2016
Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells
Tiwari M, Adaku N, Peart N, Rogers FA. Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells. Nucleic Acids Research 2016, 44: 7742-7754. PMID: 27298253, PMCID: PMC5027492, DOI: 10.1093/nar/gkw515.Peer-Reviewed Original ResearchConceptsDouble-strand breaksReplication fork collapseFork collapseGenomic instabilityReplication-associated double-strand breaksDNA replication fork progressionNucleotide excision repair proteinsTriplex structureRobust DNA damage responseDNA double-strand breaksReplication fork progressionReplication fork stabilityStrand breaksReplication fork integrityXPC-deficient cellsDNA damage responseMirror repeat sequencesExcision repair proteinsMore DSBsNER-deficient cellsDamage recognition proteinsGenomic integrityDual phosphorylationFork stabilityFork integrity
2009
Targeted correction of a thalassemia-associated β-globin mutation induced by pseudo-complementary peptide nucleic acids
Lonkar P, Kim KH, Kuan JY, Chin JY, Rogers FA, Knauert MP, Kole R, Nielsen PE, Glazer PM. Targeted correction of a thalassemia-associated β-globin mutation induced by pseudo-complementary peptide nucleic acids. Nucleic Acids Research 2009, 37: 3635-3644. PMID: 19364810, PMCID: PMC2699504, DOI: 10.1093/nar/gkp217.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GlobinsCell LineCHO CellsCricetinaeCricetulusGene TargetingHumansMutationPeptide Nucleic AcidsPlasmidsRecombination, GeneticS PhaseThalassemiaXeroderma Pigmentosum Group A ProteinConceptsBeta-globin geneNucleotide excision repair factorsAltered helical structureExcision repair factorsSingle base-pair modificationTriplex-forming peptide nucleic acidsPseudo-complementary peptide nucleic acidsDisease-related genesDonor DNA fragmentsComplementary DNA sequenceNucleic acidsProper splicingRepair factorsSite-specific bindingMammalian cellsSite-specific modificationDNA repairDNA sequencesGene targetingSecond intronDNA fragmentsHuman cellsTriple helix formationGene correctionHuman fibroblast cells
2002
Site-directed recombination via bifunctional PNA–DNA conjugates
Rogers FA, Vasquez KM, Egholm M, Glazer PM. Site-directed recombination via bifunctional PNA–DNA conjugates. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 16695-16700. PMID: 12461167, PMCID: PMC139206, DOI: 10.1073/pnas.262556899.Peer-Reviewed Original ResearchMeSH KeywordsDNADNA RepairDNA-Binding ProteinsHeLa CellsHumansPeptide Nucleic AcidsRecombination, GeneticXeroderma Pigmentosum Group A ProteinConceptsSite-directed recombinationDNA repairNucleotide excision repair factorsExcision repair factorsSite-specific DNASite-specific recombinationHuman cell-free extractsDonor DNA fragmentsRepair factorsMammalian cellsGenetic manipulationCell-free extractsTarget genesSite-directed mannerReporter geneStrand invasionPlasmid substratesDNA fragmentsDefective geneDonor DNAWatson-Crick base pairingDuplex DNAGenesTarget plasmidDNA