2004
Type III Secretion System Genes in Clinical Aeromonas Isolates
Chacón MR, Soler L, Groisman EA, Guarro J, Figueras MJ. Type III Secretion System Genes in Clinical Aeromonas Isolates. Journal Of Clinical Microbiology 2004, 42: 1285-1287. PMID: 15004096, PMCID: PMC356863, DOI: 10.1128/jcm.42.3.1285-1287.2004.Peer-Reviewed Original ResearchMeSH KeywordsAeromonasAmino Acid SequenceBase SequenceDNA PrimersGenes, BacterialGram-Negative Bacterial InfectionsHumansMolecular Sequence DataSequence AlignmentSequence Homology, Amino AcidVirulence
2003
Signal-dependent Requirement for the Co-activator Protein RcsA in Transcription of the RcsB-regulated ugd Gene*
Mouslim C, Latifi T, Groisman EA. Signal-dependent Requirement for the Co-activator Protein RcsA in Transcription of the RcsB-regulated ugd Gene*. Journal Of Biological Chemistry 2003, 278: 50588-50595. PMID: 14514676, DOI: 10.1074/jbc.m309433200.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBase SequenceBeta-GalactosidaseBinding SitesChromosomesDeoxyribonuclease IEscherichia coli ProteinsGene DeletionIronMagnesiumModels, BiologicalModels, GeneticMolecular Sequence DataMutationPeriplasmic ProteinsPlasmidsPromoter Regions, GeneticSalmonellaSequence Homology, Amino AcidSingle-Strand Specific DNA and RNA EndonucleasesTemperatureTranscription FactorsTranscription, GeneticConceptsUgd genePmrA/PmrB systemTwo-component systems PhoP/PhoQS1 mapping experimentsPhoP/PhoQPmrA/PmrBUDP-glucose dehydrogenasePhoP proteinPhosphorelay systemIndependent transcriptionVariety of signalsPmrA mutantMembrane proteinsSame promoterCps operonRcsBGene expressionRcsATranscriptionGenesMapping experimentsCps transcriptionPhoPPromoterLow Mg2The Salmonella SpiC protein targets the mammalian Hook3 protein function to alter cellular trafficking
Shotland Y, Krämer H, Groisman EA. The Salmonella SpiC protein targets the mammalian Hook3 protein function to alter cellular trafficking. Molecular Microbiology 2003, 49: 1565-1576. PMID: 12950921, DOI: 10.1046/j.1365-2958.2003.03668.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBacterial ProteinsCell LineCyclic AMPGenes, ReporterLysosomesMacrophagesMiceMicroscopy, FluorescenceMicrotubule-Associated ProteinsMolecular Sequence DataPhagosomesProtein BindingProtein TransportRecombinant Fusion ProteinsSalmonella typhimuriumSequence Homology, Amino AcidConceptsPhagosome-lysosome fusionCellular traffickingType III secretion systemEndosome-endosome fusionDominant negative mutantCytosol of macrophagesMammalian proteinsPhenotype of cellsProtein functionGolgi morphologySecretion systemDistribution of lysosomesSpiC genePhagosomal membraneProteinMurine macrophagesTraffickingCytosolVero cellsMacrophagesCellsMutantsHook3FusionGenesMg2+ Sensing by the Mg2+ Sensor PhoQ of Salmonella enterica
Chamnongpol S, Cromie M, Groisman EA. Mg2+ Sensing by the Mg2+ Sensor PhoQ of Salmonella enterica. Journal Of Molecular Biology 2003, 325: 795-807. PMID: 12507481, DOI: 10.1016/s0022-2836(02)01268-8.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid SubstitutionBacterial ProteinsBase SequenceBinding SitesConserved SequenceDNA, BacterialMagnesiumMolecular Sequence DataMutagenesis, Site-DirectedPhosphorylationProtein Structure, TertiaryRecombinant ProteinsSalmonella entericaSequence Homology, Amino AcidTranscription, GeneticConceptsTranscription of PhoPPhoQ proteinSensor PhoQPeriplasmic domainTwo-component regulatory systemResponse regulator PhoPExpression of phoPPhoP/PhoQWild-type responseWild-type abilityAmino acid residuesGram-negative speciesRegulator PhoPGene transcriptionPhoPAcid residuesTranscriptionHistidine residuesPhoQGenesAcetyl phosphateRegulatory systemProteinMutantsSalmonella enterica
1996
Identification of a pathogenicity island required for Salmonella survival in host cells.
Ochman H, Soncini FC, Solomon F, Groisman EA. Identification of a pathogenicity island required for Salmonella survival in host cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 7800-7804. PMID: 8755556, PMCID: PMC38828, DOI: 10.1073/pnas.93.15.7800.Peer-Reviewed Original ResearchConceptsPathogenicity islandType III secretion systemSalmonella typhimurium chromosomeWild-type levelsEpithelial cell invasionWild-type strainSPI genesSecretion systemPutative regulatorHost cellsSecretory apparatusCell invasionSecreted proteaseVirulence determinantsSpi(-) mutantsGenesProteinProteaseSalmonella survivalEnteric pathogensMutantsChromosomesIslandsCulture supernatantsForms of flagellin
1994
A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium.
Parra‐Lopez C, Lin R, Aspedon A, Groisman EA. A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium. The EMBO Journal 1994, 13: 3964-3972. PMID: 8076592, PMCID: PMC395316, DOI: 10.1002/j.1460-2075.1994.tb06712.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceBiological TransportCarrier ProteinsCloning, MolecularDrug Resistance, MicrobialMelittenMembrane ProteinsMolecular Sequence DataNADPeptidesPotassiumProtaminesReceptor, trkARecombinant ProteinsRestriction MappingSalmonella typhimuriumSequence Analysis, DNASequence Homology, Amino AcidConceptsE. coli proteinsAntimicrobial peptidesMolecular genetic analysisAntimicrobial peptide protaminePutative transportersTransport of peptidesColi proteinsSingle mutantsSalmonella proteinsSame resistance pathwaysSAP mutantsHost defense moleculesGenetic analysisDefense moleculesLoci participateChannel proteinsExhibit hypersensitivityEfflux proteinsUptake systemResistance pathwaysMutantsEscherichia coliProteinTransport of potassiumHost tissues
1993
Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium.
Parra‐Lopez C, Baer MT, Groisman EA. Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium. The EMBO Journal 1993, 12: 4053-4062. PMID: 8223423, PMCID: PMC413698, DOI: 10.1002/j.1460-2075.1993.tb06089.x.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceAnti-Bacterial AgentsATP-Binding Cassette TransportersBase SequenceBiological TransportCloning, MolecularDrug Resistance, MicrobialEnterobacteriaceaeGenes, BacterialMelittenModels, BiologicalMolecular Sequence DataOperonPeptidesProtaminesSalmonella typhimuriumSequence Analysis, DNASequence Homology, Amino AcidConceptsUptake of oligopeptidesSet of genesOpen reading frameMolecular genetic analysisMammalian mdrYeast STE6Wild-type plasmidOperon structurePeriplasmic componentPeptide pheromoneNovel transporterReading frameKb segmentSalmonella typhimuriumGenetic analysisKb mRNASmall cationic peptidesSuccessful pathogenAntimicrobial peptide melittinSequence analysisCassette familyEnteric bacteriaPeptide transportAntimicrobial peptidesCancer cellsCognate gene clusters govern invasion of host epithelial cells by Salmonella typhimurium and Shigella flexneri.
Groisman EA, Ochman H. Cognate gene clusters govern invasion of host epithelial cells by Salmonella typhimurium and Shigella flexneri. The EMBO Journal 1993, 12: 3779-3787. PMID: 8404849, PMCID: PMC413660, DOI: 10.1002/j.1460-2075.1993.tb06056.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, BacterialAntigens, SurfaceBase SequenceBlotting, SouthernChromosomes, BacterialDNAEpithelial CellsEpitheliumGenes, BacterialGenetic Complementation TestHumansMiceMolecular Sequence DataMultigene FamilyMutationRestriction MappingSalmonella typhimuriumSequence Homology, Amino AcidShigella flexneriTumor Cells, CulturedConceptsEpithelial cellsEnteric pathogen Salmonella typhimuriumHost epithelial cellsSurface antigenSalmonella typhimuriumPathogen Salmonella typhimuriumConstellation of genesInvasion propertiesShigella flexneriVirulence plasmidShigellaVirulence attributesAntigenSalmonellaBacterial entryProtein secretionMolecular, functional, and evolutionary analysis of sequences specific to Salmonella.
Groisman EA, Sturmoski MA, Solomon FR, Lin R, Ochman H. Molecular, functional, and evolutionary analysis of sequences specific to Salmonella. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 1033-1037. PMID: 8430070, PMCID: PMC45805, DOI: 10.1073/pnas.90.3.1033.Peer-Reviewed Original ResearchConceptsAtypical base compositionIndependent evolutionary eventsPhenotypic characteristicsOpen reading frameLac gene fusionsWild-type parentLysR familyEvolutionary eventsDeletion strainEvolutionary analysisTranscriptional regulatorsReading frameSalmonella genomeNucleotide sequenceBase compositionHorizontal transferDNA fragmentsGene fusionsBacterial speciesEnteric bacteriaStructural similaritySpeciesVirulenceSequenceUnprecedented array