2005
Requirement of Voltage-Gated Calcium Channel ß4 Subunit for T Lymphocyte Functions
Badou A, Basavappa S, Desai R, Peng YQ, Matza D, Mehal WZ, Kaczmarek LK, Boulpaep EL, Flavell RA. Requirement of Voltage-Gated Calcium Channel ß4 Subunit for T Lymphocyte Functions. Science 2005, 307: 117-121. PMID: 15637280, DOI: 10.1126/science.1100582.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channels, L-TypeCalcium SignalingCD4-Positive T-LymphocytesCytokinesDNA-Binding ProteinsIon Channel GatingLymphocyte ActivationMembrane PotentialsMiceMice, Inbred C3HMice, Inbred C57BLMutationNFATC Transcription FactorsNuclear ProteinsPatch-Clamp TechniquesPhosphorylationProtein SubunitsReceptors, Antigen, T-CellT-LymphocytesTranscription FactorsConceptsT lymphocytesCalcium channelsVoltage-gated calcium channelsT lymphocyte functionT cell receptor stimulationCell receptor stimulationCytokine productionLymphocyte functionCalcium influxReceptor stimulationCalcium responseCalcium entryTranscription factor NFATCav1 channelsLymphocytesAlpha1 subunitCav channelsNormal functionNonexcitable cellsDisplay impairmentsExcitable cellsChannel openingMolecular identityDiverse physiological processesPhysiological processes
2003
Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells
Basavappa S, Mangel A, Boulpaep E. Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells. Biochemical And Biophysical Research Communications 2003, 308: 759-763. PMID: 12927783, DOI: 10.1016/s0006-291x(03)01481-5.Peer-Reviewed Original ResearchConceptsHuman neuroblastoma cell line CHP-100CHP-100Regulatory volume decreaseCell-attached patch-clamp studiesFree extracellular solutionHuman neuroblastoma cellsSwelling-activated ClPatch-clamp studiesFura-2Extracellular osmolarityFree extracellularNeuroblastoma cellsMOsm/Hypoosmotic solutionCell swellingExtracellular solutionCl- channelsHypoosmotic stressPresent studyMost mammalian cellsEGTACellsVolume decreaseEffluxPrevious studies
1998
Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 161-180. PMID: 9417142, PMCID: PMC1887764, DOI: 10.1085/jgp.111.1.161.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBiological TransportCalciumCell MembraneColforsinCyclic AMP-Dependent Protein KinasesEnzyme ActivationEpithelial CellsHydrogen-Ion ConcentrationIon Channel GatingKidney Tubules, ProximalPatch-Clamp TechniquesPhosphorylationPotassium ChannelsProtein Kinase CSodium-Potassium-Exchanging ATPaseConceptsBasolateral membraneProtein kinase AActin cytoskeletonATPase pumpOuter membraneProtein kinaseKinase ACellular metabolismKATP channelsSecond messengerStimulation of transportTransport activityPhysiological importanceMembrane stretchPhorbol esterChannel activityMembrane patchesRegulationIntracellularO patchesFunctional couplingHypotonic swellingCell-attached patchesCell depolarizationRenal proximal tubules
1995
A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells
Korbmacher C, Volk T, Segal A, Boulpaep E, Frömter E. A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells. The Journal Of Membrane Biology 1995, 146: 29-45. PMID: 7563035, DOI: 10.1007/bf00232678.Peer-Reviewed Original ResearchConceptsSensitive nonselective cation channelNSC channelNonselective cation channelsDuct cellsAmiloride-sensitive sodium absorptionCation channelsChannel activityAdenine nucleotidesVoltage-dependent blockCalcium entryNonselective cation channel activitySodium absorptionCation channel activityCytoplasmic calciumInhibitory effectSpecial physiological conditionsCytoplasmic applicationMiceCGMP-dependent protein kinaseFlufenamic acidSingle-channel current recordingsMajor routeVolume regulationCurrent recordingsCGMP
1993
Endothelin increases [Ca2+]i in M-1 mouse cortical collecting duct cells by a dual mechanism
Korbmacher C, Boulpaep E, Giebisch G, Geibel J. Endothelin increases [Ca2+]i in M-1 mouse cortical collecting duct cells by a dual mechanism. American Journal Of Physiology 1993, 265: c349-c357. PMID: 8368264, DOI: 10.1152/ajpcell.1993.265.2.c349.Peer-Reviewed Original ResearchConceptsEndothelin-1Intracellular storesDuct cellsNifedipine-sensitive Ca2Intracellular calcium concentrationParticipation of Ca2Endothelin increaseFura-2Extracellular calciumExtracellular Ca2Arginine vasopressinIntracellular Ca2Calcium concentrationInitial Ca2Fluorescent imaging systemInitial exposurePlateau phaseMiceEntry mechanismDual mechanismCa2Second Ca2CellsNifedipineVasopressin
1984
Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.
Hunter M, Lopes A, Boulpaep E, Giebisch G. Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 4237-4239. PMID: 6330754, PMCID: PMC345404, DOI: 10.1073/pnas.81.13.4237.Peer-Reviewed Original ResearchConceptsPotassium channelsRabbit cortical collecting tubuleCalcium concentrationCalcium-activated potassium channelsDose-dependent fashionCortical collecting tubulePatch-clamp techniqueCollecting tubuleSingle potassium channelsApical membraneOpen channel probabilitySingle-channel recordingsSlope conductanceChannel activityTubulesChannel recordingsRecordings