Featured Publications
Differential effects of anti-CD20 therapy on CD4 and CD8 T cells and implication of CD20-expressing CD8 T cells in MS disease activity
Shinoda K, Li R, Rezk A, Mexhitaj I, Patterson K, Kakara M, Zuroff L, Bennett J, von Büdingen H, Carruthers R, Edwards K, Fallis R, Giacomini P, Greenberg B, Hafler D, Ionete C, Kaunzner U, Lock C, Longbrake E, Pardo G, Piehl F, Weber M, Ziemssen T, Jacobs D, Gelfand J, Cross A, Cameron B, Musch B, Winger R, Jia X, Harp C, Herman A, Bar-Or A. Differential effects of anti-CD20 therapy on CD4 and CD8 T cells and implication of CD20-expressing CD8 T cells in MS disease activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2207291120. PMID: 36634138, PMCID: PMC9934304, DOI: 10.1073/pnas.2207291120.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD20CD8-Positive T-LymphocytesFlow CytometryHumansLeukocytes, MononuclearMultiple SclerosisRecurrenceConceptsEarly disease activityDisease activityCD8 T cellsT cellsCD20 therapyPeripheral blood mononuclear cellsCellular immune profilesNew disease activityMS disease activityT cell poolMultiple sclerosis patientsAnti-inflammatory profileBlood mononuclear cellsTreatment-associated changesMultiparametric flow cytometryCentral nervous systemFurther dosingRepeat infusionsImmune profileMS patientsSclerosis patientsValidation cohortMononuclear cellsRelapse developmentImmune cascade
2015
Dimethyl fumarate selectively reduces memory T cells in multiple sclerosis patients
Longbrake E, Ramsbottom M, Cantoni C, Ghezzi L, Cross A, Piccio L. Dimethyl fumarate selectively reduces memory T cells in multiple sclerosis patients. Multiple Sclerosis Journal 2015, 22: 1061-1070. PMID: 26459150, PMCID: PMC4829494, DOI: 10.1177/1352458515608961.Peer-Reviewed Original ResearchConceptsDMF-treated patientsMemory T cellsMultiple sclerosis patientsT cellsMS patientsDendritic cellsSclerosis patientsEffector memory T cellsUntreated MS patientsMyeloid dendritic cellsAbsolute lymphocyte countPlasmacytoid dendritic cellsNatural killer cellsNaïve T cellsMechanism of actionLymphocyte countLymphopenic patientsRegulatory cellsKiller cellsPeripheral bloodImmune cellsHealthy controlsPatientsB cellsFlow cytometry
2011
Deficient CX3CR1 Signaling Promotes Recovery after Mouse Spinal Cord Injury by Limiting the Recruitment and Activation of Ly6Clo/iNOS+ Macrophages
Donnelly DJ, Longbrake EE, Shawler TM, Kigerl KA, Lai W, Tovar CA, Ransohoff RM, Popovich PG. Deficient CX3CR1 Signaling Promotes Recovery after Mouse Spinal Cord Injury by Limiting the Recruitment and Activation of Ly6Clo/iNOS+ Macrophages. Journal Of Neuroscience 2011, 31: 9910-9922. PMID: 21734283, PMCID: PMC3139517, DOI: 10.1523/jneurosci.2114-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAntigens, LyCD11 AntigensCells, CulturedChemokine CXCL1CX3C Chemokine Receptor 1Disease Models, AnimalFlow CytometryGene Expression RegulationGreen Fluorescent ProteinsMacrophagesMiceMice, Inbred C57BLMice, TransgenicMotor ActivityMyelin Basic ProteinNitric OxideNitric Oxide Synthase Type IIReceptors, ChemokineRecovery of FunctionSignal TransductionSpinal Cord InjuriesConceptsSpinal cord injuryMonocyte-derived macrophagesCord injuryMouse spinal cord injuryTraumatic spinal cord injuryBlockade of CX3CR1Novel monocyte subsetsCX3CR1-deficient miceAnti-inflammatory therapySelective anti-inflammatory therapyWild-type miceDistinct macrophage subsetsInflammatory cytokinesMonocyte subsetsChemokine receptorsFunctional improvementSpinal cordInflammatory signalingNeurotoxic effectsPromotes recoveryMacrophage subsetsLesion siteCX3CR1Oxidative metabolitesMice
2007
Characterization and modeling of monocyte‐derived macrophages after spinal cord injury
Longbrake EE, Lai W, Ankeny DP, Popovich PG. Characterization and modeling of monocyte‐derived macrophages after spinal cord injury. Journal Of Neurochemistry 2007, 102: 1083-1094. PMID: 17663750, DOI: 10.1111/j.1471-4159.2007.04617.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB7-1 AntigenCD11b AntigenCytokinesDisease Models, AnimalFemaleFlow CytometryGene Expression RegulationHistocompatibility Antigens Class IIIn Vitro TechniquesMacrophagesMiceMice, Inbred C57BLPhagocytosisReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSpinal Cord InjuriesTime FactorsConceptsSpinal cord injuryMonocyte-derived macrophagesBone marrow-derived macrophagesCord injurySpinal cordContext of SCIMarrow-derived macrophagesNeuroinflammatory reactionThioglycollate-elicited peritonealEffector cellsPeripheral macrophagesResident peritonealCytokine expressionLaser capture microdissectionReal-time PCRMacrophage subsetsMDM differentiationInjuryMacrophagesCapture microdissectionCordPeritonealMDM responseFunctional consequencesFuture studies