2008
Structural Basis for Toxin Resistance of β4-Associated Calcium-activated Potassium (BK) Channels*
Gan G, Yi H, Chen M, Sun L, Li W, Wu Y, Ding J. Structural Basis for Toxin Resistance of β4-Associated Calcium-activated Potassium (BK) Channels*. Journal Of Biological Chemistry 2008, 283: 24177-24184. PMID: 18559348, PMCID: PMC3259794, DOI: 10.1074/jbc.m800179200.Peer-Reviewed Original ResearchConceptsAlpha subunitBeta subunitStructural basisWide open conformationAuxiliary beta subunitsFunctional diversityInternal binding siteToxin resistanceBasic residuesOpen conformationChannel poreSubunitsDiverse interactionsBinding sitesLarge conductancePotassium channelsMSloChannel inhibitorsMutantsDiversityResiduesSitesBetter understandingInteraction
2007
Four-turn α-Helical Segment Prevents Surface Expression of the Auxiliary hβ2 Subunit of BK-type Channel*
Lv C, Chen M, Gan G, Wang L, Xu T, Ding J. Four-turn α-Helical Segment Prevents Surface Expression of the Auxiliary hβ2 Subunit of BK-type Channel*. Journal Of Biological Chemistry 2007, 283: 2709-2715. PMID: 17991741, DOI: 10.1074/jbc.m704440200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineEndoplasmic ReticulumGene ExpressionHumansLarge-Conductance Calcium-Activated Potassium ChannelsMicroscopy, FluorescenceModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedPatch-Clamp TechniquesProtein Structure, SecondaryProtein SubunitsRatsRecombinant Fusion ProteinsSequence Homology, Amino AcidConceptsBK-type channelsSurface expressionRat chromaffin cellsExtracellular loopPancreatic beta cellsTrafficking mechanismsN-terminusDRG neuronsHelical segmentsToxin sensitivityBeta cellsClamp techniqueHelp of immunofluorescenceAuxiliary beta subunitsAlpha-helical segmentsChromaffin cellsBeta2 subunitBK channelsHbeta2Retention signalChannel regulatorRegulatory mechanismsBeta subunitEndoplasmic reticulumLarge conductanceMolecular cloning and electrophysiological studies on the first K+ channel toxin (LmKTx8) derived from scorpion Lychas mucronatus
Wu W, Yin S, Ma Y, Wu Y, Zhao R, Gan G, Ding J, Cao Z, Li W. Molecular cloning and electrophysiological studies on the first K+ channel toxin (LmKTx8) derived from scorpion Lychas mucronatus. Peptides 2007, 28: 2306-2312. PMID: 18006119, DOI: 10.1016/j.peptides.2007.10.009.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCell LineCloning, MolecularDNA PrimersDNA, ComplementaryElectrophysiologyHumansKv1.3 Potassium ChannelModels, MolecularMolecular Sequence DataMolecular WeightPatch-Clamp TechniquesPotassium Channel BlockersRecombinant Fusion ProteinsScorpion VenomsScorpionsSequence Homology, Amino AcidSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization