2017
Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate
Yeom J, Wayne KJ, Groisman EA. Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate. Molecular Cell 2017, 66: 234-246.e5. PMID: 28431231, PMCID: PMC5424706, DOI: 10.1016/j.molcel.2017.03.009.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacterial ProteinsBinding, CompetitiveCation Transport ProteinsEndopeptidase ClpGene Expression Regulation, BacterialHalf-LifeModels, MolecularMutationProtein BindingProtein Interaction Domains and MotifsProtein StabilityProteolysisSalmonella typhimuriumStructure-Activity RelationshipSubstrate SpecificityTime FactorsTranscription, GeneticConceptsN-terminal residuesSpecific N-terminal residuesRegulatory protein PhoPN-end ruleProtease ClpAPTemporal transcriptionMgtC geneN-terminusPhoPSpecific substratesClpAPDifferential stabilityProtein levelsGenesMgtCResiduesTranscriptionTerminusProteolysisProteinSubstrateProteaseBacteriaDegradationSequestration
2014
Bacterial Nucleoid-Associated Protein Uncouples Transcription Levels from Transcription Timing
Zwir I, Yeo WS, Shin D, Latifi T, Huang H, Groisman EA. Bacterial Nucleoid-Associated Protein Uncouples Transcription Levels from Transcription Timing. MBio 2014, 5: 10.1128/mbio.01485-14. PMID: 25293763, PMCID: PMC4196223, DOI: 10.1128/mbio.01485-14.Peer-Reviewed Original ResearchConceptsTranscription timingPhoP proteinAncestral geneExpression timingTranscriptional regulatorsGene ancestryTranscription factorsTranscription levelsHistone-like nucleoid structuring proteinHigher mRNA levelsExpression behaviorH-NS proteinNucleoid structuring proteinBacterium Salmonella enterica serovar TyphimuriumExpression of genesSalmonella enterica serovar TyphimuriumH-NSLatter genesEnterica serovar TyphimuriumMRNA levelsCorresponding promotersTarget genesDevelopmental pathwaysEarly genesGenes
2013
Signal‐specific temporal response by the Salmonella PhoP/PhoQ regulatory system
Park S, Groisman EA. Signal‐specific temporal response by the Salmonella PhoP/PhoQ regulatory system. Molecular Microbiology 2013, 91: 135-144. PMID: 24256574, PMCID: PMC3890429, DOI: 10.1111/mmi.12449.Peer-Reviewed Original ResearchConceptsTransporter MgtATwo-component systems PhoP/PhoQPhoP-dependent genesAntimicrobial peptide C18G.PhoP/PhoQSalmonella enterica serovar TyphimuriumSensor PhoQEnterica serovar TyphimuriumVirulence functionsRepressing signalsGene setsPhoQMgtAGenesSerovar TyphimuriumElicit expressionPeriplasmPhoPActive formFull transcriptionExpressionPhoQ.TranscriptionCytoplasmicProtein
1996
The antibacterial action of protamine: evidence for disruption of cytoplasmic membrane energization in Salmonella typhimurium
Aspedon A, Groisman EA. The antibacterial action of protamine: evidence for disruption of cytoplasmic membrane energization in Salmonella typhimurium. Microbiology 1996, 142: 3389-3397. PMID: 9004502, DOI: 10.1099/13500872-142-12-3389.Peer-Reviewed Original ResearchConceptsCytoplasmic membraneNuclei of spermNutrient uptake functionsCellular ATP contentElectrical membrane potentialHigher delta psi valuesRespiring cellsLoss of viabilityMembrane energizationDifferent animal speciesProline uptakeProtein synthesisEnergy transductionMode of actionAnimal speciesUptake functionDelta psi valuesMembrane potentialCell lysisRapid effluxMechanism of actionPolycationic peptidesATP contentProtamineSalmonella typhimurium