1997
Tubule electrophysiology: from single channels back to the renal epithelium.
Boulpaep E. Tubule electrophysiology: from single channels back to the renal epithelium. Wiener Klinische Wochenschrift 1997, 109: 489-92. PMID: 9261991.Peer-Reviewed Original ResearchConceptsMacroscopic membrane propertiesMicroscopic parametersSingle channel proteinsElectrical propertiesIon flowMacroscopic referenceSingle barrierMembrane patchesRenal epitheliumSingle channelRenal ion transportRenal ion channelsRegulatory domainExperimental errorChannel proteinsPore conductivityIon channelsWhole-cell conductancePatch-clamp technologyBasolateral membraneRenal tubule cellsCell membrane parametersIon selectivityIon transportOpen probability
1992
Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel.
Ahmad I, Korbmacher C, Segal A, Cheung P, Boulpaep E, Barnstable C. Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 10262-10266. PMID: 1279673, PMCID: PMC50318, DOI: 10.1073/pnas.89.21.10262.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCell LineCyclic GMPDNAIon Channel GatingIon ChannelsKidney CortexKidney Tubules, CollectingMembrane PotentialsMiceMolecular Sequence DataOligodeoxyribonucleotidesPhotoreceptor CellsPoly APolymerase Chain ReactionRatsRNARNA, MessengerSequence Homology, Nucleic AcidConceptsNonselective cation channelsPhotoreceptor channelDuct cellsCation channel probeCation channelsChannel activityPolymerase chain reactionNonselective cation channel activityCGMP decreaseCation channel activityChannel subunitsCytoplasmic applicationChain reactionRod photoreceptorsNorthern blot analysisRetinal cGMPBlot analysisCGMPRat kidney cDNA libraryChannel genesMiceSingle-channel conductanceOpen probabilityCellsCalcium removal
1986
Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule
Hunter M, Lopes A, Boulpaep E, Giebisch G. Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule. American Journal Of Physiology 1986, 251: f725-f733. PMID: 2429562, DOI: 10.1152/ajprenal.1986.251.4.f725.Peer-Reviewed Original ResearchConceptsCortical collecting tubuleCollecting tubuleRabbit cortical collecting tubuleBath calcium concentrationOpen probabilityPatch-clamp techniqueOne-half maximalApical membranePotassium secretionChannel open timeIntracellular calciumPotassium ion channelsChannel open probabilityCalcium concentrationMM calciumChannel activityTubulesIon channelsBath solutionSecretionOpen timeTransmembrane potentialCalciumFunction of calciumApparent Ki