Reconstructed Single-Cell Fate Trajectories Define Lineage Plasticity Windows during Differentiation of Human PSC-Derived Distal Lung Progenitors
Hurley K, Ding J, Villacorta-Martin C, Herriges MJ, Jacob A, Vedaie M, Alysandratos KD, Sun YL, Lin C, Werder RB, Huang J, Wilson AA, Mithal A, Mostoslavsky G, Oglesby I, Caballero IS, Guttentag SH, Ahangari F, Kaminski N, Rodriguez-Fraticelli A, Camargo F, Bar-Joseph Z, Kotton DN. Reconstructed Single-Cell Fate Trajectories Define Lineage Plasticity Windows during Differentiation of Human PSC-Derived Distal Lung Progenitors. Cell Stem Cell 2020, 26: 593-608.e8. PMID: 32004478, PMCID: PMC7469703, DOI: 10.1016/j.stem.2019.12.009.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsHuman pluripotent stem cellsLung progenitorsSingle-cell RNACell fate trajectoriesDistal lung progenitorsEndodermal fateLentiviral barcodingSelf-renewal capacityDevelopmental plasticityWnt responseNKX2-1Stem cellsStable phenotypeAlveolar epithelial type 2 cellsProgenitorsFacultative progenitorsPlasticity windowContinuous state Hidden Markov ModelsEpithelial type 2 cellsDifferentiationFateAEC2sType 2 cellsBarcoding