1992
Differentiated Transport Properties of Primary Cultured Distal Nephron
White S, Boulpaep E, Giebisch G. Differentiated Transport Properties of Primary Cultured Distal Nephron. Cellular Physiology And Biochemistry 1992, 2: 323-335. DOI: 10.1159/000154655.Peer-Reviewed Original ResearchAcid secretionNet acid secretionEquivalent short-circuit currentIncubation of monolayersRabbit kidney cortexCollagen-coated permeable supportsDistal nephronPositive cellsKidney cortexPercoll density centrifugationNephron fragmentsBasolateral fluidInsensitive componentsSecretionTransport of sodiumBasolateral solutionPermeable supportsH incubationDensity centrifugationCellsAldosteroneCulture mediumCortexRich cellsNephron
1983
Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium
Guggino W, London R, Boulpaep E, Giebisch G. Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium. The Journal Of Membrane Biology 1983, 71: 227-240. PMID: 6302263, DOI: 10.1007/bf01875464.Peer-Reviewed Original ResearchConceptsBasolateral cell membraneProximal tubule cellsBasolateral solutionNH4Cl exposurePH-sensitive microelectrodesProximal tubulesTubule cellsPerfusion solutionCell membraneIntracellular chlorideTheNecturus proximal tubuleIntracellular pHApical cell membraneNH4Cl treatmentChloride transportChloride activityKidneyIntracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. The Journal Of General Physiology 1983, 81: 29-52. PMID: 6833996, PMCID: PMC2215563, DOI: 10.1085/jgp.81.1.29.Peer-Reviewed Original ResearchConceptsProximal tubulesProximal tubule cellsRenal proximal tubulesTiger salamander Ambystoma tigrinumPH-sensitive microelectrodesFree RingerMaximal effectTubule cellsTransient riseDiuretic amilorideBasolateral sideBasolateral solutionExponential time courseSalamander Ambystoma tigrinumTime courseCl removalMaximal rateBasolateral membraneNormal rateTubulesAmbystoma tigrinumPH regulationCellsActive transportRecovery