2014
Uncovering Buffered Pleiotropy: A Genome-Scale Screen for mel-28 Genetic Interactors in Caenorhabditis elegans
Fernandez A, Mis E, Lai A, Mauro M, Quental A, Bock C, Piano F. Uncovering Buffered Pleiotropy: A Genome-Scale Screen for mel-28 Genetic Interactors in Caenorhabditis elegans. G3: Genes, Genomes, Genetics 2014, 4: 185-196. PMID: 24281427, PMCID: PMC3887534, DOI: 10.1534/g3.113.008532.Peer-Reviewed Original ResearchConceptsMEL-28Caenorhabditis elegansLethal genesGenetic interaction screensGenome-scale screeningWild-type wormsNuclear envelope functionsCell-matrix attachmentWild-type larvaeDynactin functionGenetic interactorsGenome-scaleVesicle transportConserved proteinsChromosome segregationGene networksIdentified genesCellular processesPostembryonic rolesInteraction screenNuclear poresPleiotropic functionsGenesEarly embryosLarval development
2012
High-throughput fluorescence-based isolation of live C. elegans larvae
Fernandez A, Bargmann B, Mis E, Edgley M, Birnbaum K, Piano F. High-throughput fluorescence-based isolation of live C. elegans larvae. Nature Protocols 2012, 7: 1502-1510. PMID: 22814389, PMCID: PMC5274720, DOI: 10.1038/nprot.2012.084.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansFlow CytometryGreen Fluorescent ProteinsHigh-Throughput Screening AssaysHomozygoteLarvaConceptsGenetic interactionsChemical genetic screenNematode Caenorhabditis elegansFluorescence-activated cell sortingSelection of animalsTermination mutantsCaenorhabditis elegansSorting animalsGenetic screeningChemical screeningGFP expressionCell sortingLarval stageLive animalsHigh-speed sortingMutantsAnimalsFACSIsolatesGenotypesNematodesHomozygotes
2010
The Landscape of C. elegans 3′UTRs
Mangone M, Manoharan A, Thierry-Mieg D, Thierry-Mieg J, Han T, Mackowiak S, Mis E, Zegar C, Gutwein M, Khivansara V, Attie O, Chen K, Salehi-Ashtiani K, Vidal M, Harkins T, Bouffard P, Suzuki Y, Sugano S, Kohara Y, Rajewsky N, Piano F, Gunsalus K, Kim J. The Landscape of C. elegans 3′UTRs. Science 2010, 329: 432-435. PMID: 20522740, PMCID: PMC3142571, DOI: 10.1126/science.1191244.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAnimalsBinding SitesCaenorhabditis elegansComputational BiologyConserved SequenceDisorders of Sex DevelopmentGene Expression Regulation, DevelopmentalGene LibraryGenes, HelminthHelminth ProteinsHistonesMaleMicroRNAsOperonPoly APolyadenylationRNA, HelminthRNA, MessengerTrans-SplicingConceptsMetazoan messenger RNAsPolyadenylation signalComplementary DNAProtein-coding genesAmplification of complementary DNAMicroRNA target sitesVariant polyadenylation signalHistone genesGenome-widePolyadenylation sitesAlternative isoformsTrans-splicingGene modelsRNA-seqRegulatory elementsCaenorhabditis elegansLong 3'UTRUntranslated regionIsoform specificityPolyadenylationGenesMessenger RNATarget siteIsoformsAnimal ageAutomated sorting of live C. elegans using laFACS
Fernandez A, Mis E, Bargmann B, Birnbaum K, Piano F. Automated sorting of live C. elegans using laFACS. Nature Methods 2010, 7: 417-418. PMID: 20436474, PMCID: PMC2896029, DOI: 10.1038/nmeth.f.304.Peer-Reviewed Original Research