2022
A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6
Schmidt JM, Yang R, Kumar A, Hunker O, Seebacher J, Bleichert F. A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6. Nature Communications 2022, 13: 1059. PMID: 35217664, PMCID: PMC8881611, DOI: 10.1038/s41467-022-28695-w.Peer-Reviewed Original ResearchConceptsOrigin recognition complexS. cerevisiaeCyclin-dependent kinase phosphorylationMcm2-7 loadingN-terminal domainCryo-electron microscopyCDK phosphorylationRecognition complexDNA replicationReplication originsÅ resolutionKinase phosphorylationMechanism of originCdc6Coordinated actionCerevisiaePhosphorylationDNAInhibitory signalsStructural detailsSite regulationRecruitmentOrc6AssemblyCdt1
2019
Mechanisms of replication origin licensing: a structural perspective
Bleichert F. Mechanisms of replication origin licensing: a structural perspective. Current Opinion In Structural Biology 2019, 59: 195-204. PMID: 31630057, DOI: 10.1016/j.sbi.2019.08.007.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsReplicative helicasesKey cell cycle eventsReplication origin licensingReplication start sitesCell cycle eventsOrigin licensingReplicative helicaseReplication initiationInitiation factorsStart siteChromosomal DNAReplicative machineryCycle eventsMolecular approachesSynthesis apparatusHelicasesMachineryStructural perspectiveBidirectional mannerDNAEukaryotesHelicaseRecent advancesDuplicationMechanism
2017
Mechanisms for initiating cellular DNA replication
Bleichert F, Botchan MR, Berger JM. Mechanisms for initiating cellular DNA replication. Science 2017, 355 PMID: 28209641, DOI: 10.1126/science.aah6317.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsArchaeaBacteriaCellsDNA HelicasesDNA ReplicationEukaryotaHelix-Turn-Helix MotifsPhylogenyProtein DomainsConceptsReplicative helicasesDNA replicationRing-shaped hexameric helicasesDNA replication factoriesDNA replication initiationDomains of lifeCellular DNA replicationDNA helixReplisome assemblyReplication initiationMolecular choreographyHexameric helicasesReplication factoriesAccessory proteinsHelicasesDNA synthesisMultistep processDNAHelixReplicationProteinMechanismInitiationAssemblyLoader