1997
Distribution of a Synthetic Protease Inhibitor in Rat Pancreatic Acini After Supramaximal Secretagogue Stimulation
Otani T, Atomi Y, Kuroda A, Muto T, Tamura M, Fukuda S, Akao S, Gorelick F. Distribution of a Synthetic Protease Inhibitor in Rat Pancreatic Acini After Supramaximal Secretagogue Stimulation. Pancreas 1997, 14: 142-149. PMID: 9057186, DOI: 10.1097/00006676-199703000-00006.Peer-Reviewed Original ResearchConceptsAcinar cellsPancreatic aciniProtease inhibitorsLow molecular weight protease inhibitorSynthetic protease inhibitorLarge cytoplasmic vacuolesIsolated pancreatic aciniRat pancreatic aciniAcute pancreatitisWeight protease inhibitorsZymogen granulesCerulein treatmentE3123Supramaximal secretagogue stimulationVivo modelElectron microscopic autoradiographyBeneficial effectsMin of incubationUnstimulated controlsMicroscopic autoradiographyInhibition of proteasesCytoplasmic vacuolesAciniPancreatitisFormation of vacuoles
1991
Intracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin.
Leach SD, Modlin IM, Scheele GA, Gorelick FS. Intracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin. Journal Of Clinical Investigation 1991, 87: 362-366. PMID: 1985109, PMCID: PMC295064, DOI: 10.1172/jci114995.Peer-Reviewed Original ResearchConceptsPancreatic aciniIntracellular activationCysteine protease inhibitor E64Digestive zymogensDose of cholecystokininRat pancreatic aciniProtease inhibitor E64Acute pancreatitisCholecystokininHigh dosesSerine protease activityAcidic subcellular compartmentsWeak base chloroquineImmunologic techniquesInhibitor E64Protonophore monensinPancreatitisIncubation mediumAciniBase chloroquineActivationIntracellular sitesProtease inhibitor benzamidineCell pelletsElectrophoretic mobility