2004
Vacuolar ATPase Regulates Zymogen Activation in Pancreatic Acini*
Waterford SD, Kolodecik TR, Thrower EC, Gorelick FS. Vacuolar ATPase Regulates Zymogen Activation in Pancreatic Acini*. Journal Of Biological Chemistry 2004, 280: 5430-5434. PMID: 15582989, PMCID: PMC2846595, DOI: 10.1074/jbc.m413513200.Peer-Reviewed Original ResearchMeSH KeywordsAmylasesAnimalsCalciumCarbacholCell MembraneCells, CulturedCeruletideChloroquineChymotrypsinEnzyme ActivationEnzyme PrecursorsHydrogen-Ion ConcentrationMacrolidesMaleMonensinPancreasProtein SubunitsProtein TransportRatsRats, Sprague-DawleySolubilityThapsigarginTrypsinVacuolar Proton-Translocating ATPasesConceptsPancreatic acinar cellsSupramaximal concentrationsPancreatic aciniAcinar cellsVacuolar ATPase inhibitor bafilomycinConcentration-dependent mannerAcute pancreatitisEffects of agentsATPase inhibitor bafilomycinConcentration-dependent translocationWeak base chloroquineCaerulein stimulationIntracellular pHConcanamycin AChymotrypsin activationActivationBase chloroquineV-ATPase activationInhibitor bafilomycin
1991
Intracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin.
Leach SD, Modlin IM, Scheele GA, Gorelick FS. Intracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin. Journal Of Clinical Investigation 1991, 87: 362-366. PMID: 1985109, PMCID: PMC295064, DOI: 10.1172/jci114995.Peer-Reviewed Original ResearchConceptsPancreatic aciniIntracellular activationCysteine protease inhibitor E64Digestive zymogensDose of cholecystokininRat pancreatic aciniProtease inhibitor E64Acute pancreatitisCholecystokininHigh dosesSerine protease activityAcidic subcellular compartmentsWeak base chloroquineImmunologic techniquesInhibitor E64Protonophore monensinPancreatitisIncubation mediumAciniBase chloroquineActivationIntracellular sitesProtease inhibitor benzamidineCell pelletsElectrophoretic mobility