2016
RNA secondary structures regulate three steps of Rho-dependent transcription termination within a bacterial mRNA leader
Kriner MA, Groisman EA. RNA secondary structures regulate three steps of Rho-dependent transcription termination within a bacterial mRNA leader. Nucleic Acids Research 2016, 45: 631-642. PMID: 28123036, PMCID: PMC5314796, DOI: 10.1093/nar/gkw889.Peer-Reviewed Original ResearchLearning from the Leaders: Gene Regulation by the Transcription Termination Factor Rho
Kriner MA, Sevostyanova A, Groisman EA. Learning from the Leaders: Gene Regulation by the Transcription Termination Factor Rho. Trends In Biochemical Sciences 2016, 41: 690-699. PMID: 27325240, PMCID: PMC4967001, DOI: 10.1016/j.tibs.2016.05.012.Peer-Reviewed Original ResearchMeSH KeywordsBacteriaGene Expression RegulationRho FactorRNA, BacterialTranscription Termination, GeneticConceptsRho-dependent terminatorsLeader regionTranscription termination factor RhoTranscription elongation complexTermination factor RhoTranscription termination eventsTranscription of genesRho-dependent terminationRho loading siteElongation complexGene regulationCellular translationBacterial mRNAsRegulatory inputsPhysiological functionsProtein synthesisTermination eventsNovel mechanismTarget specificityMultistep natureRhoLoading siteMultiple signalsTerminatorRecent studies
2015
An RNA motif advances transcription by preventing Rho-dependent termination
Sevostyanova A, Groisman EA. An RNA motif advances transcription by preventing Rho-dependent termination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: e6835-e6843. PMID: 26630006, PMCID: PMC4687561, DOI: 10.1073/pnas.1515383112.Peer-Reviewed Original ResearchConceptsRho-dependent transcription terminationTranscription terminationRNA elementsNucleic Acid Binding ProteinsRho-dependent terminationOpen reading frameRNA polymeraseReading frameBacterial RNALeader regionRNA motifsRNA conformationRho's accessInefficient translationBinding proteinInactive complexPharmacological inhibitionRhoTranscription
2014
Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator
Hollands K, Sevostiyanova A, Groisman EA. Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: e1999-e2007. PMID: 24778260, PMCID: PMC4024889, DOI: 10.1073/pnas.1319193111.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesBacterial ProteinsBase SequenceDNA-Directed RNA PolymerasesGene Expression Regulation, BacterialMagnesiumMembrane Transport ProteinsMolecular Sequence DataNucleic Acid ConformationRho FactorRiboswitchRNA, BacterialRNA, MessengerSalmonella entericaTerminator Regions, GeneticConceptsRho-dependent terminationRho-dependent terminatorsRho-dependent transcription terminatorsTranscription termination eventsTermination eventsTranscription terminationTranscription terminatorPause sitesSuch terminatorsSequence elementsAcid sequenceLeader regionNucleic acid sequencesMolecular determinantsSpecific signalsTerminatorRemarkable longevitySalmonella entericaRhoRegulationSingle site
2007
The intricate world of riboswitches
Coppins RL, Hall KB, Groisman EA. The intricate world of riboswitches. Current Opinion In Microbiology 2007, 10: 176-181. PMID: 17383225, PMCID: PMC1894890, DOI: 10.1016/j.mib.2007.03.006.Peer-Reviewed Original Research
2006
A Positive Feedback Loop Promotes Transcription Surge That Jump-Starts Salmonella Virulence Circuit
Shin D, Lee EJ, Huang H, Groisman EA. A Positive Feedback Loop Promotes Transcription Surge That Jump-Starts Salmonella Virulence Circuit. Science 2006, 314: 1607-1609. PMID: 17158330, DOI: 10.1126/science.1134930.Peer-Reviewed Original ResearchAn RNA Sensor for Intracellular Mg2+
Cromie MJ, Shi Y, Latifi T, Groisman EA. An RNA Sensor for Intracellular Mg2+. Cell 2006, 125: 71-84. PMID: 16615891, DOI: 10.1016/j.cell.2006.01.043.Peer-Reviewed Original ResearchMeSH Keywords5' Untranslated RegionsAdenosine TriphosphatasesBacterial ProteinsBiosensing TechniquesConserved SequenceCytoplasmGene Expression Regulation, BacterialMagnesiumMembrane Transport ProteinsModels, GeneticMutationNucleic Acid ConformationOpen Reading FramesPhylogenyPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidRibonucleasesRNA, BacterialRNA, MessengerTerminator Regions, GeneticTranscription, GeneticConceptsMost RNA moleculesDifferent cellular compartmentsStem-loop structureOnly protein componentSalmonella enterica serovar TyphimuriumDifferent stem-loop structuresTransporter MgtAMgtA geneEnterica serovar TyphimuriumMgtA transcriptionRNA polymeraseCellular compartmentsGene transcriptionRNA moleculesTranscription systemUntranslated regionProtein componentsTranscriptionRNA sensorsEnzymatic propertiesSerovar TyphimuriumMgtACytoplasmicDisparate stepsSingular exampleA Mg2+-responding RNA That Controls the Expression of a Mg2+ Transporter
GROISMAN EA, CROMIE MJ, SHI Y, LATIFI T. A Mg2+-responding RNA That Controls the Expression of a Mg2+ Transporter. Cold Spring Harbor Symposia On Quantitative Biology 2006, 71: 251-258. PMID: 17381304, DOI: 10.1101/sqb.2006.71.005.Peer-Reviewed Original ResearchConceptsDifferent cellular compartmentsAbundant divalent cationPhoP/PhoQStem-loop structureDifferent stem-loop structuresSensor PhoQTransporter MgtAMgtA geneMgtA transcriptionCellular compartmentsGene transcriptionTranscriptionPhoPPhoQMgtATransportersDisparate stepsBiological systemsDivalent cationsAdditional Mg2Singular exampleExpressionEnzymatic reactionsRiboswitchCytoplasmic Mg2
1999
The SPI-3 Pathogenicity Island ofSalmonella enterica
Blanc-Potard A, Solomon F, Kayser J, Groisman E. The SPI-3 Pathogenicity Island ofSalmonella enterica. Journal Of Bacteriology 1999, 181: 998-1004. PMID: 9922266, PMCID: PMC93469, DOI: 10.1128/jb.181.3.998-1004.1999.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAdhesins, Escherichia coliAmino Acid SequenceBacterial ProteinsBase CompositionCarrier ProteinsCation Transport ProteinsChromosomes, BacterialDNA PrimersDNA-Binding ProteinsEscherichia coliEvolution, MolecularMolecular Sequence DataMultigene FamilyOpen Reading FramesOperonPhylogenyPolymerase Chain ReactionRNA, BacterialRNA, TransferSalmonella entericaSequence AlignmentTranscription FactorsTranscription, GeneticVibrio choleraeVirulenceConceptsOpen reading framePathogenicity islandReading framePathogen-specific virulence genesSubspecies of SalmonellaFour-gene clusterMolecular genetic structureSalmonella enterica serovar TyphimuriumGenetic structureTranscriptional unitsChromosomal clustersEnterica serovar TyphimuriumTRNA locusSequence similaritySalmonella genomeRegulatory proteinsEnteropathogenic Escherichia coliSPI-3Escherichia coliSerovar TyphimuriumInsertion sequenceVibrio choleraeVirulence genesMultistep processSubspecies
1995
Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon
Soncini FC, Véscovi EG, Groisman EA. Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon. Journal Of Bacteriology 1995, 177: 4364-4371. PMID: 7543474, PMCID: PMC177185, DOI: 10.1128/jb.177.15.4364-4371.1995.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBase SequenceCloning, MolecularDNA Transposable ElementsGene Expression Regulation, BacterialHomeostasisLac OperonModels, GeneticMolecular Sequence DataMutationNucleic Acid ConformationOperonPromoter Regions, GeneticRNA, BacterialRNA, MessengerSalmonella typhimuriumTranscription FactorsTranscription, GeneticConceptsPhoPQ operonTranscriptional fusionsTranscriptional autoregulationPhoP-PhoQTwo-component regulatory systemAbsence of PhoPTranscriptional regulator PhoPExpression of phoPPhospho-PhoPPhoQ proteinPhoP proteinRegulator PhoPEnvironmental signalsDifferent transcriptsIndependent promotersPosttranscriptional mechanismsOperonPhoPPhoQGenesRegulatory systemBasal levelsProtein levelsProteinPromoter