2004
Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension
Kahle KT, MacGregor GG, Wilson FH, Van Hoek AN, Brown D, Ardito T, Kashgarian M, Giebisch G, Hebert SC, Boulpaep EL, Lifton RP. Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 14877-14882. PMID: 15465913, PMCID: PMC522037, DOI: 10.1073/pnas.0406172101.Peer-Reviewed Original ResearchConceptsPseudohypoaldosteronism type IIPHAII-mutant WNK4Paracellular fluxPotent antihypertensive agentTight junction proteinsTight junctionsAntihypertensive agentsParacellular ion fluxPharmacologic propertiesTight junction structureTranscellular transportersWild-type WNK4Normal physiologyHypertensionTransepithelial resistanceWNK signalingKidney epitheliumTight junction formationParacellular pathwayWNK4Effect of WNK4EpitheliumType IIWNK4 kinaseHomeostasis
1999
Immunolocalization of the electrogenic Na+- HCO 3 − cotransporter in mammalian and amphibian kidney
Schmitt B, Biemesderfer D, Romero M, Boulpaep E, Boron W. Immunolocalization of the electrogenic Na+- HCO 3 − cotransporter in mammalian and amphibian kidney. American Journal Of Physiology 1999, 276: f27-f38. PMID: 9887077, DOI: 10.1152/ajprenal.1999.276.1.f27.Peer-Reviewed Original ResearchConceptsRat kidney NBCProximal tubulesDistal tubulesIndirect immunofluorescence microscopyEarly distal tubuleLate distal tubuleControl fusion proteinHCO-3 reabsorptionHCO-3 cotransporterRenal proximal tubulesBasolateral labelingImmunofluorescence microscopyRenal microsomesGuinea pigsRat kidneyHEK-293 cellsKidneyPreabsorption experimentsBasolateral stainingRabbit kidneyControl oocytesFusion proteinRatsProtein levelsNBC protein
1995
A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells
Korbmacher C, Volk T, Segal A, Boulpaep E, Frömter E. A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells. The Journal Of Membrane Biology 1995, 146: 29-45. PMID: 7563035, DOI: 10.1007/bf00232678.Peer-Reviewed Original ResearchConceptsSensitive nonselective cation channelNSC channelNonselective cation channelsDuct cellsAmiloride-sensitive sodium absorptionCation channelsChannel activityAdenine nucleotidesVoltage-dependent blockCalcium entryNonselective cation channel activitySodium absorptionCation channel activityCytoplasmic calciumInhibitory effectSpecial physiological conditionsCytoplasmic applicationMiceCGMP-dependent protein kinaseFlufenamic acidSingle-channel current recordingsMajor routeVolume regulationCurrent recordingsCGMP
1993
Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells.
Korbmacher C, Segal A, Fejes-Tóth G, Giebisch G, Boulpaep E. Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells. The Journal Of General Physiology 1993, 102: 761-793. PMID: 8270912, PMCID: PMC2229168, DOI: 10.1085/jgp.102.4.761.Peer-Reviewed Original ResearchConceptsWhole-cell currentsWhole-cell conductanceConfluent cellsWhole-cell membrane currentsBasolateral K channelsCells 24 hK conductancePA/cellMicroM glibenclamideAmiloride-sensitive conductanceTransgenic miceReabsorb sodiumOutward currentsM amilorideGlibenclamide inhibitionDuct cellsPrincipal cellsMembrane currentsBath KLarge depolarizationChloride conductanceK channelsLipophilic drugsPermeable supportsMice
1992
Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel.
Ahmad I, Korbmacher C, Segal A, Cheung P, Boulpaep E, Barnstable C. Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 10262-10266. PMID: 1279673, PMCID: PMC50318, DOI: 10.1073/pnas.89.21.10262.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCell LineCyclic GMPDNAIon Channel GatingIon ChannelsKidney CortexKidney Tubules, CollectingMembrane PotentialsMiceMolecular Sequence DataOligodeoxyribonucleotidesPhotoreceptor CellsPoly APolymerase Chain ReactionRatsRNARNA, MessengerSequence Homology, Nucleic AcidConceptsNonselective cation channelsPhotoreceptor channelDuct cellsCation channel probeCation channelsChannel activityPolymerase chain reactionNonselective cation channel activityCGMP decreaseCation channel activityChannel subunitsCytoplasmic applicationChain reactionRod photoreceptorsNorthern blot analysisRetinal cGMPBlot analysisCGMPRat kidney cDNA libraryChannel genesMiceSingle-channel conductanceOpen probabilityCellsCalcium removal