2018
Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+
Gruget C, Coleman J, Bello O, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH. Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+. FEBS Letters 2018, 592: 1497-1506. PMID: 29578584, DOI: 10.1002/1873-3468.13040.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCell MembraneDose-Response Relationship, DrugMagnesiumProtein BindingSurface PropertiesSynaptotagmin IThermodynamics
2007
Mapping Mouse Gamete Interaction Forces Reveal Several Oocyte Membrane Regions with Different Mechanical and Adhesive Properties †
Jégou A, Pincet F, Perez E, Wolf JP, Ziyyat A, Gourier C. Mapping Mouse Gamete Interaction Forces Reveal Several Oocyte Membrane Regions with Different Mechanical and Adhesive Properties †. Langmuir 2007, 24: 1451-1458. PMID: 18027975, DOI: 10.1021/la702258x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell MembraneFemaleMaleMiceMice, Inbred CBAOocytesSperm-Ovum InteractionsSpermatocytesStress, MechanicalSurface PropertiesConfinement Free Energy of Surfaces Bearing End-Grafted Polymers in the Mushroom Regime and Local Measurement of the Polymer Density
Li F, Pincet F. Confinement Free Energy of Surfaces Bearing End-Grafted Polymers in the Mushroom Regime and Local Measurement of the Polymer Density. Langmuir 2007, 23: 12541-12548. PMID: 17988162, DOI: 10.1021/la7021374.Peer-Reviewed Original ResearchConceptsPolymer chainsMushroom regimeFree energyEnd-Grafted PolymersSurface force measurementsSingle polymer chainSurface force apparatusEnd-tethered polymer chainsInteraction free energyGrafted polymersForce apparatusPolymer densityForce profilesPolymersConfinement free energyPolymer theoryLipid bilayersIdentical surfacesMushroomlike structuresFree energy approximationForce measurementsSurfacePlanar surfaceGood precisionChainEnergetics and dynamics of SNAREpin folding across lipid bilayers
Li F, Pincet F, Perez E, Eng WS, Melia TJ, Rothman JE, Tareste D. Energetics and dynamics of SNAREpin folding across lipid bilayers. Nature Structural & Molecular Biology 2007, 14: 890-896. PMID: 17906638, DOI: 10.1038/nsmb1310.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsLipid BilayersMembrane FusionMiceProtein ConformationProtein FoldingRatsSNARE ProteinsSurface PropertiesHydrophobic Forces and Hydrogen Bonds in the Adhesion between Retinoid-Coated Surfaces
Tareste D, Pincet F, Lebeau L, Perez É. Hydrophobic Forces and Hydrogen Bonds in the Adhesion between Retinoid-Coated Surfaces. Langmuir 2007, 23: 3225-3229. PMID: 17266339, DOI: 10.1021/la0629779.Peer-Reviewed Original ResearchConceptsHydrophobic forcesHydrogen bondsPure waterShort-range attractive forcesLipid layerHydrophilic headgroupLong-range attractionHydrophobic chainsTris bufferHydrophobic effectLipid monolayersApolar characterMolecular interactionsAttractive forceBondsLipid bilayersExponential forceHeadgroupsNew classForce measurementsDistance profilesWaterBufferInteractionMonolayers
2004
Specific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics
Sarda S, Pointu D, Pincet F, Henry N. Specific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics. Biophysical Journal 2004, 86: 3291-3303. PMID: 15111442, PMCID: PMC1304194, DOI: 10.1016/s0006-3495(04)74377-5.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD19Binding SitesBiophysicsBiotinylationCell AdhesionCell Line, TransformedCell Line, TumorCell MembraneDiffusionFlow CytometryHumansKineticsLigandsLymphoma, B-CellMicroscopy, FluorescenceModels, BiologicalProtein BindingScattering, RadiationStreptavidinStress, MechanicalSurface PropertiesTemperatureTime Factors
2002
Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid Layers
Tareste D, Pincet F, Perez E, Rickling S, Mioskowski C, Lebeau L. Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid Layers. Biophysical Journal 2002, 83: 3675-3681. PMID: 12496134, PMCID: PMC1302442, DOI: 10.1016/s0006-3495(02)75367-8.Peer-Reviewed Original ResearchConceptsHydrogen bondsIntramolecular hydrogen bondsHydrogen-bonding groupsSurface force apparatusSingle hydrogen bondHydrogen-bonding partsMonolayer of lipidsDifferent energetic contributionsForce apparatusHydrophobic interactionsInteraction energyBondsKcal/Lipid layerEnergetic contributionsTheoretical studyAdhesion energyProtein systemsBond partProtein foldingProtein stabilizationHeadgroupsWaterSurfaceComplex structure
2001
From Macroscopic Adhesion Energy to Molecular Bonds: A Test of the Theory
Pincet F, Perez E, Loudet J, Lebeau L. From Macroscopic Adhesion Energy to Molecular Bonds: A Test of the Theory. Physical Review Letters 2001, 87: 178101. PMID: 11690314, DOI: 10.1103/physrevlett.87.178101.Peer-Reviewed Original Research
1994
Do Trehalose and Dimethyl Sulfoxide Affect Intermembrane Forces?
Pincet F, Perez E, Wolfe J. Do Trehalose and Dimethyl Sulfoxide Affect Intermembrane Forces? Cryobiology 1994, 31: 531-539. PMID: 7835052, DOI: 10.1006/cryo.1994.1064.Peer-Reviewed Original Research