2003
Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon
Joiner WJ, Basavappa S, Vidyasagar S, Nehrke K, Krishnan S, Binder HJ, Boulpaep EL, Rajendran VM. Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon. AJP Gastrointestinal And Liver Physiology 2003, 285: g185-g196. PMID: 12606302, DOI: 10.1152/ajpgi.00337.2002.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsApaminCarbacholChloridesCHO CellsCholinergic AgonistsClotrimazoleColonCricetinaeGene ExpressionGrowth InhibitorsIntermediate-Conductance Calcium-Activated Potassium ChannelsMaleMembrane PotentialsMolecular Sequence DataPatch-Clamp TechniquesPeptidesPotassiumPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium, DietaryRatsRats, Sprague-DawleyRubidium RadioisotopesConceptsChannel inhibitors clotrimazoleRat proximal colonProximal colonSK channel inhibitorsAddition of thapsigarginExpression of Ca2Muscarinic receptorsCholinergic agentsMuscarinic agonistsEpithelial stripsIKCa channelsMucosal additionSerosal surfaceColonic epitheliumIK channelsIntracellular Ca2Channel inhibitorsRSK4 mRNAColonWestern blottingIK proteinSecretionColonic cryptsPrecise mechanismConfocal immunofluorescence
1992
Differentiated Transport Properties of Primary Cultured Distal Nephron
White S, Boulpaep E, Giebisch G. Differentiated Transport Properties of Primary Cultured Distal Nephron. Cellular Physiology And Biochemistry 1992, 2: 323-335. DOI: 10.1159/000154655.Peer-Reviewed Original ResearchAcid secretionNet acid secretionEquivalent short-circuit currentIncubation of monolayersRabbit kidney cortexCollagen-coated permeable supportsDistal nephronPositive cellsKidney cortexPercoll density centrifugationNephron fragmentsBasolateral fluidInsensitive componentsSecretionTransport of sodiumBasolateral solutionPermeable supportsH incubationDensity centrifugationCellsAldosteroneCulture mediumCortexRich cellsNephron
1986
Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule
Hunter M, Lopes A, Boulpaep E, Giebisch G. Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule. American Journal Of Physiology 1986, 251: f725-f733. PMID: 2429562, DOI: 10.1152/ajprenal.1986.251.4.f725.Peer-Reviewed Original ResearchConceptsCortical collecting tubuleCollecting tubuleRabbit cortical collecting tubuleBath calcium concentrationOpen probabilityPatch-clamp techniqueOne-half maximalApical membranePotassium secretionChannel open timeIntracellular calciumPotassium ion channelsChannel open probabilityCalcium concentrationMM calciumChannel activityTubulesIon channelsBath solutionSecretionOpen timeTransmembrane potentialCalciumFunction of calciumApparent Ki
1971
Electrolyte transport in kidney tubule cells
Giebisch G, Boulpaep E, Whittembury G. Electrolyte transport in kidney tubule cells. Philosophical Transactions Of The Royal Society B Biological Sciences 1971, 262: 175-196. PMID: 4399217, DOI: 10.1098/rstb.1971.0088.Peer-Reviewed Original ResearchConceptsTubule cellsTubular levelKidney tubule cellsNet Na transportActive K uptakePotassium secretionNa-K exchange pumpK secretionProximal tubular levelLuminal cell membraneElectrolyte transportNa pumpLines of evidenceTranstubular movementNa transportNet NaCell compartmentExchange pumpSecretionInterstitial fluidCellsLumenPassive leakIonic pumpsUptake