2003
Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon
Joiner WJ, Basavappa S, Vidyasagar S, Nehrke K, Krishnan S, Binder HJ, Boulpaep EL, Rajendran VM. Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon. AJP Gastrointestinal And Liver Physiology 2003, 285: g185-g196. PMID: 12606302, DOI: 10.1152/ajpgi.00337.2002.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsApaminCarbacholChloridesCHO CellsCholinergic AgonistsClotrimazoleColonCricetinaeGene ExpressionGrowth InhibitorsIntermediate-Conductance Calcium-Activated Potassium ChannelsMaleMembrane PotentialsMolecular Sequence DataPatch-Clamp TechniquesPeptidesPotassiumPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium, DietaryRatsRats, Sprague-DawleyRubidium RadioisotopesConceptsChannel inhibitors clotrimazoleRat proximal colonProximal colonSK channel inhibitorsAddition of thapsigarginExpression of Ca2Muscarinic receptorsCholinergic agentsMuscarinic agonistsEpithelial stripsIKCa channelsMucosal additionSerosal surfaceColonic epitheliumIK channelsIntracellular Ca2Channel inhibitorsRSK4 mRNAColonWestern blottingIK proteinSecretionColonic cryptsPrecise mechanismConfocal immunofluorescence
1997
Expression cloning and characterization of a renal electrogenic Na+ /HCO3− cotransporter
Romero M, Hediger M, Boulpaep E, Boron W. Expression cloning and characterization of a renal electrogenic Na+ /HCO3− cotransporter. Nature 1997, 387: 409-413. PMID: 9163427, DOI: 10.1038/387409a0.Peer-Reviewed Original ResearchConceptsPotential membrane-spanning domainsMembrane-spanning domainsElectrogenic sodium bicarbonate cotransporterBinding of inhibitorsSodium bicarbonate cotransporterAnimal cellsBicarbonate transportersElectrogenic transportersSuccessful cloningExpression cloningComplementary DNAPrincipal regulatorAmino acidsBicarbonate cotransporterProtein purificationFunctional familiesXenopus oocytesMolecular informationTransportersCloningReproductive systemSalamander Ambystoma tigrinumProteinAmbystoma tigrinumIntracellular pH