2023
Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransporters
2016
FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments
Pincet F, Adrien V, Yang R, Delacotte J, Rothman JE, Urbach W, Tareste D. FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments. PLOS ONE 2016, 11: e0158457. PMID: 27387979, PMCID: PMC4936743, DOI: 10.1371/journal.pone.0158457.Peer-Reviewed Original ResearchConceptsFluorescence correlation spectroscopySingle-particle trackingCorresponding recovery timeFRAP measurementsDynamics of lipidsDiffusion coefficientCorrelation spectroscopyBrownian motionParticle trackingConfocal microscopeAccurate valuesDiffusive speciesMembrane environmentMolecular diffusionMeasurementsMembrane platformsBehavior of lipidsFRAP experimentsMotion
2007
Hydrophobic Forces and Hydrogen Bonds in the Adhesion between Retinoid-Coated Surfaces
Tareste D, Pincet F, Lebeau L, Perez É. Hydrophobic Forces and Hydrogen Bonds in the Adhesion between Retinoid-Coated Surfaces. Langmuir 2007, 23: 3225-3229. PMID: 17266339, DOI: 10.1021/la0629779.Peer-Reviewed Original ResearchConceptsHydrophobic forcesHydrogen bondsPure waterShort-range attractive forcesLipid layerHydrophilic headgroupLong-range attractionHydrophobic chainsTris bufferHydrophobic effectLipid monolayersApolar characterMolecular interactionsAttractive forceBondsLipid bilayersExponential forceHeadgroupsNew classForce measurementsDistance profilesWaterBufferInteractionMonolayers
2005
Giant vesicles formed by gentle hydration and electroformation: A comparison by fluorescence microscopy
Rodriguez N, Pincet F, Cribier S. Giant vesicles formed by gentle hydration and electroformation: A comparison by fluorescence microscopy. Colloids And Surfaces B Biointerfaces 2005, 42: 125-130. PMID: 15833663, DOI: 10.1016/j.colsurfb.2005.01.010.Peer-Reviewed Original ResearchThe Natural LewisX‐Bearing Lipids Promote Membrane Adhesion: Influence of Ceramide on Carbohydrate–Carbohydrate Recognition
Gourier C, Pincet F, Perez E, Zhang Y, Zhu Z, Mallet J, Sinaÿ P. The Natural LewisX‐Bearing Lipids Promote Membrane Adhesion: Influence of Ceramide on Carbohydrate–Carbohydrate Recognition. Angewandte Chemie International Edition 2005, 44: 1683-1687. PMID: 15693050, DOI: 10.1002/anie.200461224.Peer-Reviewed Original Research
2001
From Macroscopic Adhesion Energy to Molecular Bonds: A Test of the Theory
Pincet F, Perez E, Loudet J, Lebeau L. From Macroscopic Adhesion Energy to Molecular Bonds: A Test of the Theory. Physical Review Letters 2001, 87: 178101. PMID: 11690314, DOI: 10.1103/physrevlett.87.178101.Peer-Reviewed Original Research