2009
Evolution of a Bacterial Regulon Controlling Virulence and Mg2+ Homeostasis
Perez JC, Shin D, Zwir I, Latifi T, Hadley TJ, Groisman EA. Evolution of a Bacterial Regulon Controlling Virulence and Mg2+ Homeostasis. PLOS Genetics 2009, 5: e1000428. PMID: 19300486, PMCID: PMC2650801, DOI: 10.1371/journal.pgen.1000428.Peer-Reviewed Original ResearchConceptsRegulatory proteinsSpecies-specific traitsHorizontal gene transferDifferent gene setsSpecies-specific targetsPhoP proteinTranscriptional rewiringPhoP regulonRelated organismsPhoPQ operonGene setsVariable membersCell envelopeProtein activityRegulonBacterial speciesGene transferSpeciesProteinPhoPFamily EnterobacteriaceaeGenesVirulenceHomeostasisWidespread effects
2000
A parallel intraphagosomal survival strategy shared by Mycobacterium tuberculosis and Salmonella enterica
Buchmeier N, Blanc‐Potard A, Ehrt S, Piddington D, Riley L, Groisman E. A parallel intraphagosomal survival strategy shared by Mycobacterium tuberculosis and Salmonella enterica. Molecular Microbiology 2000, 35: 1375-1382. PMID: 10760138, DOI: 10.1046/j.1365-2958.2000.01797.x.Peer-Reviewed Original ResearchConceptsM. tuberculosisIntracellular pathogensMycobacterium tuberculosisSpleens of miceCultured human macrophagesM. tuberculosis mutantsMgtC mutantMacrophage phagosomesTuberculosisSalmonella entericaHuman macrophagesTuberculosis mutantsMacrophagesDifferent diseasesLow magnesiumVirulencePhagosomesMgtC proteinSimilar phenotypeSalmonellaPrevious studiesPathogensLungSpleen
1999
The selC‐associated SHI‐2 pathogenicity island of Shigella flexneri
Moss J, Cardozo T, Zychlinsky A, Groisman E. The selC‐associated SHI‐2 pathogenicity island of Shigella flexneri. Molecular Microbiology 1999, 33: 74-83. PMID: 10411725, DOI: 10.1046/j.1365-2958.1999.01449.x.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsChromosome MappingChromosomes, BacterialCloning, MolecularColicinsDNA Transposable ElementsDNA, BacterialEnterobacteriaceaeEscherichia coliGenes, BacterialHydroxamic AcidsIntegrasesOperonSequence AlignmentSequence Homology, Nucleic AcidShigella flexneriSpecies SpecificityTransposasesVirulenceConceptsPathogenicity islandSHI-2Different pathogenicity islandsChromosomal gene clusterRelated non-pathogenic speciesMultiple mobile genetic elementsMobile genetic elementsNon-pathogenic speciesSite of integrationTRNA genesSelC locusTRNA locusGene clusterNovel proteinGenetic elementsKb downstreamSiderophore systemStressful environmentsEscherichia coliVirulence factorsEnterohaemorrhagic E. coliE. coliUropathogenic Escherichia coliSalmonella entericaGenes
1994
How to become a pathogen
Groisman E, Ochman H. How to become a pathogen. Trends In Microbiology 1994, 2: 289-294. PMID: 7981972, DOI: 10.1016/0966-842x(94)90006-x.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsGenes, BacterialGenes, SuppressorMiceSalmonellaSpecies SpecificityVirulenceThe origin and evolution of species differences in Escherichia coli and Salmonella typhimurium
Ochman H, Groisman EA. The origin and evolution of species differences in Escherichia coli and Salmonella typhimurium. EXS 1994, 69: 479-493. PMID: 7994120, DOI: 10.1007/978-3-0348-7527-1_27.Peer-Reviewed Original ResearchMeSH KeywordsBiological EvolutionChromosomes, BacterialDNA, BacterialEscherichia coliPhylogenySalmonella typhimuriumSpecies SpecificityConceptsSpecies-specific sequencesSalmonella chromosomeEscherichia coliCodon usage patternsOpen reading frameHost epithelial cellsCommon ancestorMap positionPhenotypic charactersReading frameBase compositionHorizontal transferSalmonella typhimuriumMutant strainGenetic differencesEnteric speciesBacterial speciesGenomePoint mutationsPhenotypic characteristicsSpeciesCorresponding regionChromosomesSpecies differencesEpithelial cells
1993
Molecular, functional, and evolutionary analysis of sequences specific to Salmonella.
Groisman EA, Sturmoski MA, Solomon FR, Lin R, Ochman H. Molecular, functional, and evolutionary analysis of sequences specific to Salmonella. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 1033-1037. PMID: 8430070, PMCID: PMC45805, DOI: 10.1073/pnas.90.3.1033.Peer-Reviewed Original ResearchConceptsAtypical base compositionIndependent evolutionary eventsPhenotypic characteristicsOpen reading frameLac gene fusionsWild-type parentLysR familyEvolutionary eventsDeletion strainEvolutionary analysisTranscriptional regulatorsReading frameSalmonella genomeNucleotide sequenceBase compositionHorizontal transferDNA fragmentsGene fusionsBacterial speciesEnteric bacteriaStructural similaritySpeciesVirulenceSequenceUnprecedented array
1984
In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.
Groisman EA, Castilho BA, Casadaban MJ. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1480-1483. PMID: 6324195, PMCID: PMC344860, DOI: 10.1073/pnas.81.5.1480.Peer-Reviewed Original Research