2000
Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50/Rta Protein Activates the Entire Viral Lytic Cycle in the HH-B2 Primary Effusion Lymphoma Cell Line
Gradoville L, Gerlach J, Grogan E, Shedd D, Nikiforow S, Metroka C, Miller G. Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50/Rta Protein Activates the Entire Viral Lytic Cycle in the HH-B2 Primary Effusion Lymphoma Cell Line. Journal Of Virology 2000, 74: 6207-6212. PMID: 10846108, PMCID: PMC112123, DOI: 10.1128/jvi.74.13.6207-6212.2000.Peer-Reviewed Original ResearchDihydrofolate Reductase from Kaposi's Sarcoma-Associated Herpesvirus
Cinquina C, Grogan E, Sun R, Lin S, Beardsley G, Miller G. Dihydrofolate Reductase from Kaposi's Sarcoma-Associated Herpesvirus. Virology 2000, 268: 201-217. PMID: 10683342, DOI: 10.1006/viro.1999.0165.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusDihydrofolate reductaseEarly lytic cycle genesNucleotide metabolism genesDHFR activityHuman DHFRViral enzymesPrimary effusion lymphomaLytic cycle genesKSHV lytic cycleMethionine biosynthesisCycle genesHuman dihydrofolate reductaseCultured B-cell linesMetabolism genesPEL cell linesOwn enzymesB cell linesAmino acidsPEL cellsChemical inductionLytic cycleE. coliInfected cells
1999
Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes
Ragoczy T, Miller G. Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes. Journal Of Virology 1999, 73: 9858-9866. PMID: 10559298, PMCID: PMC113035, DOI: 10.1128/jvi.73.12.9858-9866.1999.Peer-Reviewed Original ResearchConceptsLytic cycle genesRaji cellsEpstein–Barr virus Rta proteinEpstein-Barr virus (EBV) lytic cycleViral targetsLytic cycleVirus lytic cycleLymphoma cell line RajiBurkitt's lymphoma cell line RajiBZLF1 expressionB cell linesCell line RajiBRLF1 geneImmediate early genesInhibitory effectCycle genesViral lytic cycle genesEBVRta proteinViral genesBLRF2CellsExpressionTransactivation functionLate genesKinetics of Kaposi’s Sarcoma-Associated Herpesvirus Gene Expression
Sun R, Lin S, Staskus K, Gradoville L, Grogan E, Haase A, Miller G. Kinetics of Kaposi’s Sarcoma-Associated Herpesvirus Gene Expression. Journal Of Virology 1999, 73: 2232-2242. PMID: 9971806, PMCID: PMC104468, DOI: 10.1128/jvi.73.3.2232-2242.1999.Peer-Reviewed Original ResearchMeSH KeywordsChemokinesGene ExpressionGenes, Immediate-EarlyGenes, ViralHerpesvirus 8, HumanHumansKineticsRNA, ViralSarcoma, KaposiTumor Cells, CulturedConceptsHerpesvirus gene expressionKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusGene expressionLytic cycleEarly genesVIL-6Cell linesKSHV DNA replicationG protein-coupled receptorsProtein-coupled receptorsSingle-cell assaysPrimary effusion lymphoma cell linesImmediate early genesPAN RNADNA replicationEpstein-Barr virus BZLF1Nuclear RNALate genesBovine herpesvirus 4KS biopsiesPEL cell linesVBcl-2Positional homologueKinetic classes
1998
A viral gene that activates lytic cycle expression of Kaposi’s sarcoma-associated herpesvirus
Sun R, Lin S, Gradoville L, Yuan Y, Zhu F, Miller G. A viral gene that activates lytic cycle expression of Kaposi’s sarcoma-associated herpesvirus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10866-10871. PMID: 9724796, PMCID: PMC27987, DOI: 10.1073/pnas.95.18.10866.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCloning, MolecularDNA PrimersDNA, ComplementaryGene Expression Regulation, NeoplasticGenes, ViralHerpesvirus 4, HumanHerpesvirus 8, HumanHumansMolecular Sequence DataSarcoma, KaposiSequence Homology, Amino AcidTranscription, GeneticTumor Cells, CulturedVirus ActivationConceptsEpstein-Barr virusKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusSmall viral capsid antigenPathogenesis of KSHVLytic cycle gene expressionViral capsid antigenLatent viral genomeCapsid antigenLytic cycle genesInterleukin-6Immediate early genesViral cytokineEarly lytic genesLytic genesHerpesvirusVirusViral genesViral genomeExpressionGene expressionCytokinesPathogenesisCycle genesGenes
1990
Inhibition of specific binding of EBNA 1 to DNA by Murine Monoclonal and certain human polyclonal antibodies
Orlowski R, Polvino-Bodnar M, Hearing J, Miller G. Inhibition of specific binding of EBNA 1 to DNA by Murine Monoclonal and certain human polyclonal antibodies. Virology 1990, 176: 638-642. PMID: 2161154, DOI: 10.1016/0042-6822(90)90036-q.Peer-Reviewed Original ResearchConceptsEBNA-1 proteinGel mobility shift experimentsMobility shift experimentsSequence-specific DNARecognition of DNAEBNA-1Binding of DNASpecific bindingLac promoterDNA bindingMonoclonal antibodiesDimerization siteSpecific DNANonfusion proteinBurkitt's lymphoma cellsShift experimentsMolecular massEscherichia coliProteinDNASame monoclonal antibodyBindingPolyclonal antibodiesEB virusLymphoma cells