Breyer MD. COX2 Activity Promotes Organic Osmolyte Accumulation and Adaptation of Renal Medullary Interstitial Cells to Hypertonic Stress*. Journal Of Biological Chemistry 2003, 278: 19352-19357. PMID: 12637551, DOI: 10.1074/jbc.m302209200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAldehyde ReductaseAnimalsApoptosisBetaineCaspase 3CaspasesCell SurvivalCells, CulturedCyclooxygenase 2Cyclooxygenase 2 InhibitorsCyclooxygenase InhibitorsDehydrationDNA FragmentationGene ExpressionHeat-Shock ProteinsHypertonic SolutionsIn Situ Nick-End LabelingInositolIsoenzymesKidney MedullaMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutProstaglandin-Endoperoxide SynthasesRNA, MessengerSorbitolSymportersTritiumConceptsMedullary interstitial cellsRenal medullary interstitial cellsInterstitial cellsCOX2 activityCOX2 inhibitionReductase mRNA expressionCOX2-specific inhibitorsRenal cell survivalCell survivalAnalgesic nephropathyCOX2 inhibitorsRenal medullaMRNA expressionPmol/Sorbitol accumulationInner medullaOrganic osmolyte accumulationKidney inner medullaMedullaCell deathBetaine concentrationsHypertonic stressPresent studySurvivalInhibitors
2002
Organic osmolytes betaine, sorbitol and inositol are potent inhibitors of erythrocyte membrane ATPases
Moeckel GW, Shadman R, Fogel JM, Sadrzadeh SM. Organic osmolytes betaine, sorbitol and inositol are potent inhibitors of erythrocyte membrane ATPases. Life Sciences 2002, 71: 2413-2424. PMID: 12231402, DOI: 10.1016/s0024-3205(02)02035-0.Peer-Reviewed Original Research
1994
Purification of Human and Rat Kidney Aldose Reductase
Moeckel G, Hallbach J, Guder W. Purification of Human and Rat Kidney Aldose Reductase. Enzyme And Protein 1994, 48: 45-50. PMID: 7787970, DOI: 10.1159/000474968.Peer-Reviewed Original ResearchConceptsPurification of humanAldose reductaseRat kidney papillaRat kidney aldose reductaseSubstrate specificityHuman enzymeAffinity chromatographic procedureBlue SepharoseSDS-PAGESimilar molecular weightReductaseEnzymeMolecular weightSingle bandChromatographic procedureSorbitol formationKinetic constantsKidney papillaHuman kidneyHumansInner medulla