2013
Quantitative full time course analysis of nonlinear enzyme cycling kinetics
Cao W, De La Cruz EM. Quantitative full time course analysis of nonlinear enzyme cycling kinetics. Scientific Reports 2013, 3: 2658. PMID: 24029878, PMCID: PMC3772379, DOI: 10.1038/srep02658.Peer-Reviewed Original ResearchConceptsKinetic time coursesProduct inhibitionSteady-state enzyme kinetic parametersReaction schemeEnzymology approachReaction productsReversible bindingEnzyme kinetic parametersProduct releasePractical general methodKinetic parametersEnzyme inhibitionGeneral methodProductsEnzyme systemEnzymatic parametersTake advantage of time in your experiments: a guide to simple, informative kinetics assays
Pollard TD, De La Cruz EM. Take advantage of time in your experiments: a guide to simple, informative kinetics assays. Molecular Biology Of The Cell 2013, 24: 1103-1110. PMID: 23580192, PMCID: PMC3623632, DOI: 10.1091/mbc.e13-01-0030.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsComputer SimulationCytological TechniquesKineticsModels, BiologicalResearch Design
2010
Actin filament remodeling by actin depolymerization factor/cofilin
Pfaendtner J, De La Cruz EM, Voth GA. Actin filament remodeling by actin depolymerization factor/cofilin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 7299-7304. PMID: 20368459, PMCID: PMC2867716, DOI: 10.1073/pnas.0911675107.Peer-Reviewed Original ResearchConceptsActin depolymerization factorSubunit interactionsCofilin bindingActin filamentsADF/cofilinFactor/cofilinLong-pitch helixHelix monomersNeighboring subunitMolecular basisSevering proteinHydrophobic loopConformational dynamicsSubdomain 1Subdomain 2Loop movesTriggers reorganizationCofilinFilament flexibilityFilamentsNative filamentsDNaseFilament contactsAtom simulationsBinding
2009
Chapter 6 Kinetic and Equilibrium Analysis of the Myosin ATPase
De La Cruz EM, Ostap EM. Chapter 6 Kinetic and Equilibrium Analysis of the Myosin ATPase. Methods In Enzymology 2009, 455: 157-192. PMID: 19289206, PMCID: PMC2921708, DOI: 10.1016/s0076-6879(08)04206-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsComputer SimulationDactinomycinEnzyme StabilityHumansKineticsMyosinsProtein BindingThermodynamics
2008
Cofilin Increases the Bending Flexibility of Actin Filaments: Implications for Severing and Cell Mechanics
McCullough BR, Blanchoin L, Martiel JL, De La Cruz EM. Cofilin Increases the Bending Flexibility of Actin Filaments: Implications for Severing and Cell Mechanics. Journal Of Molecular Biology 2008, 381: 550-558. PMID: 18617188, PMCID: PMC2753234, DOI: 10.1016/j.jmb.2008.05.055.Peer-Reviewed Original Research
2000
ADP Inhibition of Myosin V ATPase Activity
De La Cruz E, Sweeney H, Ostap E. ADP Inhibition of Myosin V ATPase Activity. Biophysical Journal 2000, 79: 1524-1529. PMID: 10969013, PMCID: PMC1301045, DOI: 10.1016/s0006-3495(00)76403-4.Peer-Reviewed Original Research