2013
Advancing functional engineered cardiac tissues toward a preclinical model of human myocardium
Turnbull IC, Karakikes I, Serrao GW, Backeris P, Lee J, Xie C, Senyei G, Gordon RE, Li RA, Akar FG, Hajjar RJ, Hulot J, Costa KD. Advancing functional engineered cardiac tissues toward a preclinical model of human myocardium. The FASEB Journal 2013, 28: 644-654. PMID: 24174427, PMCID: PMC3898643, DOI: 10.1096/fj.13-228007.Peer-Reviewed Original ResearchConceptsHuman myocardiumPreclinical modelsCardiac tissueAlternative preclinical modelsAction potential durationFrank-Starling mechanismCycle length dependenceHuman embryonic stem cell-derived cardiomyocytesDose-response curveEmbryonic stem cell-derived cardiomyocytesStem cell-derived cardiomyocytesHuman heart musclePositive chronotropicInotropic responseCardiac refractorinessCell-derived cardiomyocytesTrabecular musclesPotential durationPharmacodynamic modelMRNA expressionMyocardiumHeart muscleCardiac-specific genesTranslational researchCardiac electrophysiology
2008
Effects of 4′-chlorodiazepam on cellular excitation–contraction coupling and ischaemia–reperfusion injury in rabbit heart
Brown DA, Aon MA, Akar FG, Liu T, Sorarrain N, O’Rourke B. Effects of 4′-chlorodiazepam on cellular excitation–contraction coupling and ischaemia–reperfusion injury in rabbit heart. Cardiovascular Research 2008, 79: 141-149. PMID: 18304929, PMCID: PMC2562874, DOI: 10.1093/cvr/cvn053.Peer-Reviewed Original ResearchConceptsIschaemia-reperfusion injuryExcitation-contraction couplingReperfusion arrhythmiasRabbit heartsDose-dependent negative inotropic responseCellular excitation-contraction couplingPost-ischemic cardiac dysfunctionOnset of reperfusionMin of reperfusionSingle bolus doseNegative inotropic responseIschaemia/reperfusionIntracellular calcium transientsSarcolemmal ion channelsIsolated rabbit cardiomyocytesIon channelsCardiac action potentialContractile impairmentCardiac dysfunctionBolus doseContractile dysfunctionInotropic responseGlobal ischaemiaVoltage clamp methodCalcium current