1999
Immunolocalization of the electrogenic Na+- HCO 3 − cotransporter in mammalian and amphibian kidney
Schmitt B, Biemesderfer D, Romero M, Boulpaep E, Boron W. Immunolocalization of the electrogenic Na+- HCO 3 − cotransporter in mammalian and amphibian kidney. American Journal Of Physiology 1999, 276: f27-f38. PMID: 9887077, DOI: 10.1152/ajprenal.1999.276.1.f27.Peer-Reviewed Original ResearchConceptsRat kidney NBCProximal tubulesDistal tubulesIndirect immunofluorescence microscopyEarly distal tubuleLate distal tubuleControl fusion proteinHCO-3 reabsorptionHCO-3 cotransporterRenal proximal tubulesBasolateral labelingImmunofluorescence microscopyRenal microsomesGuinea pigsRat kidneyHEK-293 cellsKidneyPreabsorption experimentsBasolateral stainingRabbit kidneyControl oocytesFusion proteinRatsProtein levelsNBC protein
1997
The electrogenic Na/HCO3 cotransporter.
Boron W, Fong P, Hediger M, Boulpaep E, Romero M. The electrogenic Na/HCO3 cotransporter. Wiener Klinische Wochenschrift 1997, 109: 445-56. PMID: 9261985.Peer-Reviewed Original ResearchConceptsElectrogenic Na/HCO3 cotransporterProximal tubulesHCO3- cotransporterRenal proximal tubulesCO2/10 mM HCO3HCO3- transportersRegulation of intracellularBasolateral membraneNumber of tissuesNative oocytesRapid hyperpolarizationXenopus laevis oocytesExtracellular ClBicarbonate cotransporterOocyte expressionCotransporterMM HCO3OocytesCell typesMost cellsMembrane potentialAmino acid homologySingle cloneWater-injected controlsTubulesThe Renal Electrogenic Na+:HCO3− Cotransporter
Boron W, Hediger M, Boulpaep E, Romero M. The Renal Electrogenic Na+:HCO3− Cotransporter. Journal Of Experimental Biology 1997, 200: 263-268. PMID: 9050234, DOI: 10.1242/jeb.200.2.263.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsBicarbonatesCarrier ProteinsCloning, MolecularDNA, ComplementaryHydrogen-Ion ConcentrationKidney Tubules, ProximalMembrane PotentialsMicroinjectionsOocytesRabbitsRecombinant Fusion ProteinsRNA, MessengerSodiumSodium-Bicarbonate SymportersStructure-Activity RelationshipXenopus laevisConceptsRenal proximal tubules
1996
Molecular cloning of a glibenclamide-sensitive, voltage-gated potassium channel expressed in rabbit kidney.
Yao X, Chang AY, Boulpaep EL, Segal AS, Desir GV. Molecular cloning of a glibenclamide-sensitive, voltage-gated potassium channel expressed in rabbit kidney. Journal Of Clinical Investigation 1996, 97: 2525-2533. PMID: 8647945, PMCID: PMC507338, DOI: 10.1172/jci118700.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBrainCloning, MolecularDNA PrimersFemaleGenetic VariationGenomic LibraryGlyburideHumansKidney MedullaKv1.3 Potassium ChannelMiceModels, BiologicalMolecular Sequence DataOocytesPancreatitis-Associated ProteinsPhylogenyPolymerase Chain ReactionPotassium ChannelsPotassium Channels, Voltage-GatedRabbitsRecombinant ProteinsSequence Homology, Amino AcidXenopus laevisConceptsVoltage-gated potassium channelsMolecular cloningFunctional expressionShaker-like potassium channelsPotassium channelsShaker geneGRB-PAP1Novel memberAmino terminusMolecular evidenceShaker channelsAmino acidsXenopus oocytesRabbit kidneyRenal potassium transportCloningGenesPotassium transportChannel clonesFirst reportRabbit brainPotassium conductanceFamilyExpressionKidney
1995
Primary structure and functional expression of a cGMP-gated potassium channel.
Yao X, Segal AS, Welling P, Zhang X, McNicholas CM, Engel D, Boulpaep EL, Desir GV. Primary structure and functional expression of a cGMP-gated potassium channel. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 11711-11715. PMID: 8524834, PMCID: PMC40472, DOI: 10.1073/pnas.92.25.11711.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCloning, MolecularCyclic GMPElectric ConductivityGene LibraryIon Channel GatingMolecular Sequence DataOocytesPhylogenyPotassiumPotassium ChannelsProtein BiosynthesisProtein ConformationRabbitsRNA, MessengerSequence Homology, Amino AcidTissue DistributionXenopusConceptsDeduced amino acid sequenceChannel protein phosphorylationCysteine-rich regionAmino acid sequenceNorthern blot analysisPotassium channel activityProtein phosphorylationAcid sequenceGene expressionPrimary structureFunctional expressionK channelsIon channelsChannel activityBlot analysisCyclic nucleotidesShaker K channelsPotassium channelsEffects of substancesKCN1Intracellular cGMPCGMPNitric oxideExpressionImportant role
1989
MECHANISMS OF WATER TRANSPORT BY EPITHELIAL CELLS
Tripathi S, Boulpaep E. MECHANISMS OF WATER TRANSPORT BY EPITHELIAL CELLS. Quarterly Journal Of Experimental Physiology And Cognate Medical Sciences 1989, 74: 385-417. PMID: 2678220, DOI: 10.1113/expphysiol.1989.sp003288.Peer-Reviewed Original Research
1986
Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule
Hunter M, Lopes A, Boulpaep E, Giebisch G. Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule. American Journal Of Physiology 1986, 251: f725-f733. PMID: 2429562, DOI: 10.1152/ajprenal.1986.251.4.f725.Peer-Reviewed Original ResearchConceptsCortical collecting tubuleCollecting tubuleRabbit cortical collecting tubuleBath calcium concentrationOpen probabilityPatch-clamp techniqueOne-half maximalApical membranePotassium secretionChannel open timeIntracellular calciumPotassium ion channelsChannel open probabilityCalcium concentrationMM calciumChannel activityTubulesIon channelsBath solutionSecretionOpen timeTransmembrane potentialCalciumFunction of calciumApparent Ki
1984
Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.
Hunter M, Lopes A, Boulpaep E, Giebisch G. Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 4237-4239. PMID: 6330754, PMCID: PMC345404, DOI: 10.1073/pnas.81.13.4237.Peer-Reviewed Original ResearchConceptsPotassium channelsRabbit cortical collecting tubuleCalcium concentrationCalcium-activated potassium channelsDose-dependent fashionCortical collecting tubulePatch-clamp techniqueCollecting tubuleSingle potassium channelsApical membraneOpen channel probabilitySingle-channel recordingsSlope conductanceChannel activityTubulesChannel recordingsRecordings
1982
Ionic conductive properties and electrophysiology of the rabbit cortical collecting tubule
O'Neil R, Boulpaep E. Ionic conductive properties and electrophysiology of the rabbit cortical collecting tubule. American Journal Of Physiology 1982, 243: f81-f95. PMID: 7091368, DOI: 10.1152/ajprenal.1982.243.1.f81.Peer-Reviewed Original Research
1979
Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium
O'Neil R, Boulpaep E. Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium. The Journal Of Membrane Biology 1979, 50: 365-387. PMID: 513119, DOI: 10.1007/bf01868898.Peer-Reviewed Original ResearchConceptsLumen-negative transepithelial potential differenceActive Na absorptionApical cell membraneEffects of amilorideEquivalent short-circuit currentTransepithelial potential differenceNa absorptionMaximum inhibitory concentrationAmilorideDiuretic amilorideCation channelsCell membraneNa channelsInhibitory concentrationRenal epitheliumMembrane cation channelsRemoval of NaAbsence of NaTight junctionsPerfusateK permeabilityTubulesLumenModulation of cell membrane area in renal collecting tubules by corticosteroid hormones.
Wade J, O'Neil R, Pryor J, Boulpaep E. Modulation of cell membrane area in renal collecting tubules by corticosteroid hormones. Journal Of Cell Biology 1979, 81: 439-445. PMID: 468913, PMCID: PMC2110313, DOI: 10.1083/jcb.81.2.439.Peer-Reviewed Original Research
1971
Electrolyte transport in kidney tubule cells
Giebisch G, Boulpaep E, Whittembury G. Electrolyte transport in kidney tubule cells. Philosophical Transactions Of The Royal Society B Biological Sciences 1971, 262: 175-196. PMID: 4399217, DOI: 10.1098/rstb.1971.0088.Peer-Reviewed Original ResearchConceptsTubule cellsTubular levelKidney tubule cellsNet Na transportActive K uptakePotassium secretionNa-K exchange pumpK secretionProximal tubular levelLuminal cell membraneElectrolyte transportNa pumpLines of evidenceTranstubular movementNa transportNet NaCell compartmentExchange pumpSecretionInterstitial fluidCellsLumenPassive leakIonic pumpsUptake